ENSG00000116539

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368346 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding protein_coding 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.8338371 0.3434742 1.3184949 0.1789104 0.08486056 1.9101158 0.08716667 0.02410000 0.11396667 0.08986667 1.948356e-01 4.058342e-17 FALSE TRUE
ENST00000384987 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding miRNA 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.3320807 0.0000000 0.9553664 0.0000000 0.95536644 6.5930048 0.02508333 0.00000000 0.07760000 0.07760000 8.934294e-01 4.058342e-17   FALSE
ENST00000478837 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding retained_intron 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.5097958 1.3135574 0.2531710 0.6068660 0.03563965 -2.3303482 0.05312500 0.11640000 0.02213333 -0.09426667 4.461222e-01 4.058342e-17 FALSE TRUE
ENST00000492987 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding nonsense_mediated_decay 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.5388969 1.0077501 0.6147183 0.1850570 0.14108028 -0.7041057 0.05453750 0.08466667 0.05323333 -0.03143333 5.859366e-01 4.058342e-17 TRUE TRUE
ENST00000677825 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding protein_coding 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.5637970 0.0000000 1.1277085 0.0000000 0.56678457 6.8299872 0.05340833 0.00000000 0.09493333 0.09493333 2.955538e-01 4.058342e-17 FALSE TRUE
ENST00000677992 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding protein_coding 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 2.8343773 5.6410192 0.9210958 1.8666589 0.30103516 -2.6015095 0.30299583 0.42916667 0.08123333 -0.34793333 1.571118e-03 4.058342e-17 FALSE TRUE
ENST00000678699 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding nonsense_mediated_decay 9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 1.3136306 2.3618717 1.8827322 0.3651082 0.95470789 -0.3255557 0.13649167 0.19816667 0.16863333 -0.02953333 9.135927e-01 4.058342e-17 TRUE TRUE
MSTRG.2319.1 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding   9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 0.5399722 0.4259612 0.5102626 0.2132247 0.25632744 0.2550402 0.05664167 0.02923333 0.04296667 0.01373333 9.855366e-01 4.058342e-17 FALSE TRUE
MSTRG.2319.14 ENSG00000116539 HEK293_OSMI2_6hA HEK293_TMG_6hB ASH1L protein_coding   9.65624 12.29972 11.56377 2.622159 0.3969266 -0.08893906 1.3099652 0.0000000 2.9021902 0.0000000 0.33556559 8.1859608 0.13351667 0.00000000 0.25350000 0.25350000 4.058342e-17 4.058342e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000116539 E001 0.1779838 0.0359173865 5.339845e-01   1 155335223 155335267 45 - 0.121 0.000 -10.929
ENSG00000116539 E002 0.8757361 0.0184293008 5.968548e-01   1 155335268 155335286 19 - 0.215 0.296 0.614
ENSG00000116539 E003 1.5429409 0.0375683272 8.509940e-02 2.548049e-01 1 155335287 155335300 14 - 0.215 0.517 1.838
ENSG00000116539 E004 419.8272621 0.0096018031 3.364741e-10 2.136114e-08 1 155335301 155337172 1872 - 2.462 2.741 0.930
ENSG00000116539 E005 249.4563405 0.0022190867 2.542415e-09 1.365898e-07 1 155337173 155337751 579 - 2.310 2.469 0.528
ENSG00000116539 E006 182.1921878 0.0002528066 1.416325e-05 2.834369e-04 1 155338089 155338390 302 - 2.219 2.306 0.291
ENSG00000116539 E007 2.2958325 0.0100667900 4.994335e-01 7.003989e-01 1 155338391 155338405 15 - 0.465 0.557 0.443
ENSG00000116539 E008 73.3929040 0.0005908611 3.118568e-02 1.308905e-01 1 155339328 155339368 41 - 1.837 1.900 0.213
ENSG00000116539 E009 121.7527618 0.0066855339 5.342606e-02 1.877141e-01 1 155341936 155342102 167 - 2.050 2.124 0.248
ENSG00000116539 E010 3.4295282 0.1189287746 1.160931e-01 3.093236e-01 1 155343041 155343313 273 - 0.464 0.757 1.300
ENSG00000116539 E011 101.4745024 0.0012322042 3.533259e-02 1.424400e-01 1 155343314 155343450 137 - 1.980 2.035 0.182
ENSG00000116539 E012 58.1905439 0.0007065582 1.443961e-01 3.539825e-01 1 155343451 155343486 36 - 1.748 1.790 0.141
ENSG00000116539 E013 0.6653672 0.2260357748 9.250409e-01   1 155343592 155343603 12 - 0.215 0.220 0.044
ENSG00000116539 E014 67.0093456 0.0006450494 5.634697e-02 1.944368e-01 1 155343604 155343655 52 - 1.801 1.858 0.190
