ENSG00000117505

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370267 ENSG00000117505 HEK293_OSMI2_6hA HEK293_TMG_6hB DR1 protein_coding protein_coding 21.28035 7.338467 36.15222 1.592417 0.7525248 2.298968 19.054031 6.032932 33.172644 1.2641543 0.74027247 2.457108 0.8800333 0.8268667 0.91753333 0.09066667 0.004750899 0.004750899 FALSE  
ENST00000370272 ENSG00000117505 HEK293_OSMI2_6hA HEK293_TMG_6hB DR1 protein_coding protein_coding 21.28035 7.338467 36.15222 1.592417 0.7525248 2.298968 2.211841 1.286911 2.948974 0.3402558 0.01125099 1.190017 0.1193583 0.1711333 0.08163333 -0.08950000 0.007112432 0.004750899 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000117505 E001 18.8286182 0.6750578812 4.213765e-01 6.446449e-01 1 93345907 93345934 28 + 1.194 1.355 0.564
ENSG00000117505 E002 101.2786666 0.0973713936 2.022797e-02 9.667288e-02 1 93345935 93346188 254 + 1.858 2.145 0.961
ENSG00000117505 E003 80.2673205 0.0043400660 6.313188e-05 1.017099e-03 1 93346189 93346312 124 + 1.804 1.978 0.582
ENSG00000117505 E004 569.0389051 0.0004127088 6.302335e-01 7.905582e-01 1 93346313 93346865 553 + 2.702 2.672 -0.097
ENSG00000117505 E005 363.0819800 0.0003025143 3.826918e-01 6.144568e-01 1 93353908 93354071 164 + 2.513 2.457 -0.187
ENSG00000117505 E006 0.8875026 0.0224431895 4.050380e-01   1 93354975 93355775 801 + 0.191 0.329 1.032
ENSG00000117505 E007 1154.3665119 0.0035874816 6.707350e-11 4.918231e-09 1 93360493 93362958 2466 + 3.038 2.867 -0.569
ENSG00000117505 E008 207.3893921 0.0003946721 4.640794e-25 2.381008e-22 1 93362959 93367442 4484 + 2.187 2.423 0.787
ENSG00000117505 E009 35.4984217 0.0140064420 2.285491e-04 2.985965e-03 1 93367443 93369493 2051 + 1.416 1.691 0.942