ENSG00000117650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366999 ENSG00000117650 HEK293_OSMI2_6hA HEK293_TMG_6hB NEK2 protein_coding protein_coding 24.62967 8.663453 43.99205 2.367744 1.816902 2.342892 14.973116 7.0614443 27.229425 2.0301088 1.149437 1.945619 0.6876458 0.80343333 0.6193000 -0.18413333 0.01305036 0.01305036 FALSE TRUE
ENST00000540251 ENSG00000117650 HEK293_OSMI2_6hA HEK293_TMG_6hB NEK2 protein_coding protein_coding 24.62967 8.663453 43.99205 2.367744 1.816902 2.342892 4.016897 0.4712584 6.696399 0.2961383 1.335621 3.800655 0.1120917 0.06236667 0.1531667 0.09080000 0.66289153 0.01305036 FALSE TRUE
MSTRG.3001.3 ENSG00000117650 HEK293_OSMI2_6hA HEK293_TMG_6hB NEK2 protein_coding   24.62967 8.663453 43.99205 2.367744 1.816902 2.342892 4.433853 0.7066971 7.901363 0.1800873 1.221478 3.464491 0.1466625 0.08426667 0.1785333 0.09426667 0.09632426 0.01305036 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000117650 E001 3.314959 0.0135573439 0.0317256880 0.132406466 1 211658657 211658712 56 - 0.477 0.820 1.489
ENSG00000117650 E002 145.158946 0.0003546361 0.0016128175 0.014417724 1 211662772 211663141 370 - 2.056 2.163 0.356
ENSG00000117650 E003 206.599769 0.0024902715 0.0931707095 0.269640924 1 211663142 211663402 261 - 2.221 2.279 0.194
ENSG00000117650 E004 147.758897 0.0004299748 0.0379273886 0.149421030 1 211663403 211663524 122 - 2.073 2.144 0.238
ENSG00000117650 E005 155.915829 0.0002866480 0.8439770832 0.921579234 1 211663525 211663628 104 - 2.113 2.107 -0.019
ENSG00000117650 E006 82.210321 0.0007482792 0.4406118582 0.659152967 1 211663629 211663652 24 - 1.825 1.863 0.127
ENSG00000117650 E007 19.199891 0.0047046070 0.9769396005 0.990234231 1 211666430 211667105 676 - 1.222 1.221 -0.003
ENSG00000117650 E008 192.119123 0.0002767393 0.5765042250 0.754780731 1 211667106 211667231 126 - 2.197 2.216 0.061
ENSG00000117650 E009 249.271396 0.0002543159 0.1190241428 0.314276961 1 211669113 211669332 220 - 2.304 2.346 0.139
ENSG00000117650 E010 1.130833 0.1070093878 0.6069315849   1 211669333 211669519 187 - 0.262 0.354 0.602
ENSG00000117650 E011 193.775184 0.0007338506 0.3638704963 0.598920080 1 211670281 211670393 113 - 2.197 2.225 0.095
ENSG00000117650 E012 96.043778 0.0052045744 0.5520630054 0.737754456 1 211670394 211670407 14 - 1.908 1.870 -0.128
ENSG00000117650 E013 2.815139 0.0092794538 0.2373540300 0.473493581 1 211671169 211671201 33 - 0.477 0.677 0.909
ENSG00000117650 E014 155.980963 0.0003332257 0.1232387981 0.320895161 1 211671202 211671284 83 - 2.120 2.067 -0.177
ENSG00000117650 E015 288.096177 0.0006215708 0.0009862557 0.009809588 1 211673483 211673723 241 - 2.395 2.307 -0.295
ENSG00000117650 E016 246.060566 0.0002797488 0.0002345022 0.003051170 1 211674296 211674513 218 - 2.328 2.225 -0.343
ENSG00000117650 E017 138.325509 0.0004370552 0.2936433450 0.534308267 1 211675384 211675647 264 - 2.067 2.028 -0.128