ENSG00000115421

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000238714 ENSG00000115421 HEK293_OSMI2_6hA HEK293_TMG_6hB PAPOLG protein_coding protein_coding 4.755956 1.523183 10.05537 0.2785211 0.1479426 2.714799 3.1637105 1.34977643 5.487274 0.27900358 0.1578749 2.015347 0.75176250 0.87623333 0.5460333 -0.3302000 3.247980e-05 2.38273e-06 FALSE TRUE
ENST00000470208 ENSG00000115421 HEK293_OSMI2_6hA HEK293_TMG_6hB PAPOLG protein_coding retained_intron 4.755956 1.523183 10.05537 0.2785211 0.1479426 2.714799 0.5878763 0.03106268 1.552580 0.03106268 0.1293279 5.249959 0.08634583 0.03143333 0.1546333 0.1232000 1.082232e-01 2.38273e-06 FALSE FALSE
ENST00000483370 ENSG00000115421 HEK293_OSMI2_6hA HEK293_TMG_6hB PAPOLG protein_coding processed_transcript 4.755956 1.523183 10.05537 0.2785211 0.1479426 2.714799 0.5722352 0.00000000 2.067792 0.00000000 0.2661889 7.698907 0.06370417 0.00000000 0.2052667 0.2052667 2.382730e-06 2.38273e-06 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000115421 E001 0.6316833 0.0268939592 7.553927e-01   2 60756240 60756250 11 + 0.161 0.229 0.622
ENSG00000115421 E002 1.1185192 0.1586499877 6.169736e-01   2 60756251 60756252 2 + 0.246 0.373 0.839
ENSG00000115421 E003 2.4799439 0.0093763161 9.922009e-01 9.978086e-01 2 60756253 60756262 10 + 0.470 0.488 0.094
ENSG00000115421 E004 2.8005624 0.0110717974 8.127456e-01 9.038394e-01 2 60756263 60756265 3 + 0.512 0.488 -0.113
ENSG00000115421 E005 2.8005624 0.0110717974 8.127456e-01 9.038394e-01 2 60756266 60756267 2 + 0.512 0.488 -0.113
ENSG00000115421 E006 29.6826024 0.0015756598 4.412691e-01 6.596391e-01 2 60756268 60756349 82 + 1.387 1.352 -0.120
ENSG00000115421 E007 42.1156007 0.0011640866 4.442060e-01 6.615429e-01 2 60756350 60756495 146 + 1.533 1.508 -0.084
ENSG00000115421 E008 21.9975569 0.0504305728 4.916913e-01 6.951302e-01 2 60760134 60760134 1 + 1.271 1.202 -0.243
ENSG00000115421 E009 51.0006889 0.0103424051 1.160699e-01 3.092847e-01 2 60760135 60760295 161 + 1.626 1.525 -0.343
ENSG00000115421 E010 32.4573760 0.0012668210 4.665977e-01 6.777791e-01 2 60761741 60761807 67 + 1.420 1.391 -0.100
ENSG00000115421 E011 33.3667142 0.0011449709 2.508937e-01 4.889491e-01 2 60768470 60768551 82 + 1.440 1.379 -0.211
ENSG00000115421 E012 29.5068651 0.0016063262 3.075542e-01 5.482060e-01 2 60768781 60768890 110 + 1.382 1.325 -0.198
ENSG00000115421 E013 26.8719954 0.0014672941 3.438762e-01 5.815374e-01 2 60770458 60770511 54 + 1.348 1.295 -0.184
ENSG00000115421 E014 31.2082126 0.0011847222 6.530953e-02 2.145978e-01 2 60771519 60771630 112 + 1.420 1.295 -0.434
ENSG00000115421 E015 33.4649778 0.0010649424 7.549107e-03 4.725503e-02 2 60775034 60775123 90 + 1.458 1.264 -0.676
ENSG00000115421 E016 0.0000000       2 60779554 60779636 83 +      
ENSG00000115421 E017 38.7359103 0.0009126071 4.980969e-02 1.791746e-01 2 60779637 60779775 139 + 1.509 1.391 -0.408
ENSG00000115421 E018 31.9212219 0.0012177496 1.236519e-01 3.214926e-01 2 60780707 60780779 73 + 1.423 1.325 -0.339
ENSG00000115421 E019 37.7401869 0.0011882107 3.587965e-02 1.438872e-01 2 60781885 60781974 90 + 1.499 1.366 -0.461
ENSG00000115421 E020 28.3711394 0.0020906283 9.129622e-03 5.440152e-02 2 60781975 60782005 31 + 1.387 1.173 -0.753
ENSG00000115421 E021 33.2016520 0.0070124845 3.649926e-02 1.455996e-01 2 60782686 60782770 85 + 1.446 1.280 -0.575
ENSG00000115421 E022 24.8426800 0.0053774613 3.966876e-03 2.896619e-02 2 60783156 60783209 54 + 1.337 1.058 -0.989
ENSG00000115421 E023 48.4550424 0.0007935232 3.713512e-03 2.754848e-02 2 60786947 60787066 120 + 1.610 1.438 -0.590
ENSG00000115421 E024 56.8353663 0.0008509552 1.370409e-01 3.430124e-01 2 60787511 60787620 110 + 1.662 1.601 -0.210
ENSG00000115421 E025 1.1552237 0.0153905852 7.732967e-01   2 60791481 60791760 280 + 0.279 0.229 -0.373
ENSG00000115421 E026 60.0973626 0.0007908808 4.469468e-01 6.633965e-01 2 60791761 60791882 122 + 1.679 1.664 -0.052
ENSG00000115421 E027 61.8574944 0.0006433236 9.365739e-01 9.704995e-01 2 60792129 60792289 161 + 1.680 1.713 0.112
ENSG00000115421 E028 40.8106529 0.0008590167 7.060810e-01 8.394035e-01 2 60793627 60793715 89 + 1.499 1.553 0.184
ENSG00000115421 E029 65.7815728 0.0100843458 2.286692e-01 4.636810e-01 2 60793971 60794191 221 + 1.722 1.661 -0.205
ENSG00000115421 E030 4.0556270 0.0148243737 3.034865e-02 1.284027e-01 2 60794192 60794709 518 + 0.663 0.229 -2.378
ENSG00000115421 E031 37.9618842 0.0013020653 9.462674e-01 9.754686e-01 2 60794710 60794775 66 + 1.476 1.499 0.080
ENSG00000115421 E032 3.8916047 0.0518969009 5.110878e-01 7.088272e-01 2 60794776 60794963 188 + 0.582 0.714 0.565
ENSG00000115421 E033 46.0587427 0.0007851763 3.741849e-01 6.074660e-01 2 60794964 60795020 57 + 1.546 1.630 0.287
ENSG00000115421 E034 0.7201878 0.0596067810 1.000000e+00   2 60795290 60795336 47 + 0.203 0.228 0.215
ENSG00000115421 E035 364.3066058 0.0026017860 3.915725e-16 6.926877e-14 2 60797062 60802086 5025 + 2.396 2.628 0.775