ENSG00000122335

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000642244 ENSG00000122335 HEK293_OSMI2_6hA HEK293_TMG_6hB SERAC1 protein_coding protein_coding 5.963365 2.192553 8.159257 0.3694583 0.4777647 1.891028 0.58837773 0.0000000 1.3041748 0.00000000 0.29285759 7.038013 0.07468750 0.00000000 0.15996667 0.15996667 3.129650e-05 1.052371e-05 FALSE TRUE
ENST00000644972 ENSG00000122335 HEK293_OSMI2_6hA HEK293_TMG_6hB SERAC1 protein_coding protein_coding 5.963365 2.192553 8.159257 0.3694583 0.4777647 1.891028 0.35066842 0.0626405 0.5022570 0.06264050 0.09937162 2.818022 0.05505000 0.04213333 0.06186667 0.01973333 7.095960e-01 1.052371e-05 FALSE TRUE
ENST00000645077 ENSG00000122335 HEK293_OSMI2_6hA HEK293_TMG_6hB SERAC1 protein_coding nonsense_mediated_decay 5.963365 2.192553 8.159257 0.3694583 0.4777647 1.891028 0.09080297 0.2184593 0.1087733 0.08962857 0.10877334 -0.943726 0.02554167 0.09213333 0.01340000 -0.07873333 2.724570e-01 1.052371e-05 TRUE TRUE
ENST00000647468 ENSG00000122335 HEK293_OSMI2_6hA HEK293_TMG_6hB SERAC1 protein_coding protein_coding 5.963365 2.192553 8.159257 0.3694583 0.4777647 1.891028 2.90239591 1.5925143 3.2253919 0.30576211 0.32208080 1.013603 0.55868750 0.71900000 0.40263333 -0.31636667 2.122708e-02 1.052371e-05 FALSE TRUE
MSTRG.29242.7 ENSG00000122335 HEK293_OSMI2_6hA HEK293_TMG_6hB SERAC1 protein_coding   5.963365 2.192553 8.159257 0.3694583 0.4777647 1.891028 0.60806565 0.0000000 1.7447465 0.00000000 0.52891974 7.455119 0.06659583 0.00000000 0.20846667 0.20846667 1.052371e-05 1.052371e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000122335 E001 1.9235569 0.0109063799 2.604336e-01 0.4993990958 6 158109519 158109534 16 - 0.372 0.567 0.987
ENSG00000122335 E002 2.3042750 0.0095292905 1.912772e-01 0.4184693421 6 158109535 158109540 6 - 0.406 0.626 1.058
ENSG00000122335 E003 15.1084697 0.0027881551 1.025444e-04 0.0015275791 6 158109541 158109690 150 - 1.029 1.373 1.223
ENSG00000122335 E004 9.8210050 0.0037331856 5.325049e-03 0.0363208241 6 158109691 158109692 2 - 0.880 1.176 1.088
ENSG00000122335 E005 46.8185914 0.0046796327 5.604527e-06 0.0001273111 6 158109693 158110224 532 - 1.531 1.800 0.915
ENSG00000122335 E006 31.2956850 0.0013509888 4.036290e-01 0.6312266530 6 158110225 158110598 374 - 1.440 1.493 0.183
ENSG00000122335 E007 8.5550698 0.0036695188 3.899378e-02 0.1521713562 6 158110599 158110619 21 - 0.857 1.090 0.866
ENSG00000122335 E008 6.2941386 0.0048777779 2.283327e-02 0.1054290587 6 158110620 158110626 7 - 0.721 1.006 1.102
ENSG00000122335 E009 15.3415340 0.0131966081 3.668468e-01 0.6013662341 6 158110627 158110667 41 - 1.140 1.232 0.325
ENSG00000122335 E010 34.0472794 0.0016958600 1.563499e-01 0.3713982099 6 158110668 158110871 204 - 1.466 1.556 0.306
ENSG00000122335 E011 99.1872079 0.0006444335 5.657806e-01 0.7472282068 6 158110872 158111502 631 - 1.942 1.961 0.062
ENSG00000122335 E012 25.1129544 0.0019485161 2.624216e-03 0.0211151159 6 158111503 158113448 1946 - 1.301 1.514 0.740
ENSG00000122335 E013 45.6333627 0.0094270594 9.361389e-01 0.9703005055 6 158113449 158113592 144 - 1.617 1.607 -0.035
ENSG00000122335 E014 0.0000000       6 158114471 158114534 64 -      
ENSG00000122335 E015 0.0000000       6 158114535 158114592 58 -      
ENSG00000122335 E016 1.1008357 0.3843434948 7.879503e-01   6 158114593 158114730 138 - 0.255 0.328 0.498
ENSG00000122335 E017 2.4034957 0.0953724388 8.512225e-01 0.9257049213 6 158114731 158114788 58 - 0.472 0.501 0.143
