ENSG00000114686

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264995 ENSG00000114686 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL3 protein_coding protein_coding 116.9401 30.92034 193.24 3.437547 9.630623 2.643374 80.64969 26.457511 124.44286 4.0974668 5.793962 2.233305 0.73775417 0.84653333 0.6443333 -0.2022 6.345602e-02 7.117235e-23 FALSE TRUE
ENST00000511168 ENSG00000114686 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL3 protein_coding protein_coding 116.9401 30.92034 193.24 3.437547 9.630623 2.643374 18.58803 2.372992 40.13675 0.6644451 4.468328 4.074437 0.14515833 0.08373333 0.2064333 0.1227 1.553042e-01 7.117235e-23 FALSE TRUE
MSTRG.23712.1 ENSG00000114686 HEK293_OSMI2_6hA HEK293_TMG_6hB MRPL3 protein_coding   116.9401 30.92034 193.24 3.437547 9.630623 2.643374 12.61351 0.000000 21.76214 0.0000000 1.668573 11.088268 0.06589167 0.00000000 0.1136000 0.1136 7.117235e-23 7.117235e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000114686 E001 0.4341107 0.0244411696 0.003745400   3 131432106 131432175 70 - 0.000 0.485 13.762
ENSG00000114686 E002 0.1308682 0.0310855644 0.104929444   3 131442076 131442145 70 - 0.000 0.226 12.184
ENSG00000114686 E003 0.0000000       3 131442396 131442450 55 -      
ENSG00000114686 E004 159.6392587 1.3775964220 0.435172258 0.65509217 3 131462212 131462435 224 - 2.044 2.274 0.771
ENSG00000114686 E005 293.4544636 1.5758704104 0.454856437 0.66937566 3 131462436 131462553 118 - 2.307 2.536 0.763
ENSG00000114686 E006 193.3524257 1.4404312533 0.457767929 0.67161266 3 131462554 131462555 2 - 2.134 2.337 0.678
ENSG00000114686 E007 914.2667612 1.9464412972 0.536915173 0.72697582 3 131462556 131462875 320 - 2.813 2.990 0.588
ENSG00000114686 E008 522.7932656 0.0160576603 0.243356174 0.48042729 3 131468091 131468168 78 - 2.592 2.685 0.310
ENSG00000114686 E009 492.9138820 0.0036926500 0.499292388 0.70032579 3 131469696 131469731 36 - 2.581 2.612 0.103
ENSG00000114686 E010 546.0297770 0.0093190812 0.176856155 0.39954029 3 131469732 131469773 42 - 2.630 2.625 -0.017
ENSG00000114686 E011 406.8309879 0.0140958117 0.312393220 0.55270407 3 131471171 131471172 2 - 2.501 2.503 0.005
ENSG00000114686 E012 724.2503869 0.0202818203 0.241958641 0.47874637 3 131471173 131471279 107 - 2.753 2.739 -0.047
ENSG00000114686 E013 3.4317252 0.0949464405 0.039129574 0.15249103 3 131471280 131471334 55 - 0.449 0.892 1.909
ENSG00000114686 E014 2.4267726 0.1283206847 0.547101132 0.73422544 3 131487150 131487679 530 - 0.429 0.572 0.699
ENSG00000114686 E015 356.5105630 0.0300292815 0.219425831 0.45273856 3 131487680 131487680 1 - 2.451 2.411 -0.133
ENSG00000114686 E016 579.7949691 0.0343954161 0.119096570 0.31438999 3 131487681 131487740 60 - 2.667 2.596 -0.234
ENSG00000114686 E017 0.4764247 0.0217681645 0.550981151   3 131488671 131488800 130 - 0.115 0.227 1.178
ENSG00000114686 E018 649.2000611 0.0309143693 0.111890746 0.30225208 3 131489981 131490080 100 - 2.715 2.649 -0.220
ENSG00000114686 E019 4.7012311 0.1308072199 0.061531040 0.20623652 3 131497980 131498178 199 - 0.535 0.979 1.812
ENSG00000114686 E020 643.1478652 0.0316644666 0.070055090 0.22492089 3 131498179 131498246 68 - 2.715 2.624 -0.300
ENSG00000114686 E021 527.2401489 0.0330162980 0.048619458 0.17641603 3 131498247 131498277 31 - 2.632 2.521 -0.369
ENSG00000114686 E022 638.3099813 0.0256000810 0.034899705 0.14133705 3 131500430 131500521 92 - 2.713 2.614 -0.327
ENSG00000114686 E023 795.3514373 0.0003608219 0.008295762 0.05068578 3 131501531 131501715 185 - 2.797 2.796 0.000
ENSG00000114686 E024 5.0736903 0.0286632060 0.577654170 0.75557222 3 131501844 131501924 81 - 0.679 0.810 0.532
ENSG00000114686 E025 364.0729651 1.6462829274 0.471522600 0.68121219 3 131502730 131502782 53 - 2.404 2.619 0.716
ENSG00000114686 E026 260.8907225 1.5381844996 0.457750280 0.67161266 3 131502783 131502794 12 - 2.260 2.476 0.719
ENSG00000114686 E027 318.3689120 1.6029084779 0.464884360 0.67649891 3 131502795 131502848 54 - 2.345 2.563 0.725
ENSG00000114686 E028 318.0689676 1.6008816513 0.456056653 0.67030958 3 131502849 131502983 135 - 2.342 2.569 0.756