ENSG00000127463

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375199 ENSG00000127463 HEK293_OSMI2_6hA HEK293_TMG_6hB EMC1 protein_coding protein_coding 42.72815 41.69985 46.57494 4.081431 1.48302 0.1594754 4.374258 3.181403 7.172566 0.2308550 0.4710030 1.1703084 0.09914167 0.0767000 0.1541333 0.07743333 0.0004035495 0.0004035495 FALSE TRUE
ENST00000375208 ENSG00000127463 HEK293_OSMI2_6hA HEK293_TMG_6hB EMC1 protein_coding protein_coding 42.72815 41.69985 46.57494 4.081431 1.48302 0.1594754 14.684567 17.492404 10.050857 0.9671998 0.8697436 -0.7987999 0.35941667 0.4284000 0.2151667 -0.21323333 0.0227202779 0.0004035495 FALSE TRUE
ENST00000477853 ENSG00000127463 HEK293_OSMI2_6hA HEK293_TMG_6hB EMC1 protein_coding protein_coding 42.72815 41.69985 46.57494 4.081431 1.48302 0.1594754 5.747926 3.877495 5.941284 0.2450478 0.3659708 0.6143603 0.13858750 0.0954000 0.1274000 0.03200000 0.5833521102 0.0004035495 FALSE TRUE
ENST00000486405 ENSG00000127463 HEK293_OSMI2_6hA HEK293_TMG_6hB EMC1 protein_coding protein_coding 42.72815 41.69985 46.57494 4.081431 1.48302 0.1594754 10.764181 10.457833 16.325739 1.6111168 0.8276855 0.6420689 0.23710417 0.2485667 0.3503667 0.10180000 0.1312378547 0.0004035495 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000127463 E001 183.3895323 0.0034864920 7.171108e-02 2.282481e-01 1 19215660 19216804 1145 - 2.298 2.230 -0.226
ENSG00000127463 E002 33.2868401 0.0180652478 8.956741e-01 9.495607e-01 1 19216805 19216873 69 - 1.528 1.538 0.037
ENSG00000127463 E003 356.7682775 0.0002082912 2.392788e-27 1.556898e-24 1 19216874 19218147 1274 - 2.652 2.442 -0.699
ENSG00000127463 E004 426.6374152 0.0013252170 1.584488e-02 8.122754e-02 1 19218148 19218944 797 - 2.657 2.601 -0.186
ENSG00000127463 E005 644.7791853 0.0011447494 1.430133e-06 3.882185e-05 1 19218945 19219302 358 - 2.749 2.851 0.342
ENSG00000127463 E006 445.1937939 0.0002707160 1.211303e-06 3.353008e-05 1 19219303 19219381 79 - 2.595 2.686 0.300
ENSG00000127463 E007 425.0455524 0.0001868840 2.111001e-05 4.001077e-04 1 19219382 19219482 101 - 2.583 2.661 0.259
ENSG00000127463 E008 285.1119396 0.0002236822 1.730742e-05 3.378776e-04 1 19219569 19219614 46 - 2.399 2.494 0.317
ENSG00000127463 E009 308.3791311 0.0006327207 2.365941e-04 3.073924e-03 1 19219615 19219669 55 - 2.438 2.525 0.291
ENSG00000127463 E010 248.1300034 0.0013149607 4.697527e-02 1.725010e-01 1 19219670 19219698 29 - 2.360 2.418 0.194
ENSG00000127463 E011 19.9646205 0.0644166979 6.064598e-01 7.745235e-01 1 19219699 19220763 1065 - 1.244 1.374 0.455
ENSG00000127463 E012 293.8949482 0.0014475365 9.534201e-02 2.735704e-01 1 19220764 19220810 47 - 2.440 2.488 0.158
ENSG00000127463 E013 264.2882550 0.0006916592 3.682305e-02 1.464497e-01 1 19220811 19220848 38 - 2.389 2.443 0.179
ENSG00000127463 E014 35.9560328 0.0400813768 9.763669e-01 9.899540e-01 1 19220849 19221868 1020 - 1.547 1.577 0.103
ENSG00000127463 E015 26.0875255 0.0648822545 7.744515e-01 8.813937e-01 1 19221869 19222623 755 - 1.423 1.434 0.037
ENSG00000127463 E016 450.1953520 0.0007947696 2.183053e-03 1.829333e-02 1 19222624 19222834 211 - 2.615 2.681 0.221
ENSG00000127463 E017 28.2946546 0.0118072791 1.221727e-01 3.192852e-01 1 19222835 19223395 561 - 1.521 1.403 -0.404
ENSG00000127463 E018 367.9248133 0.0002416248 2.097585e-02 9.927488e-02 1 19223396 19223569 174 - 2.538 2.585 0.154
ENSG00000127463 E019 340.7687474 0.0027754722 2.059315e-01 4.369631e-01 1 19227313 19227450 138 - 2.552 2.515 -0.123
ENSG00000127463 E020 1.3780426 0.6996273546 6.596689e-01   1 19227451 19228190 740 - 0.446 0.320 -0.717
ENSG00000127463 E021 0.8737109 0.0217827725 5.534150e-01   1 19229355 19229554 200 - 0.309 0.205 -0.781
ENSG00000127463 E022 321.1888014 0.0008826531 9.887196e-02 2.798176e-01 1 19230844 19230963 120 - 2.525 2.489 -0.122