ENSG00000116539 E015 79.6026154 0.0007667215 1.358479e-01 3.411141e-01 1 155343656 155343742 87 - 1.887 1.920 0.112
ENSG00000116539 E016 67.1395109 0.0097558267 9.698119e-02 2.764661e-01 1 155344183 155344273 91 - 1.793 1.866 0.245
ENSG00000116539 E017 2.6612357 0.2680415198 3.532168e-01 5.901700e-01 1 155344274 155344591 318 - 0.678 0.421 -1.198
ENSG00000116539 E018 2.5651692 0.0088578107 1.714688e-02 8.599163e-02 1 155345963 155346207 245 - 0.712 0.296 -2.088
ENSG00000116539 E019 0.8048655 0.0182516324 2.203632e-02   1 155346350 155346382 33 - 0.415 0.000 -13.249
ENSG00000116539 E020 68.0914914 0.0009925584 2.394052e-02 1.089169e-01 1 155346383 155346469 87 - 1.799 1.871 0.244
ENSG00000116539 E021 135.7525008 0.0003188310 1.185924e-03 1.132463e-02 1 155347656 155347901 246 - 2.100 2.172 0.241
ENSG00000116539 E022 40.6155739 0.0009543585 3.306212e-03 2.523578e-02 1 155347902 155347904 3 - 1.546 1.683 0.467
ENSG00000116539 E023 87.4417750 0.0004633798 2.433841e-03 1.990475e-02 1 155349327 155349459 133 - 1.903 1.991 0.298
ENSG00000116539 E024 48.9886273 0.0008282012 4.876072e-04 5.563112e-03 1 155349542 155349596 55 - 1.622 1.773 0.511
ENSG00000116539 E025 66.8326673 0.0009593692 1.447347e-04 2.036394e-03 1 155352706 155352858 153 - 1.760 1.902 0.478
ENSG00000116539 E026 84.1148553 0.0019134564 2.229885e-02 1.035962e-01 1 155354473 155354630 158 - 1.895 1.965 0.233
ENSG00000116539 E027 77.1098468 0.0031889880 6.274410e-01 7.888608e-01 1 155357316 155357410 95 - 1.899 1.895 -0.012
ENSG00000116539 E028 91.8811708 0.0004350794 3.789722e-02 1.493507e-01 1 155357585 155357749 165 - 2.019 1.916 -0.347
ENSG00000116539 E029 67.7233079 0.0010227610 2.579006e-02 1.146440e-01 1 155360301 155360409 109 - 1.897 1.773 -0.421
ENSG00000116539 E030 0.1723744 0.0472169171 4.453515e-01   1 155370147 155370503 357 - 0.000 0.123 10.950
ENSG00000116539 E031 70.4496805 0.0005637053 1.056752e-04 1.566795e-03 1 155370504 155370650 147 - 1.941 1.749 -0.648
ENSG00000116539 E032 94.6871715 0.0007785988 2.531738e-06 6.412338e-05 1 155370777 155370983 207 - 2.074 1.869 -0.689
ENSG00000116539 E033 65.7437983 0.0007685254 1.248360e-02 6.836731e-02 1 155378281 155378389 109 - 1.890 1.753 -0.462
ENSG00000116539 E034 42.2961817 0.0009859773 6.095329e-01 7.765977e-01 1 155378503 155378548 46 - 1.663 1.610 -0.181
ENSG00000116539 E035 47.4601478 0.0009900385 7.766116e-01 8.826807e-01 1 155380043 155380116 74 - 1.703 1.662 -0.139
ENSG00000116539 E036 0.1614157 0.0330910762 5.345760e-01   1 155380117 155380131 15 - 0.120 0.000 -10.927
ENSG00000116539 E037 49.4949952 0.0082890265 8.261604e-01 9.115607e-01 1 155395459 155395553 95 - 1.723 1.678 -0.152
ENSG00000116539 E038 55.5074353 0.0007334477 2.486798e-01 4.864449e-01 1 155415744 155415923 180 - 1.795 1.714 -0.272
ENSG00000116539 E039 146.0614746 0.0026939741 5.952694e-03 3.950693e-02 1 155438327 155439068 742 - 2.231 2.100 -0.437
ENSG00000116539 E040 0.0000000       1 155440490 155440502 13 -      
ENSG00000116539 E041 1.3058530 0.0304016502 5.711445e-01   1 155440503 155440593 91 - 0.414 0.297 -0.703
ENSG00000116539 E042 42.6286307 0.0087177152 2.347259e-02 1.074512e-01 1 155459797 155459898 102 - 1.722 1.537 -0.630
ENSG00000116539 E043 1.0067919 0.0172671820 9.204178e-01   1 155462453 155462599 147 - 0.292 0.296 0.028
ENSG00000116539 E044 579.2895582 0.0184450110 3.564846e-09 1.847130e-07 1 155477886 155482449 4564 - 2.916 2.529 -1.288
ENSG00000116539 E045 0.0000000       1 155520225 155520356 132 -      
ENSG00000116539 E046 66.2284967 0.0006080718 7.255568e-27 4.496943e-24 1 155521100 155521618 519 - 2.022 1.486 -1.813
ENSG00000116539 E047 10.6204759 0.1895051561 6.365555e-01 7.947173e-01 1 155562153 155562291 139 - 1.157 0.964 -0.706
ENSG00000116539 E048 5.4325017 0.0790149474 8.918202e-01 9.475244e-01 1 155562292 155562295 4 - 0.848 0.786 -0.241
ENSG00000116539 E049 9.7433099 0.0290071423 3.794485e-01 6.117712e-01 1 155562296 155562778 483 - 1.106 0.959 -0.535
ENSG00000116539 E050 2.8635423 0.0311349033 6.144381e-01 7.798459e-01 1 155562779 155563072 294 - 0.654 0.554 -0.445
ENSG00000116539 E051 0.9287192 0.0160919870 1.076457e-02   1 155563073 155564202 1130 - 0.465 0.000 -13.510