ENSG00000122482

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000337393 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding protein_coding 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 6.2181004 0.93530896 12.0363208 0.46994110 0.8095703 3.6716628 0.28185000 0.18766667 0.421866667 0.23420000 0.65283976 0.01199558 FALSE TRUE
ENST00000347275 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding protein_coding 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 0.9012997 0.66449462 0.1194122 0.49134168 0.1194122 -2.3818330 0.16018750 0.13696667 0.003966667 -0.13300000 0.42511979 0.01199558 FALSE FALSE
ENST00000361321 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding protein_coding 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 2.5472405 0.57497887 5.8921934 0.22593705 0.4346917 3.3347948 0.13153750 0.14363333 0.204166667 0.06053333 0.78592759 0.01199558 FALSE FALSE
ENST00000370440 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding protein_coding 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 1.4491429 0.52083214 3.0968131 0.37529062 0.9779930 2.5491078 0.08566250 0.15513333 0.104266667 -0.05086667 0.94697604 0.01199558 FALSE TRUE
ENST00000482467 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding processed_transcript 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 0.5735321 0.69552977 0.3903297 0.11045390 0.3903297 -0.8175185 0.08617083 0.16293333 0.013000000 -0.14993333 0.07765350 0.01199558 FALSE FALSE
ENST00000495966 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding processed_transcript 16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 0.7749110 0.50436442 0.6149075 0.04462111 0.3219310 0.2808518 0.07630417 0.11860000 0.021500000 -0.09710000 0.34900705 0.01199558   FALSE
MSTRG.1585.7 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding   16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 1.5902567 0.08575375 2.2458365 0.04963420 0.3501945 4.5581898 0.06374167 0.01746667 0.077400000 0.05993333 0.05374095 0.01199558 FALSE TRUE
MSTRG.1585.8 ENSG00000122482 HEK293_OSMI2_6hA HEK293_TMG_6hB ZNF644 protein_coding   16.7409 4.349313 28.92906 0.6420708 1.600269 2.730846 0.7743642 0.01868169 1.9046301 0.01868169 0.6120007 6.0607921 0.02870000 0.00360000 0.064533333 0.06093333 0.01199558 0.01199558 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000122482 E001 0.1308682 0.030752285 5.410535e-02   1 90915298 90915303 6 - 0.000 0.245 18.925
ENSG00000122482 E002 108.6642442 1.323463016 2.692082e-01 5.089759e-01 1 90915304 90916184 881 - 1.836 2.149 1.050
ENSG00000122482 E003 81.5445117 1.229869661 3.103770e-01 5.508728e-01 1 90916185 90916389 205 - 1.734 1.965 0.776
ENSG00000122482 E004 61.4311532 0.893792369 2.933523e-01 5.340634e-01 1 90916390 90916489 100 - 1.628 1.795 0.566
ENSG00000122482 E005 38.6188197 0.751416096 2.585258e-01 4.973503e-01 1 90916490 90916495 6 - 1.429 1.612 0.628
ENSG00000122482 E006 118.1880659 1.387150018 4.545370e-01 6.691470e-01 1 90916496 90916790 295 - 1.922 2.018 0.325
ENSG00000122482 E007 108.4832335 1.359024823 4.882851e-01 6.928458e-01 1 90916791 90916990 200 - 1.892 1.951 0.200
ENSG00000122482 E008 93.6898537 0.038713527 5.660133e-01 7.473907e-01 1 90918052 90918154 103 - 1.846 1.809 -0.124
ENSG00000122482 E009 0.4654660 0.022019058 1.000000e+00   1 90918155 90918391 237 - 0.149 0.000 -15.607
ENSG00000122482 E010 4.3645332 0.005738570 6.857299e-01 8.263346e-01 1 90935408 90935528 121 - 0.646 0.516 -0.586
ENSG00000122482 E011 215.4094687 0.013069321 3.820853e-01 6.139387e-01 1 90937485 90938090 606 - 2.213 2.098 -0.385
ENSG00000122482 E012 752.2238408 0.071635089 1.191405e-03 1.136674e-02 1 90938272 90940948 2677 - 2.777 2.429 -1.158
ENSG00000122482 E013 120.7864351 0.017326265 6.854803e-05 1.089838e-03 1 90940949 90941309 361 - 1.990 1.605 -1.303
ENSG00000122482 E014 0.0000000       1 90972744 90973152 409 -      
ENSG00000122482 E015 0.3206185 0.025359925 1.000000e+00   1 90973153 90973213 61 - 0.105 0.000 -15.023
ENSG00000122482 E016 53.1748727 0.022630606 1.363594e-01 3.419415e-01 1 90982310 90982370 61 - 1.625 1.425 -0.683
ENSG00000122482 E017 1.8579653 0.168237961 1.033811e-01 2.878868e-01 1 91020448 91020528 81 - 0.296 0.609 1.653
ENSG00000122482 E018 5.5918743 0.008276529 1.832647e-05 3.547135e-04 1 91020529 91020636 108 - 0.556 1.094 2.135
ENSG00000122482 E019 39.5690653 0.001837729 1.312176e-07 4.745265e-06 1 91020637 91021146 510 - 1.413 1.703 0.989
ENSG00000122482 E020 15.7504170 0.002988980 3.708689e-08 1.527579e-06 1 91021147 91021201 55 - 0.983 1.441 1.628
ENSG00000122482 E021 5.1164362 0.005978961 4.740100e-01 6.828294e-01 1 91021273 91021473 201 - 0.708 0.516 -0.846
ENSG00000122482 E022 20.2654777 0.011653878 3.613179e-06 8.699856e-05 1 91021689 91021989 301 - 1.111 1.505 1.380
ENSG00000122482 E023 44.3362806 0.045784353 9.704801e-01 9.871005e-01 1 91021990 91022274 285 - 1.529 1.494 -0.120