ENSG00000117118

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375499 ENSG00000117118 HEK293_OSMI2_6hA HEK293_TMG_6hB SDHB protein_coding protein_coding 116.6305 52.71417 167.9167 6.101182 8.064463 1.671295 103.435282 50.91011 129.35127 6.852882 5.194419 1.345098 0.90253750 0.9609333 0.7714333 -0.1895000 9.235539e-03 1.268288e-40 FALSE TRUE
ENST00000491274 ENSG00000117118 HEK293_OSMI2_6hA HEK293_TMG_6hB SDHB protein_coding protein_coding 116.6305 52.71417 167.9167 6.101182 8.064463 1.671295 9.213788 0.00000 31.71554 0.000000 3.349758 11.631429 0.06147917 0.0000000 0.1882333 0.1882333 1.268288e-40 1.268288e-40 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000117118 E001 0.000000       1 17018722 17018723 2 -      
ENSG00000117118 E002 1.796445 0.1317384703 0.359675554 0.59555942 1 17018724 17018751 28 - 0.353 0.544 0.998
ENSG00000117118 E003 514.099829 0.0009858858 0.515283789 0.71186249 1 17018752 17018958 207 - 2.671 2.688 0.055
ENSG00000117118 E004 643.758963 0.0001679966 0.001109438 0.01074224 1 17022608 17022730 123 - 2.757 2.809 0.170
ENSG00000117118 E005 8.222696 0.0368582681 0.316720720 0.55669712 1 17022731 17023036 306 - 0.878 1.020 0.533
ENSG00000117118 E006 3.332882 0.0071902118 0.432486358 0.65311574 1 17023094 17023160 67 - 0.572 0.695 0.535
ENSG00000117118 E007 758.562790 0.0006297008 0.468591216 0.67908797 1 17023973 17024074 102 - 2.839 2.854 0.050
ENSG00000117118 E008 15.214114 0.0050766171 0.191593650 0.41889354 1 17027656 17027748 93 - 1.208 1.076 -0.472
ENSG00000117118 E009 920.609042 0.0001952353 0.409273654 0.63548911 1 17027749 17027865 117 - 2.929 2.921 -0.029
ENSG00000117118 E010 712.824230 0.0001616114 0.001648028 0.01465380 1 17028600 17028653 54 - 2.829 2.785 -0.148
ENSG00000117118 E011 776.268614 0.0002861817 0.003005440 0.02342756 1 17028654 17028736 83 - 2.866 2.824 -0.142
ENSG00000117118 E012 3.322589 0.1613313173 0.725460246 0.85142735 1 17032530 17033059 530 - 0.576 0.655 0.350
ENSG00000117118 E013 676.958131 0.0001147733 0.348298731 0.58575353 1 17033060 17033145 86 - 2.798 2.786 -0.040
ENSG00000117118 E014 613.484032 0.0004743342 0.171998529 0.39321701 1 17044761 17044888 128 - 2.743 2.769 0.084
ENSG00000117118 E015 0.000000       1 17045141 17045219 79 -      
ENSG00000117118 E016 0.000000       1 17052255 17052488 234 -      
ENSG00000117118 E017 335.579129 0.0021380858 0.042673287 0.16171447 1 17053948 17054149 202 - 2.470 2.531 0.202