ENSG00000125814

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377026 ENSG00000125814 HEK293_OSMI2_6hA HEK293_TMG_6hB NAPB protein_coding protein_coding 4.161378 2.429453 5.474122 0.3313586 0.1488484 1.168703 0.8723207 0.0000000 2.0713136 0.00000000 0.61405578 7.7013505 0.2116750 0.00000000 0.3835000 0.38350000 5.033543e-05 5.033543e-05 FALSE TRUE
ENST00000398425 ENSG00000125814 HEK293_OSMI2_6hA HEK293_TMG_6hB NAPB protein_coding protein_coding 4.161378 2.429453 5.474122 0.3313586 0.1488484 1.168703 0.8890466 0.8412030 1.5499236 0.47116897 0.11999703 0.8739003 0.2063875 0.30553333 0.2823333 -0.02320000 9.800349e-01 5.033543e-05 FALSE TRUE
ENST00000432543 ENSG00000125814 HEK293_OSMI2_6hA HEK293_TMG_6hB NAPB protein_coding protein_coding 4.161378 2.429453 5.474122 0.3313586 0.1488484 1.168703 1.4045192 1.0450340 0.6423685 0.14475469 0.64236847 -0.6935305 0.3643917 0.46280000 0.1125333 -0.35026667 2.320092e-01 5.033543e-05 FALSE TRUE
MSTRG.20481.1 ENSG00000125814 HEK293_OSMI2_6hA HEK293_TMG_6hB NAPB protein_coding   4.161378 2.429453 5.474122 0.3313586 0.1488484 1.168703 0.3453533 0.1077845 0.4994049 0.02440623 0.02091875 2.1126633 0.0731375 0.04353333 0.0915000 0.04796667 4.667998e-01 5.033543e-05 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000125814 E001 3.5178309 0.0068118776 0.488759352 0.69316624 20 23373817 23374055 239 - 0.673 0.567 -0.462
ENSG00000125814 E002 0.8267829 0.0181189406 0.796521881   20 23374519 23374521 3 - 0.229 0.278 0.369
ENSG00000125814 E003 146.5509570 0.0003597553 0.003451580 0.02605377 20 23374522 23376072 1551 - 2.113 2.192 0.264
ENSG00000125814 E004 101.4903488 0.0067908947 0.549435559 0.73592234 20 23376073 23377031 959 - 1.975 2.007 0.107
ENSG00000125814 E005 52.7479483 0.0054745361 0.701944665 0.83680003 20 23377032 23377486 455 - 1.699 1.725 0.086
ENSG00000125814 E006 2.6667750 0.0089267066 0.427890931 0.64940410 20 23378799 23379444 646 - 0.578 0.447 -0.631
ENSG00000125814 E007 18.4759698 0.0144229023 0.307678973 0.54834615 20 23379445 23379471 27 - 1.230 1.328 0.346
ENSG00000125814 E008 14.9215723 0.0164766607 0.382712734 0.61447452 20 23379472 23379495 24 - 1.145 1.237 0.329
ENSG00000125814 E009 2.7272345 0.0128876210 0.447596661 0.66400706 20 23379496 23379866 371 - 0.490 0.619 0.596
ENSG00000125814 E010 15.9265788 0.0139818247 0.364953228 0.59990564 20 23379867 23379935 69 - 1.168 1.259 0.321
ENSG00000125814 E011 19.8703480 0.0023300859 0.126753159 0.32663048 20 23381213 23381317 105 - 1.335 1.212 -0.434
ENSG00000125814 E012 15.8619089 0.0040475202 0.010258208 0.05922785 20 23389946 23390030 85 - 1.280 1.036 -0.873
ENSG00000125814 E013 15.7453047 0.0022308624 0.007278685 0.04596960 20 23390209 23390264 56 - 1.270 1.018 -0.904
ENSG00000125814 E014 17.7315949 0.0035367785 0.107470262 0.29482431 20 23394922 23394999 78 - 1.287 1.147 -0.494
ENSG00000125814 E015 7.3836374 0.0085029421 0.018264135 0.08990348 20 23395139 23395185 47 - 0.982 0.662 -1.261
ENSG00000125814 E016 4.6300732 0.0062062511 0.058075891 0.19846306 20 23395186 23395262 77 - 0.802 0.511 -1.249
ENSG00000125814 E017 2.4700101 0.0093125183 0.531363725 0.72299005 20 23396738 23397071 334 - 0.550 0.447 -0.505
ENSG00000125814 E018 10.6971702 0.0424310488 0.067274094 0.21898962 20 23397072 23397188 117 - 1.110 0.868 -0.899
ENSG00000125814 E019 0.6479912 0.0303495721 0.007549421   20 23397189 23397200 12 - 0.000 0.447 11.452
ENSG00000125814 E020 13.5631642 0.0029232756 0.956444410 0.98009947 20 23402993 23403072 80 - 1.138 1.147 0.031
ENSG00000125814 E021 11.5226035 0.0037829356 0.419470177 0.64324644 20 23421305 23421519 215 - 1.100 1.019 -0.297