ENSG00000124198

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371917 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding protein_coding 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 2.3624489 0.89170603 2.64584248 0.31386777 0.44297992 1.5584405 0.33041667 0.31763333 0.24650000 -0.07113333 0.8610826629 0.0001664707 FALSE TRUE
ENST00000679436 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding protein_coding 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 1.7960111 0.85583404 3.73588856 0.28868670 0.42631918 2.1131456 0.27167917 0.32003333 0.35610000 0.03606667 0.9436008289 0.0001664707 FALSE TRUE
ENST00000679542 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding retained_intron 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 0.2597929 0.00000000 0.79689397 0.00000000 0.11949047 6.3343072 0.02822917 0.00000000 0.07416667 0.07416667 0.0001664707 0.0001664707 TRUE TRUE
ENST00000679747 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding retained_intron 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 0.2048254 0.16833110 0.18527963 0.04077444 0.02948339 0.1309831 0.04996250 0.07366667 0.01743333 -0.05623333 0.2039860762 0.0001664707 FALSE TRUE
ENST00000681119 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding retained_intron 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 0.1900947 0.49958807 0.05137022 0.17035153 0.05137022 -3.0537208 0.07105833 0.17293333 0.00440000 -0.16853333 0.0006887634 0.0001664707 FALSE TRUE
ENST00000681656 ENSG00000124198 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF2 protein_coding nonsense_mediated_decay 6.241973 2.687117 10.64988 0.4525735 0.5147857 1.982701 0.4486521 0.06362159 1.18283265 0.06362159 0.15927877 4.0181190 0.07150417 0.02103333 0.11020000 0.08916667 0.1897001145 0.0001664707 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000124198 E001 2.9167448 0.0098434442 1.009300e-01 2.834689e-01 20 48921711 48921828 118 + 0.456 0.726 1.217
ENSG00000124198 E002 4.8694442 0.0054087281 2.827611e-01 5.231988e-01 20 48921829 48921889 61 + 0.657 0.817 0.648
ENSG00000124198 E003 14.5643040 0.0373952385 5.573705e-01 7.413779e-01 20 48921890 48922010 121 + 1.129 1.037 -0.332
ENSG00000124198 E004 11.7137473 0.0075891112 2.055492e-01 4.365361e-01 20 48941199 48941229 31 + 1.054 0.891 -0.603
ENSG00000124198 E005 22.7951397 0.0262575557 3.689225e-01 6.031493e-01 20 48941864 48941987 124 + 1.318 1.200 -0.416
ENSG00000124198 E006 31.3743725 0.0338597487 1.987080e-01 4.280484e-01 20 48951323 48951469 147 + 1.459 1.295 -0.567
ENSG00000124198 E007 14.6330433 0.0746240527 2.490165e-01 4.868047e-01 20 48952705 48952724 20 + 1.147 0.967 -0.657
ENSG00000124198 E008 36.2762714 0.0064263500 1.013342e-01 2.842470e-01 20 48952725 48952884 160 + 1.516 1.378 -0.475
ENSG00000124198 E009 52.9656580 0.0038809734 3.729263e-02 1.477119e-01 20 48953556 48953790 235 + 1.683 1.542 -0.479
ENSG00000124198 E010 32.6094589 0.0024151725 1.592118e-02 8.149567e-02 20 48963830 48963898 69 + 1.487 1.290 -0.682
ENSG00000124198 E011 15.8629788 0.0168624556 3.967649e-01 6.257654e-01 20 48965872 48965874 3 + 1.179 1.080 -0.357
ENSG00000124198 E012 21.7484453 0.0076079481 1.814385e-01 4.058897e-01 20 48965875 48965877 3 + 1.312 1.180 -0.467
ENSG00000124198 E013 46.3060560 0.0029196634 2.205053e-02 1.027518e-01 20 48965878 48966023 146 + 1.628 1.467 -0.550
ENSG00000124198 E014 36.9458825 0.0077442345 9.102140e-02 2.655416e-01 20 48969147 48969277 131 + 1.523 1.377 -0.502
ENSG00000124198 E015 49.9518925 0.0007318384 8.210952e-03 5.030414e-02 20 48971120 48971354 235 + 1.657 1.486 -0.583
ENSG00000124198 E016 33.5451883 0.0013026615 2.414044e-02 1.094979e-01 20 48972326 48972425 100 + 1.494 1.317 -0.612
ENSG00000124198 E017 41.6632744 0.0009029836 2.882473e-03 2.269954e-02 20 48973145 48973284 140 + 1.590 1.377 -0.733
ENSG00000124198 E018 38.3213503 0.0038213257 3.558383e-03 2.669739e-02 20 48974766 48974874 109 + 1.559 1.328 -0.796
