ENSG00000117748

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373912 ENSG00000117748 HEK293_OSMI2_6hA HEK293_TMG_6hB RPA2 protein_coding protein_coding 156.2899 98.48953 147.6266 20.66869 7.28671 0.5838617 145.0564 94.97652 132.0892 20.43319 7.6697 0.4758274 0.926925 0.9611333 0.8941 -0.06703333 0.03278321 0.03278321 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000117748 E001 1.24321 0.1887303109 2.271102e-01   1 27891524 27891537 14 - 0.200 0.453 1.646
ENSG00000117748 E002 484.78478 0.0002227018 1.120936e-05 2.314415e-04 1 27891538 27891708 171 - 2.647 2.722 0.250
ENSG00000117748 E003 1025.45614 0.0001956623 2.734518e-08 1.163578e-06 1 27891709 27891923 215 - 2.975 3.043 0.227
ENSG00000117748 E004 976.81353 0.0003648948 8.367498e-03 5.101395e-02 1 27891924 27892072 149 - 2.970 3.006 0.122
ENSG00000117748 E005 824.91187 0.0004968228 2.937842e-01 5.344200e-01 1 27892073 27892148 76 - 2.905 2.923 0.059
ENSG00000117748 E006 1115.18661 0.0001718162 2.120956e-01 4.442203e-01 1 27892149 27892247 99 - 3.037 3.051 0.048
ENSG00000117748 E007 1050.44214 0.0001605241 3.299700e-02 1.360604e-01 1 27894012 27894106 95 - 3.029 3.004 -0.085
ENSG00000117748 E008 1113.58194 0.0009735266 1.311059e-01 3.335696e-01 1 27894290 27894390 101 - 3.055 3.029 -0.087
ENSG00000117748 E009 624.91784 0.0001604791 1.698195e-05 3.324411e-04 1 27894391 27894397 7 - 2.821 2.756 -0.217
ENSG00000117748 E010 949.13971 0.0001122837 2.190399e-05 4.126236e-04 1 27897005 27897121 117 - 2.998 2.946 -0.172
ENSG00000117748 E011 665.18292 0.0001316987 2.793522e-01 5.194635e-01 1 27897633 27897707 75 - 2.829 2.813 -0.054
ENSG00000117748 E012 754.21142 0.0012224754 9.583731e-01 9.810592e-01 1 27906928 27907041 114 - 2.877 2.876 -0.004
ENSG00000117748 E013 648.77263 0.0012130375 9.788848e-01 9.912297e-01 1 27907181 27907280 100 - 2.811 2.811 -0.001
ENSG00000117748 E014 347.35709 0.0002021085 5.961227e-01 7.677889e-01 1 27907281 27907282 2 - 2.534 2.544 0.032
ENSG00000117748 E015 3.57419 0.0961393986 9.151202e-02 2.664916e-01 1 27913912 27914062 151 - 0.783 0.440 -1.532
ENSG00000117748 E016 586.01719 0.0001362518 8.158954e-01 9.058060e-01 1 27914063 27914169 107 - 2.768 2.764 -0.014
ENSG00000117748 E017 12.26146 0.1810738058 1.152384e-01 3.078564e-01 1 27914170 27914421 252 - 1.246 0.897 -1.270
ENSG00000117748 E018 10.13770 0.0598165194 3.835884e-01 6.152438e-01 1 27914422 27914433 12 - 1.094 0.956 -0.506
ENSG00000117748 E019 486.22689 0.0001398431 6.539359e-01 8.060487e-01 1 27914434 27914626 193 - 2.689 2.681 -0.027
ENSG00000117748 E020 26.81381 0.0012646289 1.587072e-05 3.133969e-04 1 27914627 27914746 120 - 1.555 1.248 -1.063