ENSG00000130363

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367069 ENSG00000130363 HEK293_OSMI2_6hA HEK293_TMG_6hB RSPH3 protein_coding protein_coding 3.459302 3.210323 3.988542 0.6037904 0.1564985 0.3122684 1.2725479 0.4702612 2.10742375 0.03639725 0.2078326 2.1404188 0.3441917 0.1559000 0.5278333 0.3719333 0.0001602958 0.0001602958 FALSE TRUE
ENST00000449822 ENSG00000130363 HEK293_OSMI2_6hA HEK293_TMG_6hB RSPH3 protein_coding protein_coding 3.459302 3.210323 3.988542 0.6037904 0.1564985 0.3122684 0.3051574 0.4716148 0.09489626 0.27842879 0.0549410 -2.1989166 0.0891000 0.1298333 0.0235000 -0.1063333 0.2949787090 0.0001602958 FALSE TRUE
MSTRG.29266.1 ENSG00000130363 HEK293_OSMI2_6hA HEK293_TMG_6hB RSPH3 protein_coding   3.459302 3.210323 3.988542 0.6037904 0.1564985 0.3122684 1.7509363 2.2598870 1.58765718 0.35525705 0.1808054 -0.5066626 0.5342417 0.7122667 0.4005000 -0.3117667 0.0164284383 0.0001602958 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130363 E001 0.3807181 0.14226899 0.878441652   6 158972494 158972593 100 - 0.112 0.133 0.280
ENSG00000130363 E002 62.8315110 0.76495830 0.935784969 0.9700643 6 158972871 158975390 2520 - 1.909 1.634 -0.927
ENSG00000130363 E003 18.0559444 0.40147341 0.453073517 0.6680005 6 158975391 158975922 532 - 1.438 0.964 -1.685
ENSG00000130363 E004 17.1046157 0.36731876 0.616110046 0.7811098 6 158975923 158976581 659 - 1.390 1.023 -1.301
ENSG00000130363 E005 7.3480572 0.55108333 0.130178174 0.3320954 6 158976582 158976833 252 - 1.117 0.445 -2.760
ENSG00000130363 E006 5.5190032 0.27646543 0.220218768 0.4537895 6 158976834 158977148 315 - 0.967 0.536 -1.763
ENSG00000130363 E007 12.8912276 0.08344057 0.036294206 0.1450291 6 158977149 158977482 334 - 1.012 1.269 0.920
ENSG00000130363 E008 45.9295933 0.48612475 0.296590069 0.5370974 6 158977483 158977848 366 - 1.626 1.719 0.315
ENSG00000130363 E009 20.0954659 0.00181487 0.265587956 0.5049834 6 158978260 158978346 87 - 1.309 1.361 0.182
ENSG00000130363 E010 29.8631012 0.04369806 0.173849389 0.3956477 6 158980774 158980936 163 - 1.554 1.393 -0.555
ENSG00000130363 E011 39.6075817 0.09426209 0.299219348 0.5396526 6 158982485 158982688 204 - 1.663 1.531 -0.451
ENSG00000130363 E012 27.2554519 0.06611203 0.754587096 0.8694542 6 158983662 158983807 146 - 1.466 1.430 -0.124
ENSG00000130363 E013 23.4120994 0.07679031 0.911884152 0.9581029 6 158986280 158986421 142 - 1.389 1.384 -0.016
ENSG00000130363 E014 18.8656293 0.06108076 0.989993464 0.9967253 6 158993839 158993926 88 - 1.294 1.306 0.040
ENSG00000130363 E015 77.1828234 0.05780588 0.001061995 0.0103865 6 158999435 159000202 768 - 1.695 2.049 1.193