ENSG00000131375

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253693 ENSG00000131375 HEK293_OSMI2_6hA HEK293_TMG_6hB CAPN7 protein_coding protein_coding 18.18405 4.500633 35.2213 0.5881636 0.8417287 2.965456 8.526542 2.647257 17.955702 0.4499637 1.0783380 2.7572357 0.54769583 0.5837667 0.50913333 -0.07463333 7.353623e-01 2.809149e-16 FALSE TRUE
ENST00000472400 ENSG00000131375 HEK293_OSMI2_6hA HEK293_TMG_6hB CAPN7 protein_coding retained_intron 18.18405 4.500633 35.2213 0.5881636 0.8417287 2.965456 1.413381 1.313718 2.290834 0.3435966 1.1850707 0.7975615 0.13356250 0.2870667 0.06646667 -0.22060000 5.250019e-01 2.809149e-16   FALSE
MSTRG.22534.5 ENSG00000131375 HEK293_OSMI2_6hA HEK293_TMG_6hB CAPN7 protein_coding   18.18405 4.500633 35.2213 0.5881636 0.8417287 2.965456 3.986910 0.000000 8.620075 0.0000000 0.5136579 9.7532294 0.13043750 0.0000000 0.24523333 0.24523333 2.809149e-16 2.809149e-16 TRUE TRUE
MSTRG.22534.9 ENSG00000131375 HEK293_OSMI2_6hA HEK293_TMG_6hB CAPN7 protein_coding   18.18405 4.500633 35.2213 0.5881636 0.8417287 2.965456 1.452782 0.000000 1.917892 0.0000000 1.0160910 7.5908806 0.04602917 0.0000000 0.05313333 0.05313333 5.667022e-01 2.809149e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000131375 E001 0.2922838 0.0257189220 1.424976e-01   3 15206152 15206220 69 + 0.048 0.275 2.911
ENSG00000131375 E002 1.0371518 0.0357042150 2.380749e-02   3 15206221 15206245 25 + 0.131 0.564 2.916
ENSG00000131375 E003 2.2346218 0.0537428453 7.279158e-03 4.596960e-02 3 15206246 15206253 8 + 0.289 0.798 2.485
ENSG00000131375 E004 3.4207690 0.0109628452 1.079423e-02 6.150864e-02 3 15206254 15206260 7 + 0.423 0.857 1.910
ENSG00000131375 E005 4.1597378 0.0063788002 1.061688e-02 6.074968e-02 3 15206261 15206262 2 + 0.494 0.907 1.741
ENSG00000131375 E006 16.1107676 0.0021450584 9.883954e-04 9.824540e-03 3 15206263 15206324 62 + 1.007 1.347 1.213
ENSG00000131375 E007 62.3406836 0.0007076733 1.893677e-04 2.556765e-03 3 15206325 15206597 273 + 1.585 1.805 0.745
ENSG00000131375 E008 1.8588433 0.0142877456 6.086210e-01 7.759773e-01 3 15210802 15210922 121 + 0.337 0.443 0.593
ENSG00000131375 E009 72.8478159 0.0009943939 6.893950e-01 8.286628e-01 3 15212104 15212212 109 + 1.686 1.656 -0.101
ENSG00000131375 E010 108.8259714 0.0035567578 6.806716e-01 8.231734e-01 3 15217425 15217582 158 + 1.859 1.828 -0.103
ENSG00000131375 E011 95.8792891 0.0006374367 1.713142e-01 3.922559e-01 3 15218473 15218540 68 + 1.809 1.726 -0.279
ENSG00000131375 E012 150.9950765 0.0302124864 1.107873e-01 3.002807e-01 3 15220781 15220981 201 + 2.013 1.848 -0.555
ENSG00000131375 E013 1.6101070 0.0599790795 9.083783e-01 9.563528e-01 3 15220982 15221031 50 + 0.314 0.275 -0.268