ENSG00000127463 E023 5.5684723 0.0154676192 6.761098e-01 8.202471e-01 1 19230964 19231260 297 - 0.838 0.787 -0.199
ENSG00000127463 E024 331.2399208 0.0001605756 1.307389e-03 1.221792e-02 1 19231261 19231422 162 - 2.550 2.488 -0.208
ENSG00000127463 E025 7.1932058 0.0649285729 7.641728e-01 8.751668e-01 1 19231423 19232316 894 - 0.915 0.905 -0.038
ENSG00000127463 E026 308.8046782 0.0019838175 1.993066e-02 9.564500e-02 1 19232624 19232773 150 - 2.519 2.458 -0.206
ENSG00000127463 E027 363.8454150 0.0003248485 8.380618e-01 9.183487e-01 1 19232936 19233135 200 - 2.559 2.557 -0.009
ENSG00000127463 E028 1.1653746 0.0540086340 8.811706e-01   1 19234180 19234188 9 - 0.310 0.344 0.212
ENSG00000127463 E029 0.1779838 0.0335997748 4.312397e-01   1 19234189 19234308 120 - 0.129 0.000 -9.706
ENSG00000127463 E030 272.1074609 0.0011308930 1.980650e-01 4.272158e-01 1 19235130 19235252 123 - 2.411 2.448 0.125
ENSG00000127463 E031 271.9565480 0.0002312043 7.554634e-01 8.699148e-01 1 19237142 19237238 97 - 2.435 2.430 -0.017
ENSG00000127463 E032 293.2961352 0.0014785362 7.875896e-01 8.891352e-01 1 19238017 19238139 123 - 2.461 2.471 0.034
ENSG00000127463 E033 197.9243593 0.0002681877 6.693686e-01 8.158395e-01 1 19238795 19238854 60 - 2.288 2.300 0.041
ENSG00000127463 E034 106.0507277 0.0003985672 9.745753e-01 9.891247e-01 1 19238855 19238857 3 - 2.024 2.026 0.007
ENSG00000127463 E035 199.0360188 0.0002926566 5.187293e-01 7.141933e-01 1 19239231 19239302 72 - 2.288 2.306 0.059
ENSG00000127463 E036 12.0841846 0.0516336211 5.286782e-01 7.210659e-01 1 19239303 19239668 366 - 1.142 1.087 -0.199
ENSG00000127463 E037 9.8738358 0.0777756243 5.610576e-01 7.440431e-01 1 19239669 19239817 149 - 1.056 1.016 -0.146
ENSG00000127463 E038 253.5118812 0.0300184862 3.353593e-01 5.738927e-01 1 19239818 19239985 168 - 2.364 2.437 0.241
ENSG00000127463 E039 232.6029353 1.5137865272 5.920216e-01 7.650368e-01 1 19240297 19240446 150 - 2.309 2.411 0.339
ENSG00000127463 E040 0.0000000       1 19240447 19240568 122 -      
ENSG00000127463 E041 199.1870306 0.0721593182 5.039832e-01 7.035820e-01 1 19241016 19241095 80 - 2.270 2.325 0.184
ENSG00000127463 E042 157.6177248 0.0136873247 2.216001e-01 4.553934e-01 1 19241096 19241136 41 - 2.158 2.231 0.243
ENSG00000127463 E043 132.4897841 0.0110855363 5.507146e-01 7.368447e-01 1 19241137 19241142 6 - 2.105 2.140 0.119
ENSG00000127463 E044 2.3950532 0.2027909457 6.058972e-01 7.741210e-01 1 19241511 19242344 834 - 0.584 0.453 -0.627
ENSG00000127463 E045 189.3721868 0.0021588193 7.081101e-01 8.405800e-01 1 19242345 19242385 41 - 2.284 2.274 -0.036
ENSG00000127463 E046 233.9424429 0.0002984170 3.044684e-02 1.286871e-01 1 19242386 19242473 88 - 2.397 2.346 -0.168
ENSG00000127463 E047 1.9088848 0.0421811550 1.700663e-01 3.905342e-01 1 19243418 19243515 98 - 0.309 0.569 1.384
ENSG00000127463 E048 138.0499506 0.0028554195 6.172854e-03 4.057217e-02 1 19243614 19243619 6 - 2.192 2.091 -0.337
ENSG00000127463 E049 296.6506494 0.0015357635 3.086604e-05 5.535833e-04 1 19243620 19243707 88 - 2.526 2.417 -0.364
ENSG00000127463 E050 1.8920293 0.3140396233 5.363450e-01 7.265424e-01 1 19243708 19243949 242 - 0.543 0.386 -0.802
ENSG00000127463 E051 268.3897502 0.0032500877 1.733550e-04 2.373210e-03 1 19243950 19244015 66 - 2.491 2.368 -0.411
ENSG00000127463 E052 2.2407222 0.0350994528 7.813869e-01 8.854787e-01 1 19244016 19244738 723 - 0.535 0.492 -0.206
ENSG00000127463 E053 1.4104930 0.3057128096 3.654434e-01 6.002930e-01 1 19244739 19244905 167 - 0.480 0.283 -1.135
ENSG00000127463 E054 304.7448364 0.0027619045 9.771612e-06 2.055044e-04 1 19244906 19245030 125 - 2.552 2.417 -0.449
ENSG00000127463 E055 170.9385520 0.0003625591 2.955707e-04 3.684826e-03 1 19251415 19251552 138 - 2.284 2.185 -0.333