ENSG00000117500

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370280 ENSG00000117500 HEK293_OSMI2_6hA HEK293_TMG_6hB TMED5 protein_coding protein_coding 49.50674 13.48766 85.68074 2.240187 1.357149 2.66643 2.275019 1.015430 2.829943 0.5154040 0.0896837 1.469633 0.06491667 0.07676667 0.03306667 -0.0437000 0.695470262 0.001026108 FALSE TRUE
ENST00000370282 ENSG00000117500 HEK293_OSMI2_6hA HEK293_TMG_6hB TMED5 protein_coding protein_coding 49.50674 13.48766 85.68074 2.240187 1.357149 2.66643 5.896412 1.506613 9.491293 0.3433363 0.5793373 2.647271 0.12228750 0.10893333 0.11083333 0.0019000 1.000000000 0.001026108 FALSE TRUE
ENST00000370290 ENSG00000117500 HEK293_OSMI2_6hA HEK293_TMG_6hB TMED5 protein_coding nonsense_mediated_decay 49.50674 13.48766 85.68074 2.240187 1.357149 2.66643 24.457160 4.558852 46.500870 0.9499315 1.5418671 3.347664 0.45222500 0.33226667 0.54310000 0.2108333 0.001026108 0.001026108 FALSE TRUE
ENST00000479918 ENSG00000117500 HEK293_OSMI2_6hA HEK293_TMG_6hB TMED5 protein_coding protein_coding 49.50674 13.48766 85.68074 2.240187 1.357149 2.66643 15.096088 6.013829 26.764138 1.3566825 1.8237822 2.152087 0.32026250 0.44110000 0.31196667 -0.1291333 0.237674968 0.001026108 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000117500 E001 4.438042 0.012129344 1.014820e-02 5.876289e-02 1 93149742 93149788 47 - 0.544 0.929 1.582
ENSG00000117500 E002 14.808614 0.009284168 3.037321e-04 3.766438e-03 1 93149789 93149957 169 - 0.989 1.356 1.310
ENSG00000117500 E003 196.779680 0.004034165 1.691709e-01 3.894681e-01 1 93149958 93151857 1900 - 2.155 2.193 0.126
ENSG00000117500 E004 1784.571652 0.014727528 8.685258e-05 1.328191e-03 1 93151858 93154195 2338 - 3.140 2.959 -0.604
ENSG00000117500 E005 124.997576 0.031059727 9.877158e-04 9.819655e-03 1 93154196 93154231 36 - 2.006 1.657 -1.177
ENSG00000117500 E006 956.812263 0.002908427 2.742068e-01 5.141807e-01 1 93154232 93154888 657 - 2.854 2.803 -0.171
ENSG00000117500 E007 6.172741 0.094858995 3.383028e-01 5.764996e-01 1 93156036 93156039 4 - 0.706 0.885 0.707
ENSG00000117500 E008 7.873047 0.079081897 6.387965e-02 2.114336e-01 1 93156040 93156084 45 - 0.762 1.060 1.132
ENSG00000117500 E009 6.304005 0.062067461 1.584064e-02 8.121947e-02 1 93156085 93156299 215 - 0.649 1.041 1.532
ENSG00000117500 E010 532.862889 0.002423022 5.021667e-02 1.802148e-01 1 93156300 93156483 184 - 2.589 2.620 0.103
ENSG00000117500 E011 11.986443 0.036127945 1.026998e-07 3.798909e-06 1 93158815 93158885 71 - 0.786 1.454 2.426
ENSG00000117500 E012 296.520243 0.046460338 3.583890e-02 1.438065e-01 1 93160129 93160226 98 - 2.310 2.467 0.525
ENSG00000117500 E013 2.824693 0.008115199 1.710994e-01 3.919741e-01 1 93160227 93163210 2984 - 0.447 0.677 1.058
ENSG00000117500 E014 355.361071 1.835006723 3.936935e-01 6.232935e-01 1 93180054 93180516 463 - 2.350 2.659 1.028