ENSG00000130066

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000379253 ENSG00000130066 HEK293_OSMI2_6hA HEK293_TMG_6hB SAT1 protein_coding retained_intron 17.32725 9.954137 13.85057 0.9113175 1.181621 0.4761694 2.069148 1.6075537 2.0071309 0.2225437 0.13289100 0.3184912 0.12784583 0.16720000 0.1489 -0.018300000 0.95840291 0.01460265 FALSE  
ENST00000379270 ENSG00000130066 HEK293_OSMI2_6hA HEK293_TMG_6hB SAT1 protein_coding protein_coding 17.32725 9.954137 13.85057 0.9113175 1.181621 0.4761694 10.726803 5.8488660 8.5274820 1.1000997 0.59120300 0.5431892 0.60215000 0.57643333 0.6180 0.041566667 0.91665460 0.01460265 FALSE  
ENST00000474223 ENSG00000130066 HEK293_OSMI2_6hA HEK293_TMG_6hB SAT1 protein_coding retained_intron 17.32725 9.954137 13.85057 0.9113175 1.181621 0.4761694 2.592523 1.7010182 2.5393923 0.2776736 0.62828821 0.5752983 0.16380000 0.17913333 0.1779 -0.001233333 1.00000000 0.01460265 FALSE  
ENST00000489394 ENSG00000130066 HEK293_OSMI2_6hA HEK293_TMG_6hB SAT1 protein_coding nonsense_mediated_decay 17.32725 9.954137 13.85057 0.9113175 1.181621 0.4761694 1.354754 0.7278447 0.1310236 0.2507379 0.03647847 -2.3873811 0.06866667 0.07076667 0.0101 -0.060666667 0.01460265 0.01460265    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000130066 E001 11.4513167 0.0316269308 0.049032860 0.17727648 X 23783173 23783196 24 + 1.222 0.976 -0.890
ENSG00000130066 E002 37.7391277 0.0045637835 0.054030476 0.18914509 X 23783197 23783277 81 + 1.659 1.531 -0.438
ENSG00000130066 E003 55.3653563 0.0011358184 0.373517563 0.60696291 X 23783278 23783417 140 + 1.783 1.732 -0.174
ENSG00000130066 E004 0.6190593 1.6540150596 0.247955390   X 23783508 23783657 150 + 0.305 0.115 -1.754
ENSG00000130066 E005 35.6603435 0.0015218157 0.632091831 0.79174305 X 23783658 23783709 52 + 1.556 1.574 0.060
ENSG00000130066 E006 1.3470695 0.5549885620 0.046598405   X 23783710 23783799 90 + 0.575 0.109 -3.278
ENSG00000130066 E007 41.6311871 0.0009799412 0.084922174 0.25446510 X 23783800 23783883 84 + 1.589 1.670 0.276
ENSG00000130066 E008 25.7132162 0.0642143173 1.000000000 1.00000000 X 23783884 23784293 410 + 1.416 1.441 0.087
ENSG00000130066 E009 15.6774668 0.0022762446 0.642653006 0.79865725 X 23784294 23784403 110 + 1.210 1.242 0.113
ENSG00000130066 E010 7.2986697 0.0050100787 0.033797383 0.13832296 X 23784404 23784411 8 + 1.051 0.798 -0.956
ENSG00000130066 E011 38.2135979 0.0589033954 0.389741548 0.62026933 X 23784753 23785259 507 + 1.646 1.549 -0.328
ENSG00000130066 E012 13.9402070 0.0042947526 0.381429822 0.61342232 X 23785260 23785327 68 + 1.137 1.212 0.264
ENSG00000130066 E013 61.2416228 0.0007494588 0.002530774 0.02053139 X 23785328 23785429 102 + 1.730 1.851 0.408
ENSG00000130066 E014 1.7300004 0.1877236665 0.049205127 0.17769310 X 23785430 23785519 90 + 0.619 0.201 -2.426
ENSG00000130066 E015 38.9568372 0.0064311129 0.940510630 0.97254056 X 23785520 23785560 41 + 1.614 1.602 -0.041
ENSG00000130066 E016 102.8148731 0.0024048761 0.478926341 0.68639831 X 23785686 23786210 525 + 2.015 2.034 0.063