ENSG00000130749

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253048 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding protein_coding 15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 0.8973024 2.81884939 0.0000000 0.50867466 0.00000000 -8.144072 0.05187917 0.13533333 0.00000000 -0.13533333 1.233432e-15 4.00131e-20 FALSE TRUE
ENST00000597069 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding processed_transcript 15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 0.9372184 1.16353305 0.5083995 0.07137719 0.12772292 -1.178722 0.06479167 0.05750000 0.04543333 -0.01206667 8.163530e-01 4.00131e-20 FALSE FALSE
ENST00000601973 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding protein_coding 15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 2.1637674 3.53793353 0.0000000 0.90920432 0.00000000 -8.470835 0.11775417 0.17010000 0.00000000 -0.17010000 3.604533e-12 4.00131e-20 FALSE TRUE
MSTRG.17391.1 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 1.2754404 0.91611367 0.0000000 0.91611367 0.00000000 -6.533117 0.07030417 0.04146667 0.00000000 -0.04146667 9.658040e-01 4.00131e-20 FALSE TRUE
MSTRG.17391.10 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 0.4878324 0.05349539 1.3701119 0.05349539 0.06711555 4.441990 0.03898750 0.00330000 0.12676667 0.12346667 9.742862e-04 4.00131e-20 FALSE TRUE
MSTRG.17391.3 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 1.9268002 0.79569863 3.1321548 0.79569863 1.04927727 1.963442 0.15126667 0.03456667 0.27566667 0.24110000 1.518578e-01 4.00131e-20 FALSE TRUE
MSTRG.17391.5 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 5.7847193 9.17279571 4.0571485 0.98264022 0.34674186 -1.174916 0.38312083 0.45683333 0.38150000 -0.07533333 8.288543e-01 4.00131e-20 FALSE TRUE
MSTRG.17391.6 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 0.7605865 1.69866627 0.0000000 0.15873902 0.00000000 -7.416727 0.04520000 0.08323333 0.00000000 -0.08323333 8.218896e-14 4.00131e-20 FALSE TRUE
MSTRG.17391.8 ENSG00000130749 HEK293_OSMI2_6hA HEK293_TMG_6hB ZC3H4 protein_coding   15.17832 20.45797 10.8767 2.105753 0.8906641 -0.9108016 0.8441514 0.00000000 1.6661699 0.00000000 0.08161636 7.389025 0.06920833 0.00000000 0.15596667 0.15596667 4.001310e-20 4.00131e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130749 E001 0.2027342 0.0422367248 1.0000000000   19 47063906 47064186 281 - 0.000 0.089 13.948
ENSG00000130749 E002 2.3575094 0.0097551781 0.0069038137 0.0441994767 19 47064187 47064191 5 - 0.000 0.620 17.751
ENSG00000130749 E003 16.5179573 0.1090610027 0.3506859232 0.5878503937 19 47064192 47064232 41 - 1.018 1.292 0.980
ENSG00000130749 E004 307.0888471 1.1027903524 0.2923925529 0.5331048013 19 47064233 47064858 626 - 2.133 2.559 1.420
ENSG00000130749 E005 879.2443449 1.5786886338 0.3970628238 0.6259923337 19 47064859 47067321 2463 - 2.646 3.004 1.189
ENSG00000130749 E006 222.9959927 1.0694072529 0.4073372901 0.6339605614 19 47067322 47067869 548 - 2.102 2.398 0.990
ENSG00000130749 E007 105.9386444 0.0004722387 0.1068797153 0.2938231924 19 47069092 47069343 252 - 1.864 2.061 0.662
ENSG00000130749 E008 131.9806609 0.0100501242 0.1645136480 0.3833925875 19 47071778 47072121 344 - 2.051 2.117 0.222
ENSG00000130749 E009 98.6371976 0.0072383470 0.0049297517 0.0342547147 19 47072352 47072713 362 - 1.982 1.968 -0.048
ENSG00000130749 E010 0.9529514 0.2812639056 0.2335270973   19 47073500 47073568 69 - 0.000 0.341 16.343
ENSG00000130749 E011 88.9060416 0.0010605873 0.0000466933 0.0007844095 19 47081513 47081622 110 - 1.955 1.921 -0.114
ENSG00000130749 E012 116.4287209 0.0111280060 0.0005704935 0.0063140585 19 47082184 47082295 112 - 2.084 2.022 -0.208
ENSG00000130749 E013 107.4489118 0.0214182171 0.0124784774 0.0683480880 19 47084345 47084455 111 - 2.038 1.992 -0.153
ENSG00000130749 E014 108.7629649 0.0224407773 0.0066505768 0.0429479454 19 47085056 47085132 77 - 2.055 1.991 -0.216
ENSG00000130749 E015 96.0524006 0.0355603170 0.0128516679 0.0698268748 19 47085133 47085195 63 - 2.017 1.928 -0.298
ENSG00000130749 E016 106.3155231 0.0275070504 0.0102203383 0.0590620637 19 47085318 47085414 97 - 2.049 1.979 -0.234
ENSG00000130749 E017 90.8222217 0.0224786600 0.0688144266 0.2223965662 19 47086384 47086538 155 - 1.933 1.936 0.008
ENSG00000130749 E018 58.9886641 0.0183917477 0.0818544106 0.2484896869 19 47089967 47090018 52 - 1.746 1.753 0.023
ENSG00000130749 E019 122.8413982 0.0220184432 0.0802554323 0.2453283824 19 47090019 47090189 171 - 2.055 2.071 0.055
ENSG00000130749 E020 1.0994779 0.0389983374 0.8980479599   19 47093749 47093969 221 - 0.283 0.328 0.296
ENSG00000130749 E021 112.1993082 0.0087092406 0.0018511853 0.0160634388 19 47093970 47094080 111 - 2.047 2.017 -0.099
ENSG00000130749 E022 157.4487714 0.0126031418 0.0007539728 0.0078911575 19 47094389 47094608 220 - 2.208 2.155 -0.179
ENSG00000130749 E023 3.4164765 0.0071385550 0.7781359079 0.8835963885 19 47110895 47110956 62 - 0.518 0.665 0.656
ENSG00000130749 E024 38.0681450 0.0430364519 0.0256431967 0.1142004194 19 47112148 47112294 147 - 1.633 1.529 -0.358
ENSG00000130749 E025 19.1314519 0.0017969070 0.0086002140 0.0520478293 19 47112424 47112589 166 - 1.336 1.249 -0.305
ENSG00000130749 E026 24.0933242 0.0106859230 0.0003858746 0.0045953986 19 47112714 47113092 379 - 1.477 1.311 -0.577
ENSG00000130749 E027 5.6597131 0.0449763011 0.4061704455 0.6331781488 19 47113720 47113795 76 - 0.813 0.793 -0.081