ENSG00000115896

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000428675 ENSG00000115896 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL1 protein_coding protein_coding 0.2810131 0.1058093 0.4561401 0.0329656 0.01347242 2.009012 0.05411456 0.05603197 0.06446574 0.03479899 0.06446574 0.1734121 0.1467042 0.4374667 0.14023333 -0.29723333 0.73679795 0.03187732 FALSE TRUE
ENST00000435320 ENSG00000115896 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL1 protein_coding nonsense_mediated_decay 0.2810131 0.1058093 0.4561401 0.0329656 0.01347242 2.009012 0.03304311 0.00000000 0.01252953 0.00000000 0.01252953 1.1718173 0.1215458 0.0000000 0.02623333 0.02623333 1.00000000 0.03187732 FALSE TRUE
ENST00000437704 ENSG00000115896 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL1 protein_coding protein_coding 0.2810131 0.1058093 0.4561401 0.0329656 0.01347242 2.009012 0.07385068 0.04977736 0.12424861 0.03087638 0.06352046 1.1672360 0.4944208 0.5625333 0.27590000 -0.28663333 0.83323081 0.03187732 FALSE TRUE
ENST00000487695 ENSG00000115896 HEK293_OSMI2_6hA HEK293_TMG_6hB PLCL1 protein_coding protein_coding 0.2810131 0.1058093 0.4561401 0.0329656 0.01347242 2.009012 0.11832454 0.00000000 0.25489621 0.00000000 0.01674415 4.7273553 0.2330625 0.0000000 0.55763333 0.55763333 0.03187732 0.03187732 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000115896 E001 0.2922838 0.027442404 0.512656503   2 197804593 197804701 109 + 0.083 0.174 1.233
ENSG00000115896 E002 0.9487176 0.184950153 0.354097768   2 197804702 197805339 638 + 0.213 0.393 1.218
ENSG00000115896 E003 0.0000000       2 197810258 197810322 65 +      
ENSG00000115896 E004 0.1426347 0.039668674 1.000000000   2 198001952 198002008 57 + 0.083 0.000 -9.282
ENSG00000115896 E005 0.0000000       2 198083749 198083757 9 +      
ENSG00000115896 E006 16.8374723 0.003720938 0.007265128 0.04590658 2 198083758 198086232 2475 + 1.266 1.057 -0.745
ENSG00000115896 E007 2.8512806 0.008546582 0.179937456 0.40389012 2 198088858 198089061 204 + 0.492 0.695 0.913
ENSG00000115896 E008 1.7435479 0.011366685 0.624801570 0.78721326 2 198101285 198101360 76 + 0.393 0.474 0.429
ENSG00000115896 E009 2.3151316 0.010351232 0.799058838 0.89572378 2 198103827 198103936 110 + 0.520 0.474 -0.226
ENSG00000115896 E010 2.9759575 0.008053788 0.041547589 0.15876280 2 198146780 198146932 153 + 0.660 0.299 -1.853
ENSG00000115896 E011 11.4696590 0.003390005 0.008245516 0.05045342 2 198146933 198149374 2442 + 0.983 1.200 0.785
ENSG00000115896 E012 0.1614157 0.041951196 1.000000000   2 198149375 198149418 44 + 0.083 0.000 -9.282
ENSG00000115896 E013 0.2922838 0.027442404 0.512656503   2 198149419 198149863 445 + 0.083 0.174 1.233
ENSG00000115896 E014 0.0000000       2 198149864 198149884 21 +      
ENSG00000115896 E015 0.0000000       2 198346829 198346907 79 +      
ENSG00000115896 E016 0.0000000       2 198355295 198355407 113 +      
ENSG00000115896 E017 0.0000000       2 198355408 198355486 79 +      
ENSG00000115896 E018 0.0000000       2 198387853 198388005 153 +      
ENSG00000115896 E019 0.0000000       2 198571354 198571477 124 +      
ENSG00000115896 E020 0.0000000       2 198571856 198572581 726 +