ENSG00000127415

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000247933 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding protein_coding 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.3077492 0.3654704 0.02555484 0.1090277 0.02555484 -3.4005813 0.07145417 0.06930000 0.01476667 -0.054533333 0.5546037335 0.0003495542 FALSE TRUE
ENST00000506561 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding retained_intron 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 1.1974967 2.1101457 0.44945816 0.8352596 0.05137095 -2.2061580 0.23702083 0.28046667 0.26823333 -0.012233333 0.9936870066 0.0003495542 FALSE TRUE
ENST00000509744 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding processed_transcript 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.3663602 0.5484149 0.16529429 0.1560151 0.09845666 -1.6715585 0.09515000 0.09090000 0.09286667 0.001966667 1.0000000000 0.0003495542   FALSE
ENST00000509948 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding processed_transcript 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.4106165 0.8370078 0.15328213 0.1320746 0.08330687 -2.3750084 0.09620000 0.14363333 0.09346667 -0.050166667 0.8636666566 0.0003495542 FALSE FALSE
ENST00000514417 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding retained_intron 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.7809363 0.5643897 0.41693172 0.2997145 0.20848439 -0.4280246 0.17771667 0.07923333 0.22770000 0.148466667 0.8320924286 0.0003495542   FALSE
ENST00000514698 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding retained_intron 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.3625531 0.6894867 0.04978152 0.3673504 0.03481284 -3.5485251 0.06732917 0.08266667 0.02626667 -0.056400000 0.4125193240 0.0003495542 TRUE TRUE
ENST00000652070 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding processed_transcript 4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.2995112 0.8904777 0.46440842 0.5485861 0.06804145 -0.9245609 0.08430000 0.09910000 0.27673333 0.177633333 0.5626203035 0.0003495542 FALSE TRUE
MSTRG.24432.13 ENSG00000127415 HEK293_OSMI2_6hA HEK293_TMG_6hB IDUA protein_coding   4.629698 7.290437 1.724711 2.340109 0.1302402 -2.073287 0.5511044 0.7741514 0.00000000 0.2018369 0.00000000 -6.2930603 0.09784583 0.11186667 0.00000000 -0.111866667 0.0003495542 0.0003495542 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000127415 E001 0.6839606 0.020647655 0.501270201   4 986997 987043 47 + 0.259 0.141 -1.090
ENSG00000127415 E002 0.6839606 0.020647655 0.501270201   4 987044 987065 22 + 0.259 0.141 -1.090
ENSG00000127415 E003 0.6839606 0.020647655 0.501270201   4 987066 987075 10 + 0.259 0.141 -1.090
ENSG00000127415 E004 0.6839606 0.020647655 0.501270201   4 987076 987086 11 + 0.259 0.141 -1.090
ENSG00000127415 E005 5.0921815 0.023620289 0.532051335 0.72348467 4 987087 987242 156 + 0.538 0.661 0.548
ENSG00000127415 E006 10.9244269 0.037708050 0.659279927 0.80953623 4 987809 987911 103 + 0.874 0.934 0.227
ENSG00000127415 E007 6.1961375 0.044074200 0.597288788 0.76851717 4 987912 987914 3 + 0.629 0.722 0.392
ENSG00000127415 E008 8.4749539 0.067049804 0.630602280 0.79081334 4 987915 987949 35 + 0.767 0.835 0.268
ENSG00000127415 E009 4.4762808 0.007519247 0.201702356 0.43175835 4 989231 989623 393 + 0.770 0.567 -0.863
ENSG00000127415 E010 2.2969772 0.009789884 0.483623579 0.68951674 4 991825 991871 47 + 0.259 0.425 1.026
ENSG00000127415 E011 2.8846812 0.008649433 0.298133694 0.53861085 4 991872 991940 69 + 0.259 0.501 1.413
ENSG00000127415 E012 7.0755778 0.037127788 0.999973953 1.00000000 4 991941 992230 290 + 0.770 0.759 -0.045
ENSG00000127415 E013 1.0717634 0.020736231 0.294526237   4 999710 999741 32 + 0.420 0.214 -1.356
ENSG00000127415 E014 5.6526867 0.007250456 0.014753733 0.07715237 4 999742 1000064 323 + 0.962 0.610 -1.410
ENSG00000127415 E015 20.5762585 0.006259177 0.132569202 0.33586400 4 1000612 1000697 86 + 1.296 1.158 -0.486
ENSG00000127415 E016 22.0968223 0.003116607 0.574496114 0.75333396 4 1000882 1000989 108 + 1.238 1.199 -0.139
ENSG00000127415 E017 9.3567054 0.082302524 0.653998511 0.80610830 4 1000990 1001179 190 + 0.706 0.880 0.691
ENSG00000127415 E018 19.2826494 0.002582740 0.685310946 0.82607489 4 1001468 1001563 96 + 1.172 1.147 -0.090
ENSG00000127415 E019 25.1703815 0.015482669 0.418977043 0.64288498 4 1001679 1001881 203 + 1.295 1.253 -0.146
ENSG00000127415 E020 6.3882217 0.733629588 0.887246894 0.94522154 4 1001882 1001981 100 + 0.632 0.740 0.451
ENSG00000127415 E021 22.8848851 0.045490267 0.801574581 0.89723498 4 1001982 1002161 180 + 1.173 1.224 0.182
ENSG00000127415 E022 3.9680683 0.006764080 0.143883897 0.35322137 4 1002162 1002268 107 + 0.259 0.595 1.850
ENSG00000127415 E023 22.2953103 0.040045302 0.573182099 0.75244661 4 1002269 1002485 217 + 1.065 1.222 0.564
ENSG00000127415 E024 6.3689455 0.052956119 0.836917478 0.91772349 4 1002732 1002740 9 + 0.629 0.734 0.440
ENSG00000127415 E025 13.7936435 0.002682454 0.162951206 0.38114793 4 1002741 1002944 204 + 0.826 1.040 0.804
ENSG00000127415 E026 7.3229494 0.017389837 0.009772053 0.05713615 4 1003036 1003157 122 + 0.259 0.827 2.811
ENSG00000127415 E027 2.6111784 0.008903279 0.068586258 0.22189141 4 1003158 1003344 187 + 0.000 0.483 11.752
ENSG00000127415 E028 3.3917555 0.008950428 0.034404602 0.13997827 4 1003345 1003412 68 + 0.000 0.551 12.023
ENSG00000127415 E029 3.3917555 0.008950428 0.034404602 0.13997827 4 1003413 1003428 16 + 0.000 0.551 12.023
ENSG00000127415 E030 6.7420904 0.004803943 0.868563984 0.93512696 4 1003429 1003470 42 + 0.705 0.748 0.175
ENSG00000127415 E031 11.3399596 0.003182563 0.193499228 0.42146834 4 1003549 1003625 77 + 1.064 0.918 -0.541
ENSG00000127415 E032 1.8325793 0.018274642 0.225069820 0.45951449 4 1003626 1003629 4 + 0.538 0.306 -1.262
ENSG00000127415 E033 2.3901111 0.010438178 0.159809409 0.37651133 4 1003630 1003724 95 + 0.630 0.381 -1.218
ENSG00000127415 E034 20.3040932 0.002185465 0.214728673 0.44720350 4 1004012 1004112 101 + 1.258 1.151 -0.379
ENSG00000127415 E035 22.8364129 0.001697175 0.419660543 0.64341306 4 1004260 1004564 305 + 1.122 1.227 0.374