ENSG00000130402

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252699 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding protein_coding 142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 24.804612 36.73727 13.65687 3.9046635 0.7355483 -1.4269541 0.16495833 0.19423333 0.1100000 -0.084233333 3.526413e-03 6.543549e-63 FALSE TRUE
ENST00000390009 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding protein_coding 142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 9.395743 15.11020 0.00000 2.3788397 0.0000000 -10.5622618 0.05625417 0.07936667 0.0000000 -0.079366667 1.707311e-33 6.543549e-63 FALSE TRUE
ENST00000424234 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding protein_coding 142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 18.639010 24.88245 19.20857 1.4346270 1.3042100 -0.3732068 0.13512917 0.13290000 0.1550333 0.022133333 7.887672e-01 6.543549e-63 FALSE TRUE
ENST00000495553 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding processed_transcript 142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 2.968273 0.00000 15.03054 0.0000000 0.7708705 10.5546402 0.02424583 0.00000000 0.1208000 0.120800000 6.543549e-63 6.543549e-63 FALSE FALSE
MSTRG.17094.1 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding   142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 13.637295 20.04363 12.77367 0.8025475 1.3148211 -0.6495614 0.09446250 0.10696667 0.1033667 -0.003600000 9.748913e-01 6.543549e-63 FALSE TRUE
MSTRG.17094.2 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding   142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 38.211721 52.56680 34.10467 9.5476810 2.1811457 -0.6240340 0.27647083 0.27570000 0.2738333 -0.001866667 1.000000e+00 6.543549e-63 FALSE TRUE
MSTRG.17094.9 ENSG00000130402 HEK293_OSMI2_6hA HEK293_TMG_6hB ACTN4 protein_coding   142.3814 188.2254 124.2852 10.81192 4.172142 -0.5987677 20.448156 25.47587 20.30449 2.8680955 2.2150268 -0.3271883 0.15015417 0.13743333 0.1625333 0.025100000 8.255975e-01 6.543549e-63 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130402 E001 6.8398525 4.770730e-03 2.437173e-01 4.807408e-01 19 38647641 38647643 3 + 0.748 0.917 0.659
ENSG00000130402 E002 9.7989302 2.824329e-02 8.715659e-02 2.585542e-01 19 38647644 38647648 5 + 0.814 1.074 0.976
ENSG00000130402 E003 13.4294071 2.826268e-03 4.836071e-03 3.372381e-02 19 38647649 38647659 11 + 0.897 1.215 1.161
ENSG00000130402 E004 20.5119930 2.083571e-03 6.240841e-02 2.081747e-01 19 38647660 38647669 10 + 1.183 1.357 0.612
ENSG00000130402 E005 211.3740438 2.267657e-04 2.846789e-02 1.227062e-01 19 38647670 38647737 68 + 2.249 2.323 0.249
ENSG00000130402 E006 698.5155533 1.628563e-03 8.528132e-01 9.265696e-01 19 38647738 38647907 170 + 2.804 2.824 0.069
ENSG00000130402 E007 0.3032425 2.444117e-02 4.880458e-01   19 38648193 38648260 68 + 0.000 0.162 8.892
ENSG00000130402 E008 975.0033463 1.114862e-03 6.167139e-03 4.055458e-02 19 38700600 38700714 115 + 2.987 2.949 -0.127
ENSG00000130402 E009 1197.9217590 8.975334e-05 1.103621e-17 2.399747e-15 19 38701002 38701121 120 + 3.104 3.023 -0.268
