ENSG00000124098

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371384 ENSG00000124098 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM210B protein_coding protein_coding 22.60737 16.6855 26.74286 2.62172 1.061786 0.6802343 20.666200 14.306729 25.5170516 2.2526588 0.9420161 0.834325 0.9085375 0.8573667 0.95443333 0.09706667 0.02761378 0.01458742 FALSE TRUE
ENST00000437418 ENSG00000124098 HEK293_OSMI2_6hA HEK293_TMG_6hB FAM210B protein_coding protein_coding 22.60737 16.6855 26.74286 2.62172 1.061786 0.6802343 1.121428 1.408969 0.8290323 0.3516761 0.1424008 -0.758045 0.0514000 0.0815000 0.03073333 -0.05076667 0.01458742 0.01458742   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000124098 E001 10.28922 0.0091560220 3.322383e-08 1.382546e-06 20 56358974 56359108 135 + 0.683 1.309 2.344
ENSG00000124098 E002 31.82203 0.0013143119 1.622861e-03 1.448235e-02 20 56359109 56359191 83 + 1.412 1.610 0.679
ENSG00000124098 E003 13.18909 0.0028923638 1.355220e-05 2.729130e-04 20 56360182 56360712 531 + 0.922 1.335 1.486
ENSG00000124098 E004 114.76167 0.0003754757 4.167467e-01 6.412045e-01 20 56365087 56365262 176 + 2.044 2.073 0.097
ENSG00000124098 E005 154.07691 0.0043574005 9.547778e-01 9.792886e-01 20 56366071 56366194 124 + 2.179 2.186 0.023
ENSG00000124098 E006 469.05304 0.0001999415 2.202851e-07 7.508928e-06 20 56366195 56366966 772 + 2.697 2.621 -0.251
ENSG00000124098 E007 942.32601 0.0005390205 1.288688e-01 3.300035e-01 20 56366967 56368663 1697 + 2.962 2.979 0.057