ENSG00000127993

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265732 ENSG00000127993 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM48 protein_coding protein_coding 6.60847 2.561684 12.56454 0.3709661 0.4262096 2.289721 1.5744408 1.2581930 1.9799310 0.29465985 0.1221476 0.6499441 0.31198333 0.47593333 0.1573000 -0.318633333 5.691852e-05 5.691852e-05 FALSE TRUE
ENST00000481551 ENSG00000127993 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM48 protein_coding protein_coding 6.60847 2.561684 12.56454 0.3709661 0.4262096 2.289721 1.3594191 0.3150296 3.2630318 0.05685572 0.2328791 3.3319847 0.18596667 0.13573333 0.2613333 0.125600000 3.600204e-01 5.691852e-05 FALSE TRUE
ENST00000496410 ENSG00000127993 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM48 protein_coding protein_coding 6.60847 2.561684 12.56454 0.3709661 0.4262096 2.289721 0.4727261 0.1166190 0.7354013 0.11661903 0.2514590 2.5575231 0.06214167 0.06386667 0.0587000 -0.005166667 7.709245e-01 5.691852e-05   FALSE
MSTRG.30209.3 ENSG00000127993 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM48 protein_coding   6.60847 2.561684 12.56454 0.3709661 0.4262096 2.289721 3.1102079 0.8265825 6.4926191 0.21769836 0.5400230 2.9584411 0.41926250 0.30913333 0.5150333 0.205900000 1.312842e-01 5.691852e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000127993 E001 0.9228518 0.2445450374 4.663048e-01   7 92528760 92528772 13 + 0.193 0.337 1.065
ENSG00000127993 E002 1.5357542 0.0134423068 4.837571e-01 6.896169e-01 7 92528773 92528783 11 + 0.323 0.432 0.626
ENSG00000127993 E003 1.8398045 0.0113615720 7.402272e-01 8.605630e-01 7 92528784 92528793 10 + 0.393 0.432 0.212
ENSG00000127993 E004 1.9706726 0.0106099537 4.206356e-01 6.441669e-01 7 92528794 92528794 1 + 0.393 0.514 0.626
ENSG00000127993 E005 3.9305742 0.0090307672 8.189146e-01 9.073965e-01 7 92528795 92528806 12 + 0.634 0.644 0.040
ENSG00000127993 E006 40.1947129 0.0014991930 2.700646e-01 5.100565e-01 7 92528807 92528924 118 + 1.577 1.467 -0.375
ENSG00000127993 E007 3.5144007 0.0914142692 3.826127e-01 6.143589e-01 7 92528925 92529183 259 + 0.651 0.433 -1.020
ENSG00000127993 E008 65.5390334 0.0007133458 3.878959e-01 6.188423e-01 7 92529476 92529666 191 + 1.760 1.765 0.018
ENSG00000127993 E009 29.6965910 0.0012443171 1.130992e-01 3.043343e-01 7 92532404 92532408 5 + 1.408 1.484 0.260
ENSG00000127993 E010 65.8900716 0.0007486257 5.009565e-03 3.467169e-02 7 92532409 92532549 141 + 1.736 1.832 0.324
ENSG00000127993 E011 0.4868358 0.2109531338 6.871710e-01   7 92532550 92532550 1 + 0.137 0.193 0.586
ENSG00000127993 E012 196.8895310 0.0073983001 9.432439e-01 9.739669e-01 7 92534402 92534970 569 + 2.242 2.211 -0.105
ENSG00000127993 E013 97.4179592 0.0492687597 6.359389e-05 1.023433e-03 7 92534971 92536433 1463 + 2.012 1.512 -1.690
ENSG00000127993 E014 17.0931183 0.0021835929 2.510139e-05 4.626933e-04 7 92536434 92536474 41 + 1.283 0.743 -2.003
ENSG00000127993 E015 39.2247607 0.0025698299 4.940662e-05 8.232319e-04 7 92536475 92536850 376 + 1.606 1.256 -1.209
ENSG00000127993 E016 227.2487904 0.0003160522 1.516009e-09 8.547581e-08 7 92536851 92540481 3631 + 2.268 2.367 0.331