ENSG00000130402 E010 1163.9189619 4.493271e-04 3.822086e-10 2.396891e-08 19 38704934 38705020 87 + 3.088 3.011 -0.254
ENSG00000130402 E011 1141.0549144 3.359511e-04 2.135464e-15 3.424465e-13 19 38706044 38706131 88 + 3.090 2.996 -0.313
ENSG00000130402 E012 1069.7504083 1.035628e-04 9.989722e-21 3.206965e-18 19 38708117 38708195 79 + 3.064 2.968 -0.320
ENSG00000130402 E013 1063.8007263 1.293884e-04 8.332633e-13 8.649589e-11 19 38709395 38709476 82 + 3.047 2.974 -0.241
ENSG00000130402 E014 918.8739336 9.500835e-04 6.951714e-04 7.403493e-03 19 38710257 38710342 86 + 2.968 2.918 -0.165
ENSG00000130402 E015 52.8268413 1.040683e-01 2.656503e-02 1.169714e-01 19 38710833 38711275 443 + 1.884 1.580 -1.030
ENSG00000130402 E016 82.5338251 1.420616e-02 7.901190e-03 4.886700e-02 19 38711276 38711361 86 + 2.000 1.832 -0.564
ENSG00000130402 E017 941.1608583 3.295408e-04 7.958433e-03 4.912671e-02 19 38714469 38714561 93 + 2.962 2.938 -0.080
ENSG00000130402 E018 1346.5415504 3.898897e-04 6.739244e-01 8.188475e-01 19 38717086 38717316 231 + 3.087 3.108 0.068
ENSG00000130402 E019 1333.4178268 4.043077e-04 3.763616e-01 6.092990e-01 19 38717927 38718074 148 + 3.095 3.098 0.009
ENSG00000130402 E020 1401.9163494 7.351167e-05 2.932130e-02 1.253478e-01 19 38721538 38721688 151 + 3.124 3.117 -0.024
ENSG00000130402 E021 4.7186837 2.153763e-01 9.202210e-01 9.623300e-01 19 38721689 38721878 190 + 0.665 0.759 0.384
ENSG00000130402 E022 1077.2533507 3.646869e-04 1.337145e-01 3.376328e-01 19 38723614 38723722 109 + 3.009 3.003 -0.019
ENSG00000130402 E023 1080.2544616 9.578983e-05 7.162759e-01 8.457809e-01 19 38723937 38724077 141 + 2.993 3.013 0.066
ENSG00000130402 E024 1336.0122157 3.679600e-04 1.083735e-01 2.963514e-01 19 38724157 38724339 183 + 3.073 3.110 0.122
ENSG00000130402 E025 1214.7162223 6.249135e-04 4.308926e-02 1.628220e-01 19 38724431 38724565 135 + 3.024 3.072 0.158
ENSG00000130402 E026 3.9869449 6.527887e-03 9.181010e-01 9.612633e-01 19 38725589 38725723 135 + 0.670 0.666 -0.016
ENSG00000130402 E027 560.7658652 2.656891e-03 7.366044e-01 8.583374e-01 19 38725724 38725726 3 + 2.704 2.729 0.084
ENSG00000130402 E028 1467.0133113 6.790896e-04 1.434464e-01 3.525820e-01 19 38725727 38725903 177 + 3.113 3.151 0.126
ENSG00000130402 E029 1379.3496400 7.031598e-04 7.560218e-02 2.360282e-01 19 38726957 38727103 147 + 3.082 3.127 0.147
ENSG00000130402 E030 19.5272135 1.157079e-01 8.079548e-01 9.009973e-01 19 38727856 38727945 90 + 1.272 1.292 0.071
ENSG00000130402 E031 1123.7963728 3.582311e-04 1.199820e-02 6.643313e-02 19 38727946 38728026 81 + 2.988 3.039 0.172
ENSG00000130402 E032 6.3396970 1.790231e-01 2.739129e-01 5.138435e-01 19 38728145 38728315 171 + 0.949 0.778 -0.659
ENSG00000130402 E033 19.1421870 2.330006e-03 5.615282e-01 7.443416e-01 19 38728316 38728381 66 + 1.232 1.297 0.227
ENSG00000130402 E034 1526.4847134 1.013600e-03 4.809546e-04 5.503481e-03 19 38728996 38729154 159 + 3.101 3.180 0.261
ENSG00000130402 E035 2868.0622973 3.423675e-04 4.486955e-23 1.872207e-20 19 38729274 38729988 715 + 3.343 3.467 0.410
ENSG00000130402 E036 893.2970668 3.888218e-02 1.274623e-03 1.197038e-02 19 38729989 38731589 1601 + 2.692 3.002 1.032