ENSG00000114742

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302313 ENSG00000114742 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR48 protein_coding protein_coding 25.68467 9.146026 48.89927 1.650066 2.444143 2.417314 18.186353 8.0560670 31.785783 1.824357 1.3158991 1.978898 0.77568750 0.86153333 0.65116667 -0.21036667 1.121600e-01 5.974427e-18 FALSE TRUE
ENST00000413099 ENSG00000114742 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR48 protein_coding nonsense_mediated_decay 25.68467 9.146026 48.89927 1.650066 2.444143 2.417314 2.378041 0.0000000 6.349908 0.000000 0.2173931 9.312862 0.05582083 0.00000000 0.13023333 0.13023333 5.974427e-18 5.974427e-18 FALSE TRUE
MSTRG.22757.1 ENSG00000114742 HEK293_OSMI2_6hA HEK293_TMG_6hB WDR48 protein_coding   25.68467 9.146026 48.89927 1.650066 2.444143 2.417314 2.302923 0.4779919 4.698114 0.273280 0.3014666 3.270220 0.06894167 0.04413333 0.09596667 0.05183333 6.250064e-01 5.974427e-18 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000114742 E001 3.2009235 0.2120053281 7.681302e-01 8.775036e-01 3 39051956 39052012 57 + 0.553 0.493 -0.285
ENSG00000114742 E002 6.1427697 0.5316142745 7.248911e-01 8.510440e-01 3 39052013 39052015 3 + 0.779 0.640 -0.578
ENSG00000114742 E003 6.4577787 0.3950526290 9.209279e-01 9.627076e-01 3 39052016 39052018 3 + 0.790 0.708 -0.331
ENSG00000114742 E004 7.4184504 0.4276110300 9.862601e-01 9.949663e-01 3 39052019 39052019 1 + 0.838 0.765 -0.287
ENSG00000114742 E005 60.0379299 0.9803060266 6.720122e-01 8.176004e-01 3 39052020 39052073 54 + 1.664 1.686 0.075
ENSG00000114742 E006 1.7105290 0.3830909129 4.668472e-01 6.779058e-01 3 39052301 39052601 301 + 0.311 0.507 1.079
ENSG00000114742 E007 147.1702698 0.0240085372 7.208389e-02 2.289843e-01 3 39063050 39063190 141 + 2.070 1.963 -0.359
ENSG00000114742 E008 0.3040503 0.0244411696 1.000000e+00   3 39065791 39065810 20 + 0.111 0.001 -7.376
ENSG00000114742 E009 155.7297064 0.0002951298 7.846905e-08 2.987859e-06 3 39065811 39065889 79 + 2.103 1.964 -0.466
ENSG00000114742 E010 164.9937049 0.0036461578 1.204404e-03 1.146692e-02 3 39066548 39066630 83 + 2.116 2.036 -0.270
ENSG00000114742 E011 0.4702677 0.0216879135 5.522881e-01   3 39066727 39066745 19 + 0.112 0.233 1.276
ENSG00000114742 E012 214.9501884 0.0071181734 5.520710e-02 1.918012e-01 3 39066746 39066875 130 + 2.221 2.198 -0.077
ENSG00000114742 E013 200.3417289 0.0085729169 1.219454e-02 6.723392e-02 3 39068771 39068859 89 + 2.197 2.131 -0.218
ENSG00000114742 E014 209.9820154 0.0073943818 4.524092e-03 3.207011e-02 3 39069643 39069744 102 + 2.219 2.146 -0.245
ENSG00000114742 E015 233.7973095 0.0034302470 2.963215e-04 3.693644e-03 3 39074726 39074950 225 + 2.265 2.191 -0.248
ENSG00000114742 E016 128.9597060 0.0023355716 7.869939e-03 4.872035e-02 3 39077139 39077213 75 + 2.004 1.953 -0.171
ENSG00000114742 E017 1.7555020 0.0122849117 9.230068e-01 9.638070e-01 3 39078011 39078136 126 + 0.365 0.384 0.111
ENSG00000114742 E018 97.0032834 0.0082358866 1.198172e-01 3.155613e-01 3 39078137 39078160 24 + 1.877 1.849 -0.093
ENSG00000114742 E019 177.8315758 0.0063232675 1.339331e-02 7.200038e-02 3 39078161 39078239 79 + 2.142 2.087 -0.183
ENSG00000114742 E020 207.6718704 0.0071558422 4.329606e-03 3.098730e-02 3 39079711 39079808 98 + 2.212 2.141 -0.237
ENSG00000114742 E021 205.3008146 0.0085816001 8.454845e-03 5.140347e-02 3 39084155 39084262 108 + 2.206 2.136 -0.236
ENSG00000114742 E022 140.4186952 0.0112305291 3.591655e-01 5.951872e-01 3 39084645 39084741 97 + 2.028 2.045 0.056
ENSG00000114742 E023 171.8928465 0.0127491761 6.894659e-01 8.286770e-01 3 39085515 39085610 96 + 2.111 2.153 0.139
ENSG00000114742 E024 3.7381785 0.0400888270 2.575872e-01 4.963377e-01 3 39086301 39086384 84 + 0.526 0.776 1.076
ENSG00000114742 E025 5.6903957 0.2503777412 7.988298e-01 8.956207e-01 3 39087818 39088127 310 + 0.720 0.734 0.055
ENSG00000114742 E026 185.7930302 0.0082206959 8.946405e-01 9.490439e-01 3 39088128 39088233 106 + 2.141 2.204 0.211
ENSG00000114742 E027 176.3244461 0.0082794081 1.554827e-01 3.701484e-01 3 39089231 39089318 88 + 2.131 2.129 -0.007
ENSG00000114742 E028 20.8688883 0.1491130371 5.783400e-01 7.560218e-01 3 39089568 39091624 2057 + 1.233 1.188 -0.159
ENSG00000114742 E029 158.1172183 0.0092093588 8.983048e-01 9.509470e-01 3 39091625 39091701 77 + 2.067 2.148 0.272
ENSG00000114742 E030 235.4687171 0.0081493079 5.109104e-01 7.086480e-01 3 39093874 39094066 193 + 2.234 2.337 0.343
ENSG00000114742 E031 1.6800517 0.0588618034 2.118959e-01 4.439662e-01 3 39094067 39094647 581 + 0.305 0.583 1.472
ENSG00000114742 E032 484.1075372 0.0024825782 1.792809e-06 4.732788e-05 3 39094648 39095414 767 + 2.526 2.726 0.665
ENSG00000114742 E033 68.4175898 0.9639050993 3.402362e-01 5.781720e-01 3 39095415 39095480 66 + 1.657 1.949 0.986
ENSG00000114742 E034 445.8904714 1.8133023456 3.490275e-01 5.864191e-01 3 39095481 39096671 1191 + 2.411 2.861 1.496