ENSG00000115866

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264161 ENSG00000115866 HEK293_OSMI2_6hA HEK293_TMG_6hB DARS1 protein_coding protein_coding 70.96474 17.51033 115.2747 1.121174 8.882955 2.7181 28.963213 10.723777 43.64572 2.0768361 0.5607049 2.024013 0.50036250 0.60176667 0.38266667 -0.21910000 2.816341e-01 5.029666e-08 FALSE TRUE
ENST00000422708 ENSG00000115866 HEK293_OSMI2_6hA HEK293_TMG_6hB DARS1 protein_coding protein_coding 70.96474 17.51033 115.2747 1.121174 8.882955 2.7181 19.713222 5.320123 31.98938 0.3314187 2.3366966 2.585803 0.28545833 0.30483333 0.27793333 -0.02690000 8.215553e-01 5.029666e-08 FALSE TRUE
ENST00000435076 ENSG00000115866 HEK293_OSMI2_6hA HEK293_TMG_6hB DARS1 protein_coding nonsense_mediated_decay 70.96474 17.51033 115.2747 1.121174 8.882955 2.7181 4.216717 0.000000 6.44832 0.0000000 1.9611017 9.335015 0.03418333 0.00000000 0.05396667 0.05396667 5.029666e-08 5.029666e-08 FALSE TRUE
ENST00000441323 ENSG00000115866 HEK293_OSMI2_6hA HEK293_TMG_6hB DARS1 protein_coding protein_coding 70.96474 17.51033 115.2747 1.121174 8.882955 2.7181 16.921560 1.120285 30.95911 1.1202849 4.1039147 4.776071 0.15643750 0.07313333 0.26620000 0.19306667 2.134022e-01 5.029666e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000115866 E001 114.2401174 0.0366902917 6.510645e-03 0.0422571093 2 135905881 135906823 943 - 1.894 2.185 0.976
ENSG00000115866 E002 300.2082752 0.0166781986 6.859509e-03 0.0439884619 2 135906824 135907243 420 - 2.342 2.536 0.648
ENSG00000115866 E003 32.9112589 0.0048720915 4.372315e-05 0.0007425196 2 135907244 135907259 16 - 1.364 1.676 1.069
ENSG00000115866 E004 234.6954636 0.0002845006 4.335680e-03 0.0310216245 2 135907260 135907407 148 - 2.265 2.359 0.314
ENSG00000115866 E005 1.1855836 0.0150873068 6.478585e-01   2 135910848 135911138 291 - 0.299 0.213 -0.649
ENSG00000115866 E006 294.1940016 0.0015460550 8.048516e-01 0.8991133481 2 135911139 135911210 72 - 2.382 2.392 0.032
ENSG00000115866 E007 409.5751986 0.0004144439 8.153283e-02 0.2479034182 2 135911382 135911493 112 - 2.533 2.511 -0.076
ENSG00000115866 E008 1.6538261 0.0132101825 3.499484e-01 0.5871978054 2 135911494 135911617 124 - 0.396 0.213 -1.236
ENSG00000115866 E009 338.4766643 0.0016318085 9.816276e-02 0.2785707639 2 135912486 135912566 81 - 2.452 2.420 -0.107
ENSG00000115866 E010 282.5596052 0.0010541228 1.331426e-01 0.3367340182 2 135914469 135914511 43 - 2.373 2.345 -0.093
ENSG00000115866 E011 5.2287883 0.2275632771 3.967128e-01 0.6257301587 2 135914512 135914866 355 - 0.650 0.889 0.960
ENSG00000115866 E012 488.0452483 0.0019178457 5.516463e-01 0.7374466278 2 135916226 135916372 147 - 2.602 2.600 -0.007
ENSG00000115866 E013 465.9493865 0.0053457162 8.049477e-01 0.8991759053 2 135920453 135920600 148 - 2.581 2.582 0.004
ENSG00000115866 E014 368.0355360 0.0008820127 7.495303e-01 0.8664732605 2 135922784 135922918 135 - 2.475 2.499 0.082
ENSG00000115866 E015 209.4629091 0.0002365486 7.528178e-01 0.8684564484 2 135924387 135924417 31 - 2.235 2.242 0.022
ENSG00000115866 E016 186.0202459 0.0003393115 3.284714e-01 0.5674825457 2 135924418 135924436 19 - 2.188 2.172 -0.052
ENSG00000115866 E017 277.9570453 0.0010287036 4.932131e-01 0.6961138694 2 135924437 135924498 62 - 2.359 2.354 -0.017
ENSG00000115866 E018 315.8391993 0.0001866729 1.129919e-01 0.3041429278 2 135932783 135932842 60 - 2.420 2.397 -0.075
ENSG00000115866 E019 0.9584589 0.0154605654 7.966505e-01   2 135933909 135933909 1 - 0.261 0.213 -0.385
ENSG00000115866 E020 281.9414435 0.0001995170 4.557436e-01 0.6700559821 2 135933910 135933990 81 - 2.367 2.364 -0.010
ENSG00000115866 E021 0.4842470 0.6819892899 1.000000e+00   2 135943179 135943377 199 - 0.176 0.000 -8.970
ENSG00000115866 E022 143.2226916 0.0003167598 9.082791e-02 0.2652536771 2 135943378 135943385 8 - 2.083 2.037 -0.155
ENSG00000115866 E023 330.5168676 0.0001640419 1.170440e-02 0.0652531498 2 135943386 135943480 95 - 2.445 2.400 -0.148
ENSG00000115866 E024 336.0381388 0.0025756589 1.364835e-01 0.3421264381 2 135961396 135961498 103 - 2.450 2.413 -0.125
ENSG00000115866 E025 0.9121510 0.0299439497 2.002176e-01   2 135978987 135979273 287 - 0.298 0.000 -9.954
ENSG00000115866 E026 309.0638060 0.0155923439 5.446375e-01 0.7323646475 2 135979274 135979366 93 - 2.413 2.375 -0.125
ENSG00000115866 E027 0.0000000       2 135980431 135980551 121 -      
ENSG00000115866 E028 216.2662332 0.0783501126 8.143788e-01 0.9048694360 2 135983397 135983454 58 - 2.251 2.247 -0.014
ENSG00000115866 E029 186.5383654 0.8379544801 4.700215e-01 0.6801030335 2 135985403 135985626 224 - 2.158 2.277 0.399
ENSG00000115866 E030 0.0000000       2 135985794 135985812 19 -      
ENSG00000115866 E031 0.0000000       2 135985813 135985897 85 -      
ENSG00000115866 E032 0.0000000       2 135985930 135986100 171 -