ENSG00000121644

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302550 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding protein_coding 36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 17.3097198 5.1039209 28.7223151 2.5225084 1.94358155 2.4901725 0.40857083 0.28920000 0.493933333 0.20473333 6.446435e-01 4.748325e-08 FALSE TRUE
ENST00000484738 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding processed_transcript 36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 0.9974347 1.4347149 1.1847599 0.3469669 0.09962788 -0.2740641 0.03938750 0.10213333 0.020400000 -0.08173333 4.748325e-08 4.748325e-08 TRUE FALSE
MSTRG.3389.15 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding   36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 2.8793062 2.3503664 7.4593228 0.8792084 2.18356793 1.6619666 0.09471250 0.22166667 0.130733333 -0.09093333 9.551317e-01 4.748325e-08 FALSE TRUE
MSTRG.3389.17 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding   36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 5.1169986 0.8453153 11.7626313 0.8453153 1.39551945 3.7828366 0.17402917 0.04823333 0.202166667 0.15393333 2.254452e-01 4.748325e-08   FALSE
MSTRG.3389.2 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding   36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 1.1741049 1.3309221 0.3148802 0.8456814 0.18910886 -2.0452456 0.05440833 0.07256667 0.005166667 -0.06740000 6.317764e-01 4.748325e-08 TRUE FALSE
MSTRG.3389.4 ENSG00000121644 HEK293_OSMI2_6hA HEK293_TMG_6hB DESI2 protein_coding   36.79126 14.5512 58.05488 3.55887 2.669927 1.995537 4.9913739 2.0521924 3.3952560 0.9105751 0.72565437 0.7235843 0.11585417 0.11956667 0.057866667 -0.06170000 7.346726e-01 4.748325e-08 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000121644 E001 1.5475207 0.0143262151 9.352717e-01 9.698101e-01 1 244653057 244653065 9 + 0.341 0.347 0.036
ENSG00000121644 E002 1.5004050 0.0124100776 4.031836e-01 6.308650e-01 1 244653066 244653068 3 + 0.306 0.452 0.843
ENSG00000121644 E003 1.7621413 0.0109156783 7.791062e-02 2.406262e-01 1 244653069 244653074 6 + 0.306 0.608 1.577
ENSG00000121644 E004 1.4227417 0.0127494178 2.705454e-02 1.184756e-01 1 244653075 244653079 5 + 0.226 0.608 2.163
ENSG00000121644 E005 1.2447579 0.0136430321 1.307242e-02   1 244653080 244653081 2 + 0.179 0.608 2.579
ENSG00000121644 E006 4.1258981 0.0255213189 3.264649e-05 5.798816e-04 1 244653082 244653102 21 + 0.432 1.052 2.592
ENSG00000121644 E007 8.4799046 0.0278981928 2.236566e-07 7.605532e-06 1 244653103 244653125 23 + 0.662 1.319 2.466
ENSG00000121644 E008 11.4136974 0.0540421062 1.235253e-06 3.412074e-05 1 244653126 244653140 15 + 0.764 1.442 2.473
ENSG00000121644 E009 106.6652823 0.0088832433 1.974029e-05 3.781562e-04 1 244653141 244653355 215 + 1.889 2.130 0.810
ENSG00000121644 E010 21.7901948 0.0054419303 1.523076e-17 3.261706e-15 1 244653356 244653750 395 + 1.036 1.700 2.316
ENSG00000121644 E011 9.5726358 0.0216915523 3.363707e-06 8.193269e-05 1 244653751 244653794 44 + 0.761 1.321 2.065
ENSG00000121644 E012 12.6445330 0.0392058014 1.868150e-04 2.527323e-03 1 244653795 244653912 118 + 0.912 1.377 1.673
ENSG00000121644 E013 10.6377258 0.0173506308 6.303004e-10 3.810591e-08 1 244653913 244653938 26 + 0.734 1.430 2.550
ENSG00000121644 E014 20.1519020 0.0066611859 4.414413e-16 7.744514e-14 1 244653939 244654025 87 + 1.002 1.667 2.329
ENSG00000121644 E015 4.6490762 0.0352341486 5.310799e-02 1.870235e-01 1 244654026 244654037 12 + 0.593 0.888 1.202
ENSG00000121644 E016 15.9988395 0.0544354529 3.113586e-11 2.453636e-09 1 244656296 244656549 254 + 0.734 1.669 3.370
ENSG00000121644 E017 0.4466850 0.0244048088 5.914298e-01   1 244672404 244672418 15 + 0.179 0.000 -31.563
ENSG00000121644 E018 0.5893196 0.1109510870 4.364296e-01   1 244672419 244672461 43 + 0.224 0.000 -32.062
ENSG00000121644 E019 3.1411297 0.0081363970 4.907496e-01 6.945048e-01 1 244681868 244681932 65 + 0.591 0.452 -0.661
ENSG00000121644 E020 121.3776579 0.0003903167 7.097396e-03 4.506304e-02 1 244686597 244686669 73 + 2.036 1.908 -0.428
ENSG00000121644 E021 120.3477017 0.0003382363 2.001852e-03 1.708087e-02 1 244689249 244689342 94 + 2.034 1.888 -0.488
ENSG00000121644 E022 86.3336227 0.0006128548 2.827106e-04 3.554247e-03 1 244691879 244691880 2 + 1.899 1.698 -0.679
ENSG00000121644 E023 86.4728271 0.0005194176 6.066239e-05 9.822529e-04 1 244691881 244691883 3 + 1.903 1.681 -0.747
ENSG00000121644 E024 203.8698370 0.0002558987 2.175219e-05 4.102646e-04 1 244691884 244692020 137 + 2.262 2.109 -0.512
ENSG00000121644 E025 5.1520911 0.0058241861 2.145442e-01 4.469811e-01 1 244699861 244700033 173 + 0.761 0.537 -0.964
ENSG00000121644 E026 2859.9687459 0.0057860071 2.189236e-02 1.022251e-01 1 244705556 244709033 3478 + 3.386 3.340 -0.153