ENSG00000124198 E019 50.2811548 0.0007788910 2.560089e-03 2.072170e-02 20 48976016 48976199 184 + 1.665 1.468 -0.671
ENSG00000124198 E020 37.8655704 0.0017033575 3.725356e-02 1.476225e-01 20 48984729 48984840 112 + 1.534 1.378 -0.540
ENSG00000124198 E021 53.4615385 0.0244465193 9.666794e-02 2.759282e-01 20 48985408 48985613 206 + 1.682 1.527 -0.528
ENSG00000124198 E022 38.8433669 0.0049088868 8.295479e-02 2.506517e-01 20 48988304 48988388 85 + 1.545 1.410 -0.465
ENSG00000124198 E023 44.7077510 0.0008146711 1.013318e-02 5.870995e-02 20 48988491 48988662 172 + 1.614 1.440 -0.598
ENSG00000124198 E024 39.2079868 0.0039360501 2.995541e-02 1.272193e-01 20 48989285 48989436 152 + 1.556 1.388 -0.579
ENSG00000124198 E025 43.7511380 0.0010713492 1.605818e-01 3.776432e-01 20 48989556 48989684 129 + 1.589 1.495 -0.322
ENSG00000124198 E026 62.7343867 0.0006805907 1.810722e-02 8.939019e-02 20 48991040 48991198 159 + 1.748 1.614 -0.456
ENSG00000124198 E027 58.6572619 0.0031092340 4.430249e-01 6.607633e-01 20 48994451 48994598 148 + 1.704 1.657 -0.161
ENSG00000124198 E028 47.7917030 0.0008735120 8.033655e-01 8.982879e-01 20 48995783 48995882 100 + 1.611 1.594 -0.056
ENSG00000124198 E029 0.3040503 0.0274424043 1.000000e+00   20 48998100 48998192 93 + 0.126 0.000 -9.226
ENSG00000124198 E030 31.4105595 0.0016958321 8.833152e-01 9.430063e-01 20 48998193 48998233 41 + 1.431 1.440 0.032
ENSG00000124198 E031 50.5362740 0.0043844540 5.462134e-01 7.336080e-01 20 48998336 48998505 170 + 1.643 1.600 -0.145
ENSG00000124198 E032 40.7061035 0.0010319055 2.443157e-01 4.814423e-01 20 49005070 49005221 152 + 1.557 1.477 -0.274
ENSG00000124198 E033 45.3163768 0.0016323027 1.325323e-01 3.358006e-01 20 49010232 49010404 173 + 1.565 1.657 0.313
ENSG00000124198 E034 56.7697456 0.0007928544 3.393785e-01 5.774136e-01 20 49011924 49012084 161 + 1.671 1.723 0.176
ENSG00000124198 E035 0.4842470 0.7718991298 1.000000e+00   20 49013048 49013563 516 + 0.179 0.001 -8.538
ENSG00000124198 E036 49.2818073 0.0008239404 9.975564e-01 1.000000e+00 20 49013564 49013694 131 + 1.621 1.620 -0.003
ENSG00000124198 E037 3.8379019 0.0063883644 9.398841e-01 9.721709e-01 20 49013695 49013830 136 + 0.624 0.611 -0.056
ENSG00000124198 E038 48.7836720 0.0021413528 8.086821e-01 9.014001e-01 20 49013831 49013960 130 + 1.616 1.632 0.056
ENSG00000124198 E039 50.8667037 0.0013475769 3.680123e-01 6.024209e-01 20 49016280 49016415 136 + 1.621 1.675 0.181
ENSG00000124198 E040 54.0475962 0.0027978412 3.070459e-01 5.476448e-01 20 49017249 49017387 139 + 1.644 1.707 0.212
ENSG00000124198 E041 36.7259943 0.0115148890 2.468977e-01 4.845746e-01 20 49017496 49017550 55 + 1.473 1.570 0.334
ENSG00000124198 E042 47.5402822 0.0038360398 8.716570e-02 2.585677e-01 20 49018884 49018998 115 + 1.576 1.685 0.371
ENSG00000124198 E043 1.2673108 0.0129534904 8.115447e-01   20 49018999 49019181 183 + 0.304 0.349 0.285
ENSG00000124198 E044 2.7727051 0.0085770996 4.485374e-01 6.646390e-01 20 49022154 49023050 897 + 0.481 0.611 0.607
ENSG00000124198 E045 54.4766044 0.0043305440 1.447811e-01 3.546365e-01 20 49023051 49023181 131 + 1.636 1.725 0.301
ENSG00000124198 E046 24.7456962 0.0116053328 8.418159e-01 9.204619e-01 20 49025313 49025316 4 + 1.331 1.307 -0.082
ENSG00000124198 E047 65.2726430 0.0013308761 1.347940e-01 3.393690e-01 20 49025317 49025481 165 + 1.717 1.794 0.259
ENSG00000124198 E048 57.5795873 0.0007365064 3.790726e-02 1.493729e-01 20 49028530 49028668 139 + 1.657 1.767 0.372
ENSG00000124198 E049 1.1302857 0.0148075119 6.381165e-01   20 49031315 49032048 734 + 0.304 0.209 -0.717
ENSG00000124198 E050 54.0845018 0.0007620337 9.144248e-02 2.663643e-01 20 49032049 49032166 118 + 1.635 1.728 0.315
ENSG00000124198 E051 8.8220011 0.0991883702 4.461514e-01 6.628358e-01 20 49032167 49033022 856 + 0.879 1.021 0.533
ENSG00000124198 E052 582.8340743 0.0002730375 4.603060e-18 1.075248e-15 20 49033023 49036693 3671 + 2.651 2.784 0.443