ENSG00000109475

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394665 ENSG00000109475 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL34 protein_coding protein_coding 1035.293 371.2118 1198.498 70.27735 82.85987 1.690886 178.8915 44.18094 231.2297 5.531774 16.22385 2.387566 0.168025 0.1228 0.1929667 0.07016667 0.03734074 0.03734074 FALSE  
ENST00000394667 ENSG00000109475 HEK293_OSMI2_6hA HEK293_TMG_6hB RPL34 protein_coding protein_coding 1035.293 371.2118 1198.498 70.27735 82.85987 1.690886 833.7323 321.35230 941.1974 64.641074 63.07355 1.550312 0.810575 0.8610 0.7855667 -0.07543333 0.08011925 0.03734074 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000109475 E001 1.2601087 4.927284e-02 8.876226e-01   4 108620482 108620539 58 + 0.342 0.309 -0.209
ENSG00000109475 E002 26.1593055 3.270318e-03 1.658353e-02 8.391397e-02 4 108620540 108620568 29 + 1.441 1.233 -0.726
ENSG00000109475 E003 27.4266163 2.236131e-03 1.584091e-02 8.121947e-02 4 108620569 108620575 7 + 1.460 1.258 -0.701
ENSG00000109475 E004 528.9582382 9.892057e-04 2.050216e-03 1.739738e-02 4 108620576 108620589 14 + 2.660 2.723 0.213
ENSG00000109475 E005 1366.1258849 6.815758e-04 1.541286e-13 1.845880e-11 4 108620590 108620600 11 + 3.056 3.166 0.365
ENSG00000109475 E006 57.9272649 8.638551e-03 7.327337e-01 8.558199e-01 4 108620601 108620625 25 + 1.733 1.700 -0.112
ENSG00000109475 E007 68.2790214 9.412766e-03 4.653129e-01 6.767780e-01 4 108620626 108620632 7 + 1.810 1.752 -0.197
ENSG00000109475 E008 154.4894664 1.021867e-03 2.451457e-03 2.001701e-02 4 108620633 108620655 23 + 2.176 2.058 -0.396
ENSG00000109475 E009 235.6115291 7.934064e-03 1.214529e-01 3.181885e-01 4 108620656 108620712 57 + 2.352 2.264 -0.295
ENSG00000109475 E010 53.3794659 1.430048e-02 1.944687e-02 9.400336e-02 4 108620713 108621630 918 + 1.631 1.804 0.585
ENSG00000109475 E011 8.9024662 3.588573e-03 3.129973e-01 5.533103e-01 4 108621631 108621696 66 + 0.992 0.859 -0.502
ENSG00000109475 E012 30.9181592 4.104889e-03 9.430664e-01 9.738551e-01 4 108621697 108621950 254 + 1.458 1.456 -0.008
ENSG00000109475 E013 3106.6898039 6.285348e-04 2.992837e-06 7.417581e-05 4 108621951 108622024 74 + 3.431 3.484 0.177
ENSG00000109475 E014 6.3716997 1.322167e-02 7.346708e-01 8.570846e-01 4 108622043 108622104 62 + 0.821 0.859 0.147
ENSG00000109475 E015 5044.6947152 5.010017e-05 9.446822e-01 9.746835e-01 4 108622105 108622204 100 + 3.659 3.652 -0.024
ENSG00000109475 E016 17.5502402 2.199547e-03 1.218555e-01 3.188283e-01 4 108622205 108622514 310 + 1.177 1.306 0.452
ENSG00000109475 E017 4989.3788531 4.648540e-04 7.492315e-04 7.854120e-03 4 108622515 108622618 104 + 3.661 3.629 -0.107
ENSG00000109475 E018 30.2802691 5.386642e-02 6.700318e-01 8.163198e-01 4 108622619 108623332 714 + 1.435 1.490 0.191
ENSG00000109475 E019 3045.6570701 1.294688e-03 2.334864e-04 3.040515e-03 4 108625128 108625253 126 + 3.454 3.395 -0.195
ENSG00000109475 E020 4.2465249 6.260142e-03 8.994591e-05 1.367492e-03 4 108625254 108625257 4 + 0.448 0.992 2.290
ENSG00000109475 E021 0.9418907 4.699896e-02 5.601501e-01   4 108625258 108625370 113 + 0.301 0.182 -0.946
ENSG00000109475 E022 2.1274743 2.023801e-02 3.165548e-01 5.565657e-01 4 108625431 108625554 124 + 0.506 0.310 -1.086
ENSG00000109475 E023 88.3694969 5.696655e-04 1.003866e-09 5.856392e-08 4 108630021 108630481 461 + 1.976 1.678 -1.004