ENSG00000121068

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000240328 ENSG00000121068 HEK293_OSMI2_6hA HEK293_TMG_6hB TBX2 protein_coding protein_coding 10.58843 18.16783 5.711778 3.624512 0.2177616 -1.667644 5.697792 9.902944 1.785648 2.2586630 0.0907728 -2.4648092 0.5075167 0.5382333 0.3123667 -0.2258667 0.0001832438 0.0001832438 FALSE TRUE
ENST00000477081 ENSG00000121068 HEK293_OSMI2_6hA HEK293_TMG_6hB TBX2 protein_coding retained_intron 10.58843 18.16783 5.711778 3.624512 0.2177616 -1.667644 1.678429 2.600159 1.311651 0.3200931 0.1540777 -0.9817959 0.1768500 0.1662333 0.2317333 0.0655000 0.7522618559 0.0001832438 FALSE TRUE
MSTRG.14844.3 ENSG00000121068 HEK293_OSMI2_6hA HEK293_TMG_6hB TBX2 protein_coding   10.58843 18.16783 5.711778 3.624512 0.2177616 -1.667644 3.158472 5.664722 2.508135 1.6971715 0.2277662 -1.1721940 0.3105583 0.2955333 0.4373667 0.1418333 0.4223686941 0.0001832438 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000121068 E001 6.378638 0.0043925998 4.362863e-01 6.560038e-01 17 61399843 61399909 67 + 0.694 0.805 0.449
ENSG00000121068 E002 13.919314 0.0049923359 3.873659e-02 1.514565e-01 17 61399910 61400364 455 + 0.879 1.129 0.924
ENSG00000121068 E003 58.104048 0.0037651406 3.705888e-01 6.044014e-01 17 61400365 61400571 207 + 1.723 1.654 -0.234
ENSG00000121068 E004 46.274405 0.0064748866 5.158459e-01 7.121640e-01 17 61401684 61401705 22 + 1.620 1.559 -0.207
ENSG00000121068 E005 175.529738 0.0033479389 1.696693e-02 8.533360e-02 17 61401706 61401951 246 + 2.228 2.122 -0.356
ENSG00000121068 E006 159.154137 0.0005274265 6.872029e-02 2.221854e-01 17 61403061 61403207 147 + 2.163 2.091 -0.240
ENSG00000121068 E007 112.891667 0.0014211842 5.474198e-02 1.907606e-01 17 61404421 61404497 77 + 2.031 1.940 -0.305
ENSG00000121068 E008 100.726383 0.0028036716 6.653498e-02 2.172765e-01 17 61404606 61404769 164 + 1.841 1.923 0.275
ENSG00000121068 E009 5.768272 0.1158209181 3.759048e-01 6.089428e-01 17 61404770 61405201 432 + 0.881 0.692 -0.754
ENSG00000121068 E010 168.022341 0.0023458875 5.760015e-09 2.848524e-07 17 61405202 61405836 635 + 1.927 2.166 0.802
ENSG00000121068 E011 130.793133 0.0653705715 1.181159e-01 3.127682e-01 17 61405837 61408053 2217 + 2.169 1.970 -0.666
ENSG00000121068 E012 266.250364 0.0025318340 2.317480e-03 1.916633e-02 17 61408054 61408721 668 + 2.253 2.343 0.302
ENSG00000121068 E013 117.758963 0.0006630681 7.931083e-01 8.922579e-01 17 61408722 61408841 120 + 1.977 1.975 -0.006
ENSG00000121068 E014 156.672946 0.0004584201 4.101722e-01 6.361134e-01 17 61408842 61409257 416 + 2.132 2.092 -0.134
ENSG00000121068 E015 21.231728 0.0339446750 7.658495e-01 8.761521e-01 17 61409258 61409466 209 + 1.292 1.235 -0.201