ENSG00000116771

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375826 ENSG00000116771 HEK293_OSMI2_6hA HEK293_TMG_6hB AGMAT protein_coding protein_coding 2.160855 2.293133 1.16377 0.4868977 0.1192804 -0.9724475 0.9455328 0.6718898 0.6977713 0.05971112 0.05978327 0.05374465 0.4776667 0.3080667 0.6027333 0.2946667 0.03097987 0.03097987 FALSE TRUE
MSTRG.361.1 ENSG00000116771 HEK293_OSMI2_6hA HEK293_TMG_6hB AGMAT protein_coding   2.160855 2.293133 1.16377 0.4868977 0.1192804 -0.9724475 1.1358061 1.5815721 0.4149736 0.43688634 0.09467607 -1.90500756 0.4761333 0.6715333 0.3536000 -0.3179333 0.06038198 0.03097987 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000116771 E001 0.1779838 0.106161085 0.242843599   1 15529041 15529089 49 - 0.165 0.000 -10.089
ENSG00000116771 E002 2.4970933 0.094624497 0.308374092 0.54900628 1 15570362 15571698 1337 - 0.670 0.445 -1.040
ENSG00000116771 E003 11.2975329 0.004218912 0.060655413 0.20428267 1 15571699 15572914 1216 - 1.184 0.992 -0.698
ENSG00000116771 E004 20.4663565 0.016919865 0.006735601 0.04339872 1 15572915 15573587 673 - 1.449 1.214 -0.821
ENSG00000116771 E005 18.6190151 0.002397173 0.042474843 0.16117093 1 15573588 15573724 137 - 1.372 1.208 -0.576
ENSG00000116771 E006 15.3829188 0.033989193 0.600935700 0.77082741 1 15574757 15574841 85 - 1.166 1.202 0.127
ENSG00000116771 E007 16.7322107 0.003052964 0.040336506 0.15557554 1 15577685 15577864 180 - 1.098 1.270 0.612
ENSG00000116771 E008 19.4899029 0.003038917 0.023077443 0.10619999 1 15578859 15579054 196 - 1.143 1.325 0.643
ENSG00000116771 E009 11.1542403 0.003565781 0.112720551 0.30369562 1 15580094 15580142 49 - 0.946 1.105 0.582
ENSG00000116771 E010 19.6224469 0.005499433 0.327829443 0.56689220 1 15583193 15583395 203 - 1.222 1.298 0.266
ENSG00000116771 E011 13.2266099 0.012549073 0.365893219 0.60067239 1 15584696 15585051 356 - 1.047 1.140 0.338