ENSG00000125912

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000246117 ENSG00000125912 HEK293_OSMI2_6hA HEK293_TMG_6hB NCLN protein_coding protein_coding 110.0301 163.1808 74.90246 25.46332 1.304224 -1.123282 65.054999 101.029056 40.403704 15.2579089 1.6147399 -1.3219966 0.59282917 0.62006667 0.53916667 -0.08090000 0.15110770 0.03932475 FALSE TRUE
ENST00000587740 ENSG00000125912 HEK293_OSMI2_6hA HEK293_TMG_6hB NCLN protein_coding nonsense_mediated_decay 110.0301 163.1808 74.90246 25.46332 1.304224 -1.123282 2.852157 2.060161 6.557145 0.6318747 2.2049953 1.6655231 0.03188750 0.01436667 0.08736667 0.07300000 0.03932475 0.03932475 TRUE TRUE
ENST00000592737 ENSG00000125912 HEK293_OSMI2_6hA HEK293_TMG_6hB NCLN protein_coding protein_coding 110.0301 163.1808 74.90246 25.46332 1.304224 -1.123282 33.582153 48.834294 20.646701 8.7639252 0.7050807 -1.2415802 0.28387500 0.29656667 0.27613333 -0.02043333 0.86465132 0.03932475 FALSE TRUE
MSTRG.16158.4 ENSG00000125912 HEK293_OSMI2_6hA HEK293_TMG_6hB NCLN protein_coding   110.0301 163.1808 74.90246 25.46332 1.304224 -1.123282 6.006028 8.287310 6.100950 1.7983533 1.2555070 -0.4412469 0.06678333 0.05003333 0.08143333 0.03140000 0.52059634 0.03932475 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000125912 E001 2.048868 2.951542e-01 5.074071e-01 7.059709e-01 19 3185563 3185718 156 + 0.557 0.388 -0.854
ENSG00000125912 E002 3.256301 1.204751e-02 7.248810e-01 8.510440e-01 19 3185719 3185914 196 + 0.625 0.571 -0.241
ENSG00000125912 E003 10.010220 1.714014e-02 7.627260e-04 7.968037e-03 19 3185915 3185929 15 + 0.566 1.096 2.098
ENSG00000125912 E004 12.306720 1.610945e-02 1.692439e-03 1.496875e-02 19 3185930 3185935 6 + 0.723 1.169 1.683
ENSG00000125912 E005 83.329301 1.898178e-02 6.464104e-07 1.943271e-05 19 3185936 3185956 21 + 1.516 1.951 1.475
ENSG00000125912 E006 493.064390 2.538542e-03 7.414097e-06 1.617736e-04 19 3185957 3186214 258 + 2.532 2.672 0.468
ENSG00000125912 E007 712.878859 1.076887e-04 1.393788e-05 2.794537e-04 19 3192470 3192660 191 + 2.745 2.820 0.248
ENSG00000125912 E008 837.856956 1.992265e-03 6.669175e-01 8.142866e-01 19 3193284 3193428 145 + 2.876 2.867 -0.030
ENSG00000125912 E009 752.111730 1.155362e-03 1.449623e-01 3.548796e-01 19 3196183 3196277 95 + 2.844 2.815 -0.098
ENSG00000125912 E010 452.842194 1.633985e-04 6.866668e-01 8.269404e-01 19 3198817 3198840 24 + 2.607 2.605 -0.007
ENSG00000125912 E011 575.717393 1.258973e-04 1.435838e-01 3.527677e-01 19 3198841 3198897 57 + 2.723 2.703 -0.064
ENSG00000125912 E012 728.675173 2.007758e-04 1.886500e-02 9.198663e-02 19 3201523 3201626 104 + 2.833 2.801 -0.108
ENSG00000125912 E013 574.360991 1.864770e-04 2.287418e-02 1.055387e-01 19 3203756 3203796 41 + 2.732 2.697 -0.115
ENSG00000125912 E014 641.615758 2.530837e-04 9.799311e-02 2.782531e-01 19 3203797 3203844 48 + 2.772 2.748 -0.080
ENSG00000125912 E015 448.096361 6.484963e-04 2.447274e-01 4.818815e-01 19 3204005 3204014 10 + 2.615 2.592 -0.078
ENSG00000125912 E016 816.123278 1.145823e-04 3.697436e-01 6.038019e-01 19 3204015 3204144 130 + 2.847 2.866 0.063
ENSG00000125912 E017 1140.174931 1.398520e-04 2.748623e-01 5.148642e-01 19 3204573 3204751 179 + 3.012 3.003 -0.029
ENSG00000125912 E018 9.657198 1.549002e-02 4.405798e-01 6.591388e-01 19 3205832 3205938 107 + 1.048 0.952 -0.355
ENSG00000125912 E019 925.461057 2.698671e-04 2.959899e-07 9.741453e-06 19 3205939 3206026 88 + 2.964 2.893 -0.237
ENSG00000125912 E020 692.016200 1.170746e-04 1.039561e-06 2.938439e-05 19 3206152 3206190 39 + 2.838 2.769 -0.230
ENSG00000125912 E021 228.742129 2.045835e-04 8.329292e-03 5.083395e-02 19 3206262 3206264 3 + 2.356 2.293 -0.210
ENSG00000125912 E022 1173.708177 7.291448e-05 5.714061e-04 6.322099e-03 19 3206265 3206421 157 + 3.043 3.008 -0.115
ENSG00000125912 E023 570.468045 3.347187e-04 7.639123e-02 2.375048e-01 19 3206422 3206425 4 + 2.725 2.698 -0.091
ENSG00000125912 E024 774.361940 1.985936e-04 7.952040e-03 4.909974e-02 19 3207198 3207251 54 + 2.863 2.828 -0.116
ENSG00000125912 E025 788.958523 8.739526e-05 3.584218e-01 5.945292e-01 19 3207391 3207465 75 + 2.851 2.844 -0.024
ENSG00000125912 E026 494.912700 4.442369e-04 3.148844e-01 5.550268e-01 19 3207466 3207469 4 + 2.654 2.638 -0.055
ENSG00000125912 E027 1.415037 2.260537e-01 6.847060e-01 8.257560e-01 19 3207470 3207490 21 + 0.422 0.299 -0.727
ENSG00000125912 E028 914.082578 7.538001e-04 9.133576e-01 9.587658e-01 19 3207629 3207747 119 + 2.907 2.907 -0.001
ENSG00000125912 E029 4541.967880 8.544243e-05 4.375308e-13 4.806132e-11 19 3207748 3209234 1487 + 3.566 3.617 0.170
ENSG00000125912 E030 1307.696291 1.600884e-03 1.190457e-01 3.143094e-01 19 3209235 3209477 243 + 3.033 3.071 0.128
ENSG00000125912 E031 289.481281 1.176412e-03 6.878806e-01 8.277422e-01 19 3209478 3209575 98 + 2.397 2.415 0.059