ENSG00000127334

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000319833 ENSG00000127334 HEK293_OSMI2_6hA HEK293_TMG_6hB DYRK2 protein_coding protein_coding 5.875766 2.839583 8.743068 0.5555136 0.2877813 1.619038 0.4324096 0.0000000 2.115594 0.0000000 0.46567758 7.7317222 0.06520833 0.0000000 0.2411000 0.2411000 1.537610e-07 1.53761e-07 FALSE TRUE
ENST00000344096 ENSG00000127334 HEK293_OSMI2_6hA HEK293_TMG_6hB DYRK2 protein_coding protein_coding 5.875766 2.839583 8.743068 0.5555136 0.2877813 1.619038 3.7984261 2.1377348 4.017551 0.4232923 0.09121437 0.9070870 0.65800417 0.7544333 0.4608000 -0.2936333 5.237646e-03 1.53761e-07 FALSE TRUE
ENST00000393555 ENSG00000127334 HEK293_OSMI2_6hA HEK293_TMG_6hB DYRK2 protein_coding protein_coding 5.875766 2.839583 8.743068 0.5555136 0.2877813 1.619038 1.3345250 0.5778516 1.010170 0.2372921 0.28584369 0.7952862 0.23008750 0.2041000 0.1180000 -0.0861000 7.342507e-01 1.53761e-07 FALSE TRUE
ENST00000543747 ENSG00000127334 HEK293_OSMI2_6hA HEK293_TMG_6hB DYRK2 protein_coding protein_coding 5.875766 2.839583 8.743068 0.5555136 0.2877813 1.619038 0.1295211 0.0000000 1.002144 0.0000000 1.00214409 6.6612709 0.01432083 0.0000000 0.1110333 0.1110333 1.000000e+00 1.53761e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000127334 E001 0.1308682 0.032649190 2.140317e-01   12 67648338 67648414 77 + 0.000 0.136 10.235
ENSG00000127334 E002 6.6950886 0.365770856 6.129414e-01 7.789185e-01 12 67648745 67648777 33 + 0.934 0.825 -0.415
ENSG00000127334 E003 22.2627520 0.496877716 1.262936e-01 3.259509e-01 12 67648778 67649120 343 + 1.292 1.442 0.521
ENSG00000127334 E004 14.7120054 0.118458197 1.668191e-05 3.273598e-04 12 67649121 67649182 62 + 0.882 1.416 1.919
ENSG00000127334 E005 0.4279040 0.595272510 5.870477e-01   12 67649682 67649796 115 + 0.259 0.000 -11.055
ENSG00000127334 E006 31.4575156 0.001827583 2.976918e-08 1.253842e-06 12 67649797 67649945 149 + 1.459 1.576 0.400
ENSG00000127334 E007 1.8800255 0.011039026 8.593104e-01 9.299695e-01 12 67651469 67651658 190 + 0.514 0.392 -0.629
ENSG00000127334 E008 1.0220253 0.286832789 4.945742e-01   12 67652501 67652700 200 + 0.425 0.134 -2.202
ENSG00000127334 E009 1119.4569814 0.045440950 6.554471e-05 1.049797e-03 12 67657106 67665406 8301 + 3.146 2.875 -0.901