ENSG00000122965

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261741 ENSG00000122965 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM19 protein_coding protein_coding 26.60934 38.95185 21.60329 5.802447 0.8196147 -0.8501432 9.7233336 14.816319 7.772157 2.8528523 0.6765892 -0.9299184 0.35353333 0.3744000 0.35970000 -0.01470000 9.638416e-01 8.874548e-16 FALSE TRUE
ENST00000392561 ENSG00000122965 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM19 protein_coding protein_coding 26.60934 38.95185 21.60329 5.802447 0.8196147 -0.8501432 12.2897462 18.715673 8.846525 3.3201646 0.2270863 -1.0802050 0.46230833 0.4754667 0.40993333 -0.06553333 4.383860e-01 8.874548e-16 FALSE TRUE
ENST00000545145 ENSG00000122965 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM19 protein_coding protein_coding 26.60934 38.95185 21.60329 5.802447 0.8196147 -0.8501432 2.8389111 4.972557 1.614314 0.4002807 0.2343185 -1.6170558 0.10459167 0.1383667 0.07426667 -0.06410000 4.829124e-01 8.874548e-16 FALSE TRUE
MSTRG.8074.4 ENSG00000122965 HEK293_OSMI2_6hA HEK293_TMG_6hB RBM19 protein_coding   26.60934 38.95185 21.60329 5.802447 0.8196147 -0.8501432 0.9349666 0.000000 2.887553 0.0000000 0.7162678 8.1786913 0.04780833 0.0000000 0.13276667 0.13276667 8.874548e-16 8.874548e-16 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000122965 E001 0.695847 0.1461372199 1.755844e-01   12 113816738 113816741 4 - 0.001 0.310 9.416
ENSG00000122965 E002 15.842804 0.0443804473 1.744291e-01 0.3964250636 12 113816742 113816843 102 - 1.047 1.246 0.710
ENSG00000122965 E003 33.748788 0.0367017811 6.666235e-02 0.2175570720 12 113816844 113817074 231 - 1.330 1.562 0.798
ENSG00000122965 E004 16.585154 0.0058919376 2.892358e-01 0.5297722959 12 113817075 113817121 47 - 1.119 1.238 0.423
ENSG00000122965 E005 37.483932 0.0281861062 3.161388e-02 0.1321021488 12 113817122 113817361 240 - 1.368 1.611 0.838
ENSG00000122965 E006 79.365019 0.0381146610 9.667785e-02 0.2759352593 12 113817362 113817925 564 - 1.722 1.918 0.661
ENSG00000122965 E007 29.246923 0.0548513722 2.006853e-02 0.0961219076 12 113817926 113818102 177 - 1.178 1.524 1.204
ENSG00000122965 E008 23.013036 0.0856177811 1.346930e-01 0.3392103905 12 113818103 113818186 84 - 1.147 1.410 0.923
ENSG00000122965 E009 6.682541 0.1138046619 1.382667e-01 0.3449163731 12 113822054 113822056 3 - 0.595 0.925 1.338
ENSG00000122965 E010 352.402500 0.0015209287 9.779692e-06 0.0002056484 12 113822057 113822646 590 - 2.405 2.549 0.481
ENSG00000122965 E011 32.114804 0.0111944583 2.654041e-01 0.5047738216 12 113822647 113822686 40 - 1.396 1.509 0.390
ENSG00000122965 E012 83.555122 0.0054624774 9.248102e-01 0.9647294235 12 113822687 113822807 121 - 1.871 1.897 0.089
ENSG00000122965 E013 164.874796 0.0036232113 4.358450e-01 0.6556283599 12 113822808 113823089 282 - 2.191 2.180 -0.036
ENSG00000122965 E014 86.949964 0.0008841430 1.071347e-02 0.0611684463 12 113823090 113823208 119 - 1.810 1.941 0.441
ENSG00000122965 E015 267.468102 0.0062287858 1.411387e-01 0.3492385741 12 113823209 113823321 113 - 2.326 2.417 0.302
