ENSG00000121361

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000240662 ENSG00000121361 HEK293_OSMI2_6hA HEK293_TMG_6hB KCNJ8 protein_coding protein_coding 4.798947 2.637534 6.396989 0.4687689 0.4268232 1.274997 3.4413594 1.840379 3.181506 0.4563233 0.2044848 0.7864163 0.73769167 0.7688667 0.4991333 -0.2697333 3.598826e-01 8.755342e-19 FALSE TRUE
ENST00000657855 ENSG00000121361 HEK293_OSMI2_6hA HEK293_TMG_6hB KCNJ8 protein_coding processed_transcript 4.798947 2.637534 6.396989 0.4687689 0.4268232 1.274997 0.5647973 0.000000 3.215483 0.0000000 0.3341110 8.3333715 0.09268333 0.0000000 0.5008667 0.5008667 8.755342e-19 8.755342e-19 FALSE TRUE
ENST00000667884 ENSG00000121361 HEK293_OSMI2_6hA HEK293_TMG_6hB KCNJ8 protein_coding protein_coding 4.798947 2.637534 6.396989 0.4687689 0.4268232 1.274997 0.7218455 0.797155 0.000000 0.7971550 0.0000000 -6.3347738 0.15242083 0.2311333 0.0000000 -0.2311333 8.210107e-01 8.755342e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000121361 E001 0.0000000       12 21764955 21764983 29 -      
ENSG00000121361 E002 0.1723744 0.042384409 0.295575857   12 21764984 21764987 4 - 0.000 0.152 10.492
ENSG00000121361 E003 0.3150090 0.028632378 0.696386073   12 21764988 21764989 2 - 0.096 0.152 0.765
ENSG00000121361 E004 177.0383054 0.001353130 0.001094719 0.01063488 12 21764990 21766623 1634 - 2.259 2.202 -0.192
ENSG00000121361 E005 19.3771331 0.049219505 0.246871445 0.48454688 12 21773243 21773274 32 - 1.251 1.368 0.412
ENSG00000121361 E006 28.4975456 0.006645765 0.016747433 0.08454014 12 21773275 21773368 94 - 1.387 1.556 0.579
ENSG00000121361 E007 43.4143798 0.021949646 0.712745219 0.84345807 12 21773369 21773686 318 - 1.613 1.660 0.159
ENSG00000121361 E008 0.1779838 0.056381649 0.773513537   12 21773687 21773762 76 - 0.096 0.000 -11.628
ENSG00000121361 E009 14.7062644 0.002567575 0.128758026 0.32983199 12 21774546 21774683 138 - 1.126 1.261 0.477
ENSG00000121361 E010 6.3256503 0.005644054 0.473373149 0.68236693 12 21774684 21774770 87 - 0.899 0.810 -0.345
ENSG00000121361 E011 0.0000000       12 21774897 21775009 113 -      
ENSG00000121361 E012 0.0000000       12 21775010 21775056 47 -      
ENSG00000121361 E013 0.0000000       12 21775487 21775600 114 -