ENSG00000131375 E014 97.4440106 0.0005897566 1.351805e-02 7.243708e-02 3 15223475 15223561 87 + 1.823 1.673 -0.510
ENSG00000131375 E015 127.8496912 0.0003734052 9.940638e-04 9.866447e-03 3 15227839 15227949 111 + 1.943 1.768 -0.592
ENSG00000131375 E016 66.0291581 0.0005639235 1.737627e-03 1.528325e-02 3 15227950 15227965 16 + 1.665 1.426 -0.817
ENSG00000131375 E017 106.7612565 0.0005243586 1.206218e-03 1.147840e-02 3 15228974 15229059 86 + 1.864 1.673 -0.649
ENSG00000131375 E018 104.9061469 0.0004482026 2.632447e-02 1.162788e-01 3 15230442 15230535 94 + 1.854 1.726 -0.433
ENSG00000131375 E019 7.6546286 0.0278470226 5.147500e-01 7.114946e-01 3 15232111 15232158 48 + 0.789 0.659 -0.534
ENSG00000131375 E020 143.4487966 0.0006636999 3.895509e-02 1.520588e-01 3 15232519 15232665 147 + 1.986 1.882 -0.347
ENSG00000131375 E021 110.6362631 0.0004196677 4.224316e-01 6.454215e-01 3 15233867 15233973 107 + 1.868 1.823 -0.153
ENSG00000131375 E022 130.3310474 0.0005150682 6.105513e-01 7.773289e-01 3 15235025 15235145 121 + 1.932 1.952 0.068
ENSG00000131375 E023 77.1036510 0.0005977834 8.203944e-01 9.083265e-01 3 15240473 15240508 36 + 1.713 1.696 -0.057
ENSG00000131375 E024 124.2617122 0.0014912806 5.161280e-02 1.835554e-01 3 15240509 15240617 109 + 1.925 1.817 -0.365
ENSG00000131375 E025 124.0627964 0.0004059917 1.710174e-01 3.918931e-01 3 15240754 15240853 100 + 1.918 1.846 -0.244
ENSG00000131375 E026 107.3865499 0.0004492344 2.227803e-01 4.567631e-01 3 15241453 15241588 136 + 1.856 1.787 -0.232
ENSG00000131375 E027 88.0296764 0.0030936081 6.180357e-01 7.824645e-01 3 15242178 15242253 76 + 1.768 1.733 -0.116
ENSG00000131375 E028 140.5746177 0.0010571809 6.489872e-01 8.028392e-01 3 15245526 15245671 146 + 1.963 1.981 0.060
ENSG00000131375 E029 4.3847647 0.0130999723 3.976812e-03 2.902626e-02 3 15245887 15246731 845 + 0.494 0.953 1.912
ENSG00000131375 E030 103.3924851 0.0034884048 3.704183e-01 6.043123e-01 3 15246732 15246794 63 + 1.827 1.876 0.166
ENSG00000131375 E031 0.8150164 0.0292364322 1.077220e-02   3 15247237 15247326 90 + 0.092 0.563 3.497
ENSG00000131375 E032 140.1212879 0.0004399491 4.507372e-01 6.661928e-01 3 15247327 15247457 131 + 1.959 1.989 0.102
ENSG00000131375 E033 80.5711220 0.0009293785 2.006833e-01 4.305863e-01 3 15250931 15250974 44 + 1.716 1.787 0.239
ENSG00000131375 E034 89.2671307 0.0034275634 2.339846e-01 4.697317e-01 3 15250975 15251023 49 + 1.762 1.829 0.226
ENSG00000131375 E035 255.6613805 0.0002043016 3.786750e-02 1.492740e-01 3 15251116 15251414 299 + 2.215 2.278 0.208
ENSG00000131375 E036 390.1497718 0.0001857129 1.945326e-09 1.075044e-07 3 15251415 15252916 1502 + 2.386 2.527 0.470
ENSG00000131375 E037 3.4245409 0.0585392322 3.689937e-01 6.032100e-01 3 15253866 15253892 27 + 0.480 0.656 0.806