ENSG00000122965 E016 289.357744 0.0008764493 3.271225e-03 0.0250182037 12 113844668 113844788 121 - 2.357 2.454 0.324
ENSG00000122965 E017 265.439940 0.0017677795 5.762900e-03 0.0385562359 12 113858791 113858896 106 - 2.311 2.415 0.347
ENSG00000122965 E018 0.333790 0.0341275269 5.351969e-01   12 113858897 113858904 8 - 0.172 0.084 -1.181
ENSG00000122965 E019 314.540482 0.0011881914 3.587066e-02 0.1438714967 12 113914969 113915085 117 - 2.406 2.480 0.248
ENSG00000122965 E020 227.670648 0.0003026157 8.956435e-01 0.9495384499 12 113918392 113918447 56 - 2.308 2.328 0.069
ENSG00000122965 E021 248.929608 0.0036095046 5.823491e-01 0.7586222515 12 113920611 113920690 80 - 2.332 2.374 0.141
ENSG00000122965 E022 216.108065 0.0020596641 7.910852e-01 0.8910941497 12 113924697 113924757 61 - 2.281 2.308 0.091
ENSG00000122965 E023 326.541488 0.0382827190 2.640436e-01 0.5033226140 12 113927054 113927229 176 - 2.402 2.507 0.349
ENSG00000122965 E024 1.751400 0.1653562388 5.170388e-01 0.7130276772 12 113927230 113927431 202 - 0.295 0.442 0.860
ENSG00000122965 E025 217.997744 1.2412436524 4.524514e-01 0.6674974390 12 113937007 113937136 130 - 2.147 2.356 0.698
ENSG00000122965 E026 256.744478 0.0034172250 6.453814e-03 0.0419460473 12 113939960 113940160 201 - 2.291 2.407 0.389
ENSG00000122965 E027 201.882626 0.0004267640 6.080212e-02 0.2046334276 12 113942324 113942434 111 - 2.295 2.260 -0.118
ENSG00000122965 E028 216.289621 0.0008294489 1.121584e-03 0.0108320478 12 113945828 113945924 97 - 2.352 2.277 -0.251
ENSG00000122965 E029 234.100530 0.0002834879 4.095094e-04 0.0048227547 12 113946354 113946475 122 - 2.385 2.313 -0.240
ENSG00000122965 E030 245.196443 0.0003284565 1.748701e-02 0.0872454183 12 113947334 113947464 131 - 2.385 2.342 -0.141
ENSG00000122965 E031 327.959818 0.0022589568 2.465815e-03 0.0201109778 12 113948833 113949036 204 - 2.529 2.455 -0.246
ENSG00000122965 E032 196.611759 0.0035579725 3.228793e-02 0.1340887595 12 113950083 113950154 72 - 2.302 2.235 -0.224
ENSG00000122965 E033 178.563589 0.0002938869 2.560471e-03 0.0207238047 12 113952512 113952590 79 - 2.263 2.193 -0.232
ENSG00000122965 E034 175.226604 0.0004547387 8.521244e-02 0.2550117890 12 113955131 113955211 81 - 2.234 2.201 -0.113
ENSG00000122965 E035 189.175045 0.0002811692 1.330070e-02 0.0716408512 12 113957782 113957862 81 - 2.278 2.226 -0.173
ENSG00000122965 E036 268.598502 0.0023627448 1.234894e-02 0.0678298990 12 113957863 113958050 188 - 2.433 2.372 -0.204
ENSG00000122965 E037 266.922445 0.0035618705 3.264851e-02 0.1350912590 12 113959212 113959404 193 - 2.429 2.369 -0.199
ENSG00000122965 E038 125.457328 0.0025660472 2.752267e-03 0.0218839918 12 113959865 113959903 39 - 2.134 2.027 -0.357
ENSG00000122965 E039 192.956631 0.0002906682 7.912702e-06 0.0001713978 12 113960059 113960178 120 - 2.321 2.216 -0.352
ENSG00000122965 E040 233.244135 0.0002467056 5.770091e-04 0.0063716373 12 113962232 113962414 183 - 2.381 2.312 -0.231
ENSG00000122965 E041 1.188360 0.1620784569 9.244374e-03   12 113966136 113966191 56 - 0.598 0.085 -3.782
ENSG00000122965 E042 141.633944 0.0033296727 1.344778e-01 0.3388390836 12 113966192 113966381 190 - 2.148 2.103 -0.149