ENSG00000131323

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392745 ENSG00000131323 HEK293_OSMI2_6hA HEK293_TMG_6hB TRAF3 protein_coding protein_coding 8.716174 9.387124 7.985933 1.343871 0.09864704 -0.2329529 6.309834 6.113678 6.481633 0.5887615 0.1984347 0.08418308 0.7246167 0.6624000 0.8118667 0.1494667 2.231353e-01 9.073532e-12 FALSE TRUE
ENST00000560371 ENSG00000131323 HEK293_OSMI2_6hA HEK293_TMG_6hB TRAF3 protein_coding protein_coding 8.716174 9.387124 7.985933 1.343871 0.09864704 -0.2329529 1.023777 1.699976 0.000000 0.4605534 0.0000000 -7.41783242 0.1099792 0.1739667 0.0000000 -0.1739667 9.073532e-12 9.073532e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000131323 E001 0.6542209 0.0193874923 7.587629e-01   14 102777449 102777466 18 + 0.242 0.193 -0.418
ENSG00000131323 E002 0.9630729 0.0178602939 7.044265e-01   14 102777467 102777475 9 + 0.326 0.264 -0.421
ENSG00000131323 E003 0.9630729 0.0178602939 7.044265e-01   14 102777476 102777478 3 + 0.326 0.264 -0.421
ENSG00000131323 E004 8.6025937 0.0044542790 2.500102e-02 1.122209e-01 14 102777479 102777601 123 + 0.794 1.065 1.026
ENSG00000131323 E005 7.3899312 0.0051085019 7.132077e-02 2.274109e-01 14 102777602 102777617 16 + 0.767 0.997 0.884
ENSG00000131323 E006 17.8142654 0.0023317798 9.872802e-04 9.817478e-03 14 102777618 102777675 58 + 1.072 1.368 1.047
ENSG00000131323 E007 0.1723744 0.0421953662 6.333933e-01   14 102809008 102809129 122 + 0.000 0.107 9.587
ENSG00000131323 E008 0.1779838 0.0354442554 3.678046e-01   14 102810664 102810746 83 + 0.138 0.000 -10.933
ENSG00000131323 E009 14.0621903 0.0023268481 9.458927e-02 2.722431e-01 14 102830334 102830353 20 + 1.072 1.235 0.582
ENSG00000131323 E010 38.8536265 0.0022897110 6.698250e-01 8.161732e-01 14 102830354 102830472 119 + 1.604 1.585 -0.064
ENSG00000131323 E011 109.1938704 0.0004407940 1.671585e-02 8.441826e-02 14 102870185 102870446 262 + 2.076 2.004 -0.243
ENSG00000131323 E012 46.9576759 0.0010731863 1.105921e-01 2.999645e-01 14 102871917 102871926 10 + 1.717 1.643 -0.251
ENSG00000131323 E013 55.4310166 0.0008244769 3.944715e-02 1.533311e-01 14 102871927 102871968 42 + 1.796 1.707 -0.302
ENSG00000131323 E014 45.4490554 0.0017520283 2.135536e-01 4.458412e-01 14 102875624 102875674 51 + 1.695 1.637 -0.194
ENSG00000131323 E015 34.5525979 0.0079743939 4.888454e-02 1.769674e-01 14 102875675 102875686 12 + 1.617 1.490 -0.431
ENSG00000131323 E016 48.4561083 0.0112104285 2.511367e-01 4.892039e-01 14 102875687 102875728 42 + 1.727 1.665 -0.210
ENSG00000131323 E017 89.3622863 0.0005026151 7.253764e-02 2.298625e-01 14 102876358 102876525 168 + 1.986 1.928 -0.196
ENSG00000131323 E018 1.0523622 0.0767735052 1.360065e-01   14 102876526 102876677 152 + 0.137 0.425 2.159
ENSG00000131323 E019 58.5072807 0.0101451014 1.022740e-01 2.858664e-01 14 102886189 102886269 81 + 1.828 1.723 -0.355
ENSG00000131323 E020 50.7073756 0.0045629970 1.542198e-02 7.968499e-02 14 102889560 102889634 75 + 1.780 1.646 -0.455
ENSG00000131323 E021 68.1154639 0.0006207966 1.398155e-04 1.978493e-03 14 102891325 102891417 93 + 1.917 1.763 -0.521
ENSG00000131323 E022 2.5743812 0.0180584003 5.495244e-01 7.359512e-01 14 102895073 102895199 127 + 0.600 0.509 -0.419
ENSG00000131323 E023 100.4010417 0.0004181106 1.952538e-04 2.623423e-03 14 102897261 102897401 141 + 2.067 1.945 -0.409
ENSG00000131323 E024 136.3613146 0.0003755911 3.940213e-02 1.532297e-01 14 102903255 102903429 175 + 2.162 2.108 -0.181
ENSG00000131323 E025 1279.2063310 0.0003641338 2.893310e-14 3.968906e-12 14 102905213 102911500 6288 + 3.063 3.130 0.222