ENSG00000122335 E018 28.8695479 0.0014679074 9.748927e-01 0.9893431122 6 158114789 158114825 37 - 1.421 1.420 -0.005
ENSG00000122335 E019 48.7248650 0.0010864234 3.346200e-01 0.5732134058 6 158114826 158114971 146 - 1.658 1.599 -0.201
ENSG00000122335 E020 35.3129366 0.0064980750 3.744171e-01 0.6076919498 6 158116185 158116282 98 - 1.525 1.455 -0.241
ENSG00000122335 E021 4.2923366 0.0210325070 6.579901e-01 0.8086512736 6 158116283 158117531 1249 - 0.705 0.626 -0.334
ENSG00000122335 E022 0.5008152 0.0416668257 4.040485e-01   6 158117532 158117575 44 - 0.199 0.000 -12.621
ENSG00000122335 E023 0.6268817 0.1619675452 3.065320e-01   6 158117576 158117726 151 - 0.249 0.000 -12.706
ENSG00000122335 E024 27.9227268 0.0013535994 4.363163e-02 0.1642101286 6 158117727 158117821 95 - 1.446 1.285 -0.557
ENSG00000122335 E025 30.1473927 0.0172720414 2.008356e-02 0.0961770338 6 158119029 158119120 92 - 1.500 1.260 -0.834
ENSG00000122335 E026 26.1360348 0.0273181665 1.098829e-01 0.2988236175 6 158119121 158119170 50 - 1.430 1.236 -0.676
ENSG00000122335 E027 38.0249163 0.0092254963 3.537746e-01 0.5906171478 6 158120425 158120575 151 - 1.564 1.485 -0.268
ENSG00000122335 E028 36.0226626 0.0154367803 8.635537e-01 0.9323592899 6 158128108 158128270 163 - 1.530 1.510 -0.066
ENSG00000122335 E029 30.0825531 0.0212095122 7.840093e-01 0.8868974329 6 158130373 158130486 114 - 1.457 1.424 -0.112
ENSG00000122335 E030 34.3663522 0.0070922340 9.444542e-01 0.9745734592 6 158143056 158143184 129 - 1.503 1.506 0.010
ENSG00000122335 E031 30.2868153 0.0023409897 1.447632e-02 0.0761566135 6 158144299 158144420 122 - 1.489 1.297 -0.664
ENSG00000122335 E032 0.1723744 0.0398687916 1.655528e-01   6 158145264 158145363 100 - 0.000 0.187 12.497
ENSG00000122335 E033 2.9301748 0.1162114844 6.352706e-02 0.2106483497 6 158145920 158146781 862 - 0.437 0.797 1.598
ENSG00000122335 E034 28.8914575 0.0032301453 1.866997e-02 0.0912955130 6 158146782 158146869 88 - 1.466 1.273 -0.671
ENSG00000122335 E035 17.3364517 0.0023530592 5.552507e-02 0.1925345824 6 158146870 158146913 44 - 1.261 1.070 -0.680
ENSG00000122335 E036 20.5217785 0.0015804412 1.775833e-01 0.4005542045 6 158148865 158148954 90 - 1.313 1.191 -0.429
ENSG00000122335 E037 25.2888811 0.0016604188 8.038428e-02 0.2456117179 6 158150453 158150563 111 - 1.405 1.260 -0.504
ENSG00000122335 E038 11.5413906 0.0176378354 3.705228e-01 0.6043776062 6 158150564 158150587 24 - 1.075 0.958 -0.428
ENSG00000122335 E039 9.0177977 0.0123039697 4.327045e-01 0.6532824969 6 158150588 158150589 2 - 0.979 0.873 -0.399
ENSG00000122335 E040 0.4576437 0.2000658109 7.415743e-01   6 158151039 158151129 91 - 0.141 0.188 0.500
ENSG00000122335 E041 0.0000000       6 158152756 158152842 87 -      
ENSG00000122335 E042 8.8366210 0.0055736603 2.884395e-01 0.5291570720 6 158155315 158155351 37 - 0.979 0.840 -0.527
ENSG00000122335 E043 21.4251649 0.0155390711 3.587646e-01 0.5947984237 6 158158273 158158364 92 - 1.329 1.232 -0.341
ENSG00000122335 E044 0.9452873 0.0162943706 1.155458e-01   6 158158365 158159298 934 - 0.335 0.000 -13.577
ENSG00000122335 E045 3.3636020 0.0069787890 4.003606e-02 0.1548228012 6 158160932 158162147 1216 - 0.495 0.805 1.337
ENSG00000122335 E046 0.1308682 0.0307874354 1.661536e-01   6 158162148 158162152 5 - 0.000 0.187 12.556
ENSG00000122335 E047 0.1779838 0.0346380399 1.000000e+00   6 158166114 158166159 46 - 0.077 0.000 -11.138
ENSG00000122335 E048 17.7202018 0.0078119982 7.297745e-01 0.8541001542 6 158168140 158168280 141 - 1.217 1.247 0.103