ENSG00000130731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000301686 ENSG00000130731 HEK293_OSMI2_6hA HEK293_TMG_6hB METTL26 protein_coding protein_coding 258.1548 393.9534 75.65691 92.41308 3.514962 -2.380327 29.07415 33.49516 9.676755 7.788857 0.4077659 -1.790298 0.13270000 0.0850000 0.1286000 0.043600000 5.677601e-02 1.938779e-11 FALSE TRUE
ENST00000338401 ENSG00000130731 HEK293_OSMI2_6hA HEK293_TMG_6hB METTL26 protein_coding protein_coding 258.1548 393.9534 75.65691 92.41308 3.514962 -2.380327 95.59010 159.76521 18.287026 36.065323 1.2911229 -3.126363 0.32567083 0.4077333 0.2412000 -0.166533333 1.938779e-11 1.938779e-11 FALSE TRUE
ENST00000397664 ENSG00000130731 HEK293_OSMI2_6hA HEK293_TMG_6hB METTL26 protein_coding protein_coding 258.1548 393.9534 75.65691 92.41308 3.514962 -2.380327 42.25733 68.34674 12.996653 21.853083 0.1874550 -2.393834 0.15642083 0.1638000 0.1723667 0.008566667 9.585396e-01 1.938779e-11 FALSE TRUE
ENST00000448973 ENSG00000130731 HEK293_OSMI2_6hA HEK293_TMG_6hB METTL26 protein_coding retained_intron 258.1548 393.9534 75.65691 92.41308 3.514962 -2.380327 17.24544 23.00641 11.333336 2.358071 1.2538342 -1.020818 0.09510417 0.0698000 0.1489333 0.079133333 2.453200e-01 1.938779e-11 TRUE FALSE
ENST00000568077 ENSG00000130731 HEK293_OSMI2_6hA HEK293_TMG_6hB METTL26 protein_coding nonsense_mediated_decay 258.1548 393.9534 75.65691 92.41308 3.514962 -2.380327 36.75288 52.97027 14.822564 14.115185 1.2329048 -1.836687 0.14546250 0.1315667 0.1952667 0.063700000 2.418534e-02 1.938779e-11 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130731 E001 1.911155 2.2909848743 6.751350e-01 8.196231e-01 16 634427 634428 2 - 0.259 0.366 0.696
ENSG00000130731 E002 15.669832 0.6083561994 1.000000e+00 1.000000e+00 16 634429 634429 1 - 0.876 1.073 0.732
ENSG00000130731 E003 104.118601 0.0068072002 4.343424e-01 6.545039e-01 16 634430 634435 6 - 1.793 1.855 0.210
ENSG00000130731 E004 112.993254 0.0064358287 3.102568e-01 5.507403e-01 16 634436 634437 2 - 1.816 1.892 0.255
ENSG00000130731 E005 451.243431 0.0057574861 5.310170e-01 7.227918e-01 16 634438 634501 64 - 2.444 2.479 0.118
ENSG00000130731 E006 621.519230 0.0011054544 1.263287e-01 3.259869e-01 16 634502 634512 11 - 2.584 2.621 0.124
ENSG00000130731 E007 610.938006 0.0004739391 4.813596e-02 1.753181e-01 16 634513 634525 13 - 2.575 2.616 0.136
ENSG00000130731 E008 1225.192657 0.0005663800 1.055136e-01 2.915165e-01 16 634526 634625 100 - 2.890 2.914 0.078
ENSG00000130731 E009 992.908183 0.0001108002 3.806954e-01 6.127692e-01 16 634626 634644 19 - 2.817 2.826 0.029
ENSG00000130731 E010 24.278083 0.0330338627 2.805316e-01 5.207376e-01 16 634664 634718 55 - 1.361 1.207 -0.540
ENSG00000130731 E011 891.105186 0.0004317785 8.257790e-01 9.113648e-01 16 634719 634736 18 - 2.784 2.774 -0.033
ENSG00000130731 E012 1299.021548 0.0005814515 1.829324e-01 4.077762e-01 16 634737 634797 61 - 2.920 2.939 0.062
ENSG00000130731 E013 553.747612 0.0009509864 1.395002e-07 5.012768e-06 16 634889 634948 60 - 2.690 2.546 -0.479
ENSG00000130731 E014 361.738366 0.0011676296 5.032867e-07 1.556824e-05 16 634949 634956 8 - 2.522 2.360 -0.541
ENSG00000130731 E015 292.740502 0.0232811872 1.367768e-02 7.307800e-02 16 634957 635063 107 - 2.472 2.253 -0.729
ENSG00000130731 E016 271.167950 0.0386095681 2.649992e-02 1.167725e-01 16 635064 635280 217 - 2.459 2.215 -0.814
ENSG00000130731 E017 661.569009 0.0030643157 8.141539e-02 2.476439e-01 16 635281 635340 60 - 2.693 2.634 -0.197
ENSG00000130731 E018 82.800660 0.0938113796 8.554750e-02 2.556090e-01 16 635341 635517 177 - 1.984 1.696 -0.969
ENSG00000130731 E019 84.276483 0.0603572767 3.840051e-01 6.155760e-01 16 635518 635611 94 - 1.861 1.734 -0.429
ENSG00000130731 E020 455.123218 0.0047702146 1.000000e+00 1.000000e+00 16 635612 635774 163 - 2.488 2.479 -0.031
ENSG00000130731 E021 103.793334 0.1758158952 1.522801e-01 3.656974e-01 16 635775 636093 319 - 2.078 1.793 -0.958
ENSG00000130731 E022 1368.125778 0.0030594173 1.771805e-12 1.742288e-10 16 636094 636366 273 - 2.749 2.978 0.760