ENSG00000109536

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000226798 ENSG00000109536 HEK293_OSMI2_6hA HEK293_TMG_6hB FRG1 protein_coding protein_coding 27.2407 21.05343 32.27083 7.340321 2.260084 0.6159375 22.349214 18.682608 24.744914 6.4424071 1.7221637 0.4052471 0.8319042 0.88743333 0.7668333 -0.12060000 0.01157888 0.01157888 FALSE TRUE
MSTRG.25841.1 ENSG00000109536 HEK293_OSMI2_6hA HEK293_TMG_6hB FRG1 protein_coding   27.2407 21.05343 32.27083 7.340321 2.260084 0.6159375 3.866307 1.909751 5.701448 0.8573802 0.1721758 1.5729373 0.1290500 0.08183333 0.1778000 0.09596667 0.02196986 0.01157888 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000109536 E001 15.6235538 0.0031142319 0.0009166058 0.009234191 4 189940859 189940869 11 + 1.340 1.043 -1.056
ENSG00000109536 E002 16.6765361 0.0025221569 0.0044622147 0.031727796 4 189940870 189940871 2 + 1.351 1.108 -0.857
ENSG00000109536 E003 29.7325023 0.0072250837 0.0070698542 0.044947539 4 189940872 189940898 27 + 1.576 1.381 -0.671
ENSG00000109536 E004 31.4159506 0.0087514921 0.0058715240 0.039110407 4 189940899 189940900 2 + 1.602 1.399 -0.698
ENSG00000109536 E005 112.3011608 0.0051643594 0.8755910503 0.939067959 4 189940901 189941023 123 + 2.065 2.051 -0.048
ENSG00000109536 E006 110.7352958 0.0034600686 0.8892908169 0.946342290 4 189941024 189941071 48 + 2.056 2.043 -0.042
ENSG00000109536 E007 0.0000000       4 189941833 189941908 76 +      
ENSG00000109536 E008 0.4466850 0.0236809592 0.1428836414   4 189941909 189942000 92 + 0.271 0.000 -10.728
ENSG00000109536 E009 0.2852693 0.3265408633 0.4148419226   4 189942001 189942004 4 + 0.195 0.000 -10.118
ENSG00000109536 E010 130.5515308 0.0003406673 0.9372139325 0.970803450 4 189943202 189943272 71 + 2.123 2.125 0.007
ENSG00000109536 E011 112.3184989 0.0004605519 0.5804058472 0.757450393 4 189952162 189952200 39 + 2.054 2.073 0.062
ENSG00000109536 E012 144.3699272 0.0004827771 0.4812664277 0.687909763 4 189952201 189952287 87 + 2.160 2.180 0.065
ENSG00000109536 E013 137.9984368 0.0005073021 0.2007822218 0.430697242 4 189953068 189953125 58 + 2.129 2.165 0.123
ENSG00000109536 E014 176.1222025 0.0013753022 0.1894089446 0.416215874 4 189955037 189955151 115 + 2.235 2.270 0.117
ENSG00000109536 E015 151.9207899 0.0005075182 0.2912228642 0.531798687 4 189957398 189957502 105 + 2.175 2.204 0.097
ENSG00000109536 E016 114.6982117 0.0016081966 0.7724637576 0.880139236 4 189960748 189960839 92 + 2.071 2.060 -0.036
ENSG00000109536 E017 2.5180535 0.0093481701 0.1150656452 0.307588551 4 189960862 189961081 220 + 0.651 0.387 -1.274
ENSG00000109536 E018 3.4932528 0.0092820220 0.4702698966 0.680272617 4 189961082 189961200 119 + 0.703 0.588 -0.494
ENSG00000109536 E019 1.4094976 0.3944001069 0.2134972992 0.445803488 4 189961429 189961550 122 + 0.265 0.500 1.360
ENSG00000109536 E020 92.9036389 0.0014863029 0.4767338390 0.684833053 4 189961822 189961932 111 + 1.964 1.992 0.096
ENSG00000109536 E021 53.3408786 0.0009253072 0.6850213114 0.825918891 4 189963093 189963205 113 + 1.734 1.755 0.070