ENSG00000130725

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253023 ENSG00000130725 HEK293_OSMI2_6hA HEK293_TMG_6hB UBE2M protein_coding protein_coding 320.8433 454.7459 167.1014 92.00498 9.835958 -1.444283 195.47836 255.77604 82.25292 66.755647 5.813970 -1.6366233 0.5768792 0.5444000 0.49153333 -0.05286667 0.8014116340 0.0003860231 FALSE  
ENST00000595957 ENSG00000130725 HEK293_OSMI2_6hA HEK293_TMG_6hB UBE2M protein_coding protein_coding 320.8433 454.7459 167.1014 92.00498 9.835958 -1.444283 37.05726 59.83865 38.29339 9.637063 2.447749 -0.6438466 0.1425083 0.1346000 0.22926667 0.09466667 0.0003860231 0.0003860231    
MSTRG.17868.1 ENSG00000130725 HEK293_OSMI2_6hA HEK293_TMG_6hB UBE2M protein_coding   320.8433 454.7459 167.1014 92.00498 9.835958 -1.444283 40.39122 74.56822 11.17916 15.480622 1.904455 -2.7366520 0.1079250 0.1634333 0.06786667 -0.09556667 0.0181639979 0.0003860231 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130725 E001 2631.307469 9.842657e-04 1.594218e-06 4.274312e-05 19 58555712 58556140 429 - 3.262 3.353 0.301
ENSG00000130725 E002 2316.570016 2.114638e-04 1.724603e-01 3.937771e-01 19 58556141 58556200 60 - 3.260 3.287 0.089
ENSG00000130725 E003 2347.539333 1.499496e-04 9.264381e-01 9.653182e-01 19 58556201 58556229 29 - 3.278 3.289 0.036
ENSG00000130725 E004 71.517602 5.018312e-02 1.097029e-01 2.985396e-01 19 58556230 58556315 86 - 1.931 1.721 -0.707
ENSG00000130725 E005 2644.600827 1.786782e-04 8.416867e-01 9.204273e-01 19 58556316 58556379 64 - 3.331 3.340 0.029
ENSG00000130725 E006 80.663104 8.014514e-02 8.813587e-02 2.602640e-01 19 58556380 58556490 111 - 2.009 1.761 -0.835
ENSG00000130725 E007 29.535718 1.255019e-01 2.126306e-01 4.448197e-01 19 58556582 58556593 12 - 1.568 1.344 -0.771
ENSG00000130725 E008 48.115761 1.812002e-01 1.400844e-01 3.476480e-01 19 58556594 58556686 93 - 1.814 1.528 -0.971
ENSG00000130725 E009 3119.129500 1.599432e-04 3.720704e-07 1.192191e-05 19 58556687 58556790 104 - 3.436 3.402 -0.113
ENSG00000130725 E010 42.177481 1.736524e-03 4.209647e-02 1.601611e-01 19 58556791 58556794 4 - 1.642 1.523 -0.407
ENSG00000130725 E011 1897.984230 1.945048e-04 5.413908e-07 1.661377e-05 19 58556892 58556930 39 - 3.229 3.184 -0.150
ENSG00000130725 E012 16.851680 5.459618e-02 2.751008e-02 1.198185e-01 19 58556931 58557062 132 - 1.396 1.079 -1.118
ENSG00000130725 E013 2185.760187 6.134392e-05 1.071899e-07 3.943525e-06 19 58557063 58557157 95 - 3.286 3.250 -0.117
ENSG00000130725 E014 7.116726 2.889028e-01 1.312899e-01 3.338380e-01 19 58557968 58558272 305 - 1.117 0.711 -1.548
ENSG00000130725 E015 1135.064906 5.749651e-04 1.343328e-02 7.212332e-02 19 58558273 58558354 82 - 2.995 2.966 -0.094
ENSG00000130725 E016 621.268650 1.634857e-03 1.495732e-02 7.792663e-02 19 58558355 58558519 165 - 2.650 2.726 0.254
ENSG00000130725 E017 321.204728 1.298870e-02 2.327616e-07 7.877296e-06 19 58558520 58558658 139 - 2.138 2.476 1.130
ENSG00000130725 E018 273.790140 4.711257e-03 7.466314e-08 2.858967e-06 19 58558659 58558693 35 - 2.147 2.397 0.834
ENSG00000130725 E019 575.081602 5.826802e-03 1.210539e-04 1.750488e-03 19 58558694 58558954 261 - 2.541 2.705 0.548