ENSG00000122484

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000484158 ENSG00000122484 HEK293_OSMI2_6hA HEK293_TMG_6hB RPAP2 protein_coding processed_transcript 13.24971 4.059942 24.91771 0.6940848 0.8289049 2.61467 11.438336 3.0894569 22.220083 0.62561990 0.8084313 2.842426 0.8021792 0.7512333 0.89153333 0.1403000 0.005539711 0.005539711 FALSE FALSE
ENST00000610020 ENSG00000122484 HEK293_OSMI2_6hA HEK293_TMG_6hB RPAP2 protein_coding protein_coding 13.24971 4.059942 24.91771 0.6940848 0.8289049 2.61467 1.632607 0.8490022 2.352824 0.05619989 0.1374275 1.459778 0.1768708 0.2181333 0.09456667 -0.1235667 0.011067909 0.005539711 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000122484 E001 3.234983 0.080577332 9.922311e-01 9.978132e-01 1 92299037 92299058 22 + 0.541 0.634 0.419
ENSG00000122484 E002 11.672959 0.097819489 7.946687e-01 8.931286e-01 1 92299059 92299084 26 + 0.969 1.153 0.671
ENSG00000122484 E003 22.736382 0.076824175 4.893497e-01 6.935545e-01 1 92299085 92299146 62 + 1.272 1.315 0.152
ENSG00000122484 E004 31.705176 0.031887934 7.837489e-02 2.415406e-01 1 92300194 92300239 46 + 1.426 1.391 -0.121
ENSG00000122484 E005 54.826349 0.046594829 3.797786e-02 1.495574e-01 1 92301476 92301590 115 + 1.662 1.593 -0.234
ENSG00000122484 E006 63.264894 0.035415086 1.192589e-03 1.137548e-02 1 92303977 92304075 99 + 1.739 1.572 -0.569
ENSG00000122484 E007 64.694388 0.005057299 5.417130e-08 2.143914e-06 1 92304284 92304349 66 + 1.757 1.549 -0.707
ENSG00000122484 E008 77.909893 0.008812051 3.371429e-04 4.111347e-03 1 92307188 92307276 89 + 1.821 1.720 -0.339
ENSG00000122484 E009 56.057060 0.061499673 1.916986e-01 4.190241e-01 1 92320599 92320634 36 + 1.674 1.598 -0.260
ENSG00000122484 E010 302.436230 1.725388686 6.920318e-01 8.303539e-01 1 92323445 92324375 931 + 2.349 2.494 0.484
ENSG00000122484 E011 1.042501 0.016413008 3.091672e-03   1 92333242 92333390 149 + 0.063 0.635 4.401
ENSG00000122484 E012 65.077282 0.057331363 8.401469e-01 9.195014e-01 1 92333391 92333473 83 + 1.692 1.826 0.454
ENSG00000122484 E013 69.308915 0.011201975 9.674270e-01 9.856573e-01 1 92336347 92336427 81 + 1.717 1.863 0.492
ENSG00000122484 E014 57.779060 0.012227378 4.392712e-02 1.648947e-01 1 92345846 92345914 69 + 1.672 1.680 0.025
ENSG00000122484 E015 4.490512 0.106454364 4.306544e-01 6.516414e-01 1 92357557 92357632 76 + 0.609 0.844 0.964
ENSG00000122484 E016 4.820357 0.090395833 3.746282e-01 6.078313e-01 1 92357946 92358043 98 + 0.628 0.882 1.027
ENSG00000122484 E017 83.494183 0.033170632 1.365568e-01 3.422039e-01 1 92380724 92380873 150 + 1.822 1.851 0.098
ENSG00000122484 E018 498.691450 0.075970636 5.315997e-02 1.871587e-01 1 92387011 92402056 15046 + 2.508 2.846 1.124