ENSG00000114520

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000251775 ENSG00000114520 HEK293_OSMI2_6hA HEK293_TMG_6hB SNX4 protein_coding protein_coding 17.58103 3.270015 28.16176 0.452941 0.7091687 3.102476 13.2345545 2.98714862 21.748175 0.49571012 0.5706954 2.859895 0.82554167 0.9062333 0.77236667 -0.13386667 0.061818569 0.009173587 FALSE TRUE
ENST00000471751 ENSG00000114520 HEK293_OSMI2_6hA HEK293_TMG_6hB SNX4 protein_coding nonsense_mediated_decay 17.58103 3.270015 28.16176 0.452941 0.7091687 3.102476 2.6956284 0.06886236 3.011350 0.06886236 0.2704151 5.259713 0.09805417 0.0214000 0.10736667 0.08596667 0.231301188 0.009173587 FALSE TRUE
MSTRG.23583.2 ENSG00000114520 HEK293_OSMI2_6hA HEK293_TMG_6hB SNX4 protein_coding   17.58103 3.270015 28.16176 0.452941 0.7091687 3.102476 0.7880649 0.02258905 2.236032 0.02258905 0.5360436 6.106847 0.03417500 0.0070000 0.07903333 0.07203333 0.009173587 0.009173587 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000114520 E001 128.3732308 0.0070295751 0.003908541 0.02864045 3 125446650 125447150 501 - 1.917 2.073 0.524
ENSG00000114520 E002 166.9452285 0.0002861124 0.612381471 0.77845739 3 125447151 125447467 317 - 2.052 2.076 0.080
ENSG00000114520 E003 98.6628652 0.0017345536 0.808383730 0.90122729 3 125447468 125447710 243 - 1.829 1.819 -0.032
ENSG00000114520 E004 91.2250781 0.0004380480 0.895143912 0.94929325 3 125447711 125447826 116 - 1.796 1.808 0.040
ENSG00000114520 E005 0.3337900 0.0272718589 0.163616752   3 125449070 125449255 186 - 0.051 0.266 2.775
ENSG00000114520 E006 3.2718095 0.0713298586 0.697015679 0.83363140 3 125449297 125449411 115 - 0.523 0.429 -0.470
ENSG00000114520 E007 134.5798451 0.0003416175 0.263069839 0.50228581 3 125451305 125451419 115 - 1.957 2.009 0.175
ENSG00000114520 E008 150.6624688 0.0025202050 0.121455081 0.31818854 3 125453810 125453955 146 - 2.004 2.077 0.246
ENSG00000114520 E009 123.9311305 0.0020554577 0.145534468 0.35570227 3 125457266 125457365 100 - 1.920 1.994 0.248
ENSG00000114520 E010 85.0741694 0.0005200769 0.686393403 0.82674165 3 125460771 125460860 90 - 1.764 1.791 0.089
ENSG00000114520 E011 0.1308682 0.0326491905 0.049866067   3 125469444 125469453 10 - 0.000 0.265 11.924
ENSG00000114520 E012 80.2527632 0.0007025452 0.761713944 0.87365813 3 125469454 125469519 66 - 1.739 1.760 0.072
ENSG00000114520 E013 7.7595786 0.0048930647 0.299940613 0.54035420 3 125470566 125470661 96 - 0.816 0.641 -0.719
ENSG00000114520 E014 85.5448471 0.0029958436 0.830420542 0.91402235 3 125476695 125476756 62 - 1.770 1.760 -0.036
ENSG00000114520 E015 105.5232496 0.0004226541 0.227894999 0.46284622 3 125480247 125480319 73 - 1.865 1.808 -0.192
ENSG00000114520 E016 97.6921285 0.0009379928 0.124096254 0.32221443 3 125489408 125489463 56 - 1.834 1.753 -0.271
ENSG00000114520 E017 93.8684930 0.0050055082 0.077890537 0.24060252 3 125497341 125497388 48 - 1.819 1.705 -0.385
ENSG00000114520 E018 134.7781745 0.0039895788 0.061959457 0.20718241 3 125497834 125497983 150 - 1.973 1.875 -0.330
ENSG00000114520 E019 121.9298067 0.0003566327 0.009745318 0.05702695 3 125498059 125498194 136 - 1.936 1.814 -0.411
ENSG00000114520 E020 103.5690412 0.0004707206 0.113048292 0.30424558 3 125504623 125504744 122 - 1.862 1.784 -0.262
ENSG00000114520 E021 64.4036739 0.0006320463 0.218948008 0.45219318 3 125520032 125520279 248 - 1.640 1.720 0.269