ENSG00000131389

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000452151 ENSG00000131389 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC6A6 protein_coding protein_coding 8.250175 9.181851 9.717788 1.484365 0.390239 0.08175649 1.4836714 2.408376 0.1977010 0.64663414 0.1977010 -3.5414582 0.16890417 0.2541 0.01973333 -0.2343667 0.052473141 0.000144537 FALSE FALSE
ENST00000621751 ENSG00000131389 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC6A6 protein_coding protein_coding 8.250175 9.181851 9.717788 1.484365 0.390239 0.08175649 1.1118782 1.294653 0.3160907 0.02539387 0.0307927 -2.0003211 0.13901667 0.1469 0.03253333 -0.1143667 0.000144537 0.000144537 FALSE TRUE
ENST00000622186 ENSG00000131389 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC6A6 protein_coding protein_coding 8.250175 9.181851 9.717788 1.484365 0.390239 0.08175649 0.6616513 1.305861 2.7201829 1.30586148 1.4413955 1.0529900 0.07484167 0.1665 0.29113333 0.1246333 0.640523388 0.000144537 FALSE TRUE
ENST00000649500 ENSG00000131389 HEK293_OSMI2_6hA HEK293_TMG_6hB SLC6A6 protein_coding nonsense_mediated_decay 8.250175 9.181851 9.717788 1.484365 0.390239 0.08175649 4.1994295 3.227219 5.5000504 1.66023859 1.5198630 0.7673106 0.51909583 0.3351 0.55643333 0.2213333 0.828419832 0.000144537 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000131389 E001 0.3337900 0.0253040462 9.691990e-01   3 14402576 14402583 8 + 0.118 0.125 0.107
ENSG00000131389 E002 4.3919987 0.0135903160 1.119766e-01 3.023673e-01 3 14402584 14402605 22 + 0.614 0.846 0.950
ENSG00000131389 E003 6.5288160 0.0140173292 1.434717e-01 3.526162e-01 3 14402606 14402619 14 + 0.777 0.971 0.745
ENSG00000131389 E004 6.9449534 0.0085057221 1.792003e-01 4.028798e-01 3 14402620 14402620 1 + 0.819 0.986 0.634
ENSG00000131389 E005 8.1001772 0.0118052188 5.631039e-01 7.454963e-01 3 14402621 14402624 4 + 0.928 1.001 0.272
ENSG00000131389 E006 16.9151751 0.0023920882 6.596247e-01 8.097613e-01 3 14402625 14402647 23 + 1.235 1.273 0.133
ENSG00000131389 E007 39.7343499 0.0022526961 6.091057e-01 7.762805e-01 3 14402648 14402847 200 + 1.627 1.596 -0.105
ENSG00000131389 E008 0.1723744 0.0326491905 4.505169e-01   3 14411031 14411323 293 + 0.000 0.125 10.339
ENSG00000131389 E009 24.7111342 0.0051752336 2.597468e-01 4.986997e-01 3 14416412 14416453 42 + 1.452 1.364 -0.306
ENSG00000131389 E010 0.0000000       3 14432670 14432787 118 +      
ENSG00000131389 E011 18.7215908 0.0084022285 5.379184e-01 7.275646e-01 3 14443624 14443627 4 + 1.326 1.272 -0.191
ENSG00000131389 E012 65.6513458 0.0007234411 2.654055e-01 5.047738e-01 3 14443628 14443863 236 + 1.845 1.797 -0.165
ENSG00000131389 E013 0.2617363 0.3964493905 2.669733e-01   3 14443864 14444342 479 + 0.000 0.215 10.448
ENSG00000131389 E014 1.3032076 0.0148209368 5.428242e-01   3 14444738 14444866 129 + 0.407 0.302 -0.629
ENSG00000131389 E015 64.7440978 0.0007751948 4.851983e-01 6.907099e-01 3 14445717 14445851 135 + 1.830 1.799 -0.104
ENSG00000131389 E016 91.8764860 0.0006204253 7.438018e-01 8.628639e-01 3 14447582 14447816 235 + 1.973 1.961 -0.041
ENSG00000131389 E017 12.0446638 0.0027243669 4.321867e-09 2.199678e-07 3 14447817 14448188 372 + 0.703 1.324 2.315
ENSG00000131389 E018 51.3975401 0.0016073299 1.138791e-01 3.055822e-01 3 14457950 14458082 133 + 1.758 1.677 -0.272
ENSG00000131389 E019 54.2403210 0.0009338710 7.710829e-03 4.797671e-02 3 14466516 14466650 135 + 1.794 1.665 -0.437
ENSG00000131389 E020 51.3453837 0.0173834883 9.782208e-02 2.779680e-01 3 14467853 14467956 104 + 1.766 1.649 -0.395
ENSG00000131389 E021 43.0843651 0.0085297902 2.608485e-01 4.998429e-01 3 14468088 14468212 125 + 1.668 1.602 -0.224
ENSG00000131389 E022 32.2888289 0.0095460917 6.831904e-01 8.246746e-01 3 14472205 14472241 37 + 1.524 1.506 -0.062
ENSG00000131389 E023 43.6316871 0.0013662778 6.370208e-01 7.950573e-01 3 14472242 14472317 76 + 1.630 1.659 0.097
ENSG00000131389 E024 56.9987786 0.0007348819 1.817398e-01 4.062992e-01 3 14477205 14477342 138 + 1.788 1.726 -0.207
ENSG00000131389 E025 56.0468191 0.0007455200 2.520972e-02 1.128420e-01 3 14478466 14478568 103 + 1.799 1.692 -0.359
ENSG00000131389 E026 5.6279493 0.1481975353 6.874320e-01 8.274452e-01 3 14478569 14478901 333 + 0.728 0.906 0.699
ENSG00000131389 E027 58.9365473 0.0028239904 6.729460e-02 2.190312e-01 3 14479085 14479185 101 + 1.821 1.724 -0.326
ENSG00000131389 E028 82.1929569 0.0005941313 2.299311e-03 1.904853e-02 3 14481671 14481841 171 + 1.973 1.853 -0.403
ENSG00000131389 E029 944.4502831 0.0002342537 2.222378e-07 7.563344e-06 3 14484867 14489349 4483 + 2.949 2.997 0.159