ENSG00000124226

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000244061 ENSG00000124226 HEK293_OSMI2_6hA HEK293_TMG_6hB RNF114 protein_coding protein_coding 52.41601 54.56028 50.83762 7.81706 2.332502 -0.1019351 34.50080 28.28821 37.271439 2.773361 3.0860217 0.3977465 0.6662125 0.5276667 0.7308667 0.2032 0.03881216 0.01242471 FALSE TRUE
ENST00000625177 ENSG00000124226 HEK293_OSMI2_6hA HEK293_TMG_6hB RNF114 protein_coding protein_coding 52.41601 54.56028 50.83762 7.81706 2.332502 -0.1019351 13.17389 21.98886 8.099759 5.559355 0.6102826 -1.4396977 0.2393917 0.3877000 0.1603000 -0.2274 0.01242471 0.01242471 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000124226 E001 0.9997096 0.2234118516 1.790762e-01   20 49936336 49936376 41 + 0.135 0.420 2.161
ENSG00000124226 E002 11.0214693 0.0032448088 3.606516e-04 4.343436e-03 20 49936377 49936396 20 + 0.860 1.189 1.209
ENSG00000124226 E003 11.7122071 0.0028315448 5.851141e-04 6.448977e-03 20 49936397 49936398 2 + 0.902 1.205 1.106
ENSG00000124226 E004 22.9636070 0.0072106022 1.270614e-01 3.271311e-01 20 49936399 49936406 8 + 1.334 1.408 0.256
ENSG00000124226 E005 251.7080065 0.0025237742 2.939455e-04 3.668874e-03 20 49936407 49936552 146 + 2.361 2.424 0.212
ENSG00000124226 E006 2.0806619 0.2620192213 6.546500e-01 8.064841e-01 20 49941107 49941220 114 + 0.550 0.434 -0.567
ENSG00000124226 E007 173.5061596 0.0015695376 1.685548e-05 3.301173e-04 20 49941561 49941582 22 + 2.187 2.272 0.285
ENSG00000124226 E008 400.3314875 0.0006596756 1.496839e-06 4.040014e-05 20 49941583 49941711 129 + 2.573 2.616 0.143
ENSG00000124226 E009 10.0275129 0.0543714511 1.622741e-01 3.801716e-01 20 49941712 49941971 260 + 1.173 0.908 -0.970
ENSG00000124226 E010 54.5690424 0.0811624997 1.578952e-01 3.735718e-01 20 49943931 49945381 1451 + 1.856 1.625 -0.783
ENSG00000124226 E011 475.1229050 0.0003041869 5.066494e-05 8.414520e-04 20 49945382 49945488 107 + 2.664 2.677 0.044
ENSG00000124226 E012 456.3053424 0.0018841183 3.795668e-01 6.118516e-01 20 49946136 49946250 115 + 2.674 2.642 -0.107
ENSG00000124226 E013 1.1959221 0.4724949404 6.211888e-01   20 49946251 49946254 4 + 0.399 0.272 -0.790
ENSG00000124226 E014 414.2146834 0.0026910981 2.075687e-01 4.388928e-01 20 49949248 49949355 108 + 2.624 2.606 -0.061
ENSG00000124226 E015 283.3067681 0.0002137787 7.875180e-01 8.890825e-01 20 49952076 49952138 63 + 2.477 2.427 -0.168
ENSG00000124226 E016 1402.6751570 0.0078317479 3.553383e-04 4.289919e-03 20 49952139 49953885 1747 + 3.228 3.066 -0.539