ENSG00000126453

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000246784 ENSG00000126453 HEK293_OSMI2_6hA HEK293_TMG_6hB BCL2L12 protein_coding protein_coding 140.3097 219.7185 68.57308 43.26036 2.136109 -1.679797 83.814463 138.941875 30.924256 28.612412 3.4756663 -2.1673081 0.56767917 0.62920000 0.44873333 -0.18046667 0.02653737 0.02653737 FALSE TRUE
ENST00000594157 ENSG00000126453 HEK293_OSMI2_6hA HEK293_TMG_6hB BCL2L12 protein_coding protein_coding 140.3097 219.7185 68.57308 43.26036 2.136109 -1.679797 11.650610 15.222431 7.710488 4.229287 3.8560968 -0.9803822 0.09278750 0.06640000 0.10926667 0.04286667 1.00000000 0.02653737 FALSE FALSE
ENST00000598979 ENSG00000126453 HEK293_OSMI2_6hA HEK293_TMG_6hB BCL2L12 protein_coding nonsense_mediated_decay 140.3097 219.7185 68.57308 43.26036 2.136109 -1.679797 15.050040 23.259962 8.011392 7.983279 1.9173010 -1.5365444 0.10023333 0.09820000 0.11883333 0.02063333 0.93772310 0.02653737 TRUE FALSE
ENST00000601168 ENSG00000126453 HEK293_OSMI2_6hA HEK293_TMG_6hB BCL2L12 protein_coding retained_intron 140.3097 219.7185 68.57308 43.26036 2.136109 -1.679797 6.764826 9.368706 6.695618 1.645110 0.9363712 -0.4840185 0.06277083 0.05066667 0.09746667 0.04680000 0.43463656 0.02653737   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000126453 E001 2.375813 9.008705e-03 6.319702e-01 0.7916783101 19 49665142 49665565 424 + 0.376 0.472 0.516
ENSG00000126453 E002 3.196986 8.084361e-03 8.290884e-01 0.9133035228 19 49665566 49665932 367 + 0.574 0.534 -0.183
ENSG00000126453 E003 21.617640 9.239040e-03 2.908249e-01 0.5314446931 19 49665933 49665960 28 + 1.148 1.269 0.429
ENSG00000126453 E004 57.697487 1.724786e-02 1.440862e-01 0.3535135089 19 49665961 49665997 37 + 1.531 1.677 0.498
ENSG00000126453 E005 57.435751 1.705601e-02 1.489527e-01 0.3607796611 19 49665998 49665998 1 + 1.531 1.675 0.490
ENSG00000126453 E006 130.488225 1.266954e-02 3.607662e-01 0.5962608305 19 49665999 49666012 14 + 2.047 1.993 -0.180
ENSG00000126453 E007 605.617274 1.527728e-03 6.298675e-01 0.7903696780 19 49666013 49666067 55 + 2.659 2.672 0.043
ENSG00000126453 E008 1035.536303 2.790306e-04 3.558372e-01 0.5923807025 19 49666685 49666799 115 + 2.916 2.901 -0.048
ENSG00000126453 E009 71.940169 1.031028e-01 2.859844e-02 0.1231376593 19 49666800 49667018 219 + 2.021 1.656 -1.230
ENSG00000126453 E010 461.124143 8.386395e-04 2.831558e-01 0.5235962358 19 49667019 49667021 3 + 2.533 2.555 0.072
ENSG00000126453 E011 830.679245 8.484475e-04 4.272570e-01 0.6489309250 19 49667022 49667087 66 + 2.798 2.807 0.031
ENSG00000126453 E012 779.025731 4.623922e-04 3.265601e-01 0.5657591606 19 49667088 49667161 74 + 2.767 2.781 0.048
ENSG00000126453 E013 1017.302940 8.468466e-05 1.984171e-02 0.0953393736 19 49668851 49668937 87 + 2.922 2.891 -0.106
ENSG00000126453 E014 619.026885 2.837442e-04 1.448842e-02 0.0762014098 19 49669024 49669047 24 + 2.718 2.670 -0.158
ENSG00000126453 E015 839.843583 4.867460e-04 3.284168e-02 0.1356043839 19 49669048 49669115 68 + 2.843 2.804 -0.130
ENSG00000126453 E016 23.764301 5.173562e-02 4.062120e-03 0.0295251291 19 49669116 49669164 49 + 1.579 1.177 -1.394
ENSG00000126453 E017 971.341732 7.390459e-04 4.247101e-07 0.0000134312 19 49670216 49670436 221 + 2.794 2.890 0.318
ENSG00000126453 E018 415.714178 4.392549e-04 2.842598e-04 0.0035690876 19 49670437 49670488 52 + 2.435 2.525 0.298
ENSG00000126453 E019 36.599122 8.682735e-02 6.481033e-02 0.2134738475 19 49672109 49672201 93 + 1.693 1.390 -1.037
ENSG00000126453 E020 681.672287 1.774952e-04 2.453245e-01 0.4827324014 19 49673698 49673916 219 + 2.741 2.720 -0.068