ENSG00000108733

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000225873 ENSG00000108733 HEK293_OSMI2_6hA HEK293_TMG_6hB PEX12 protein_coding protein_coding 3.145019 2.454851 3.944165 0.2933248 0.118528 0.6818725 2.4495186 1.5534537 3.5870357 0.2087718 0.1112059 1.202072 0.7611708 0.6303667 0.91213333 0.2817667 0.0368007 0.0368007 FALSE TRUE
ENST00000586663 ENSG00000108733 HEK293_OSMI2_6hA HEK293_TMG_6hB PEX12 protein_coding non_stop_decay 3.145019 2.454851 3.944165 0.2933248 0.118528 0.6818725 0.6351203 0.8476217 0.2573002 0.1432893 0.2573002 -1.681881 0.2207500 0.3410000 0.06193333 -0.2790667 0.1723086 0.0368007 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000108733 E001 61.7299645 0.002758076 0.004563253 0.03227783 17 35574795 35575788 994 - 1.840 1.716 -0.420
ENSG00000108733 E002 26.4154848 0.013722398 0.785294993 0.88769607 17 35575789 35575927 139 - 1.431 1.416 -0.053
ENSG00000108733 E003 32.7844087 0.007274192 0.618947253 0.78306995 17 35575928 35576181 254 - 1.529 1.500 -0.099
ENSG00000108733 E004 11.5793071 0.050772441 0.597268886 0.76851717 17 35577038 35577054 17 - 1.124 1.042 -0.297
ENSG00000108733 E005 31.3767050 0.001272422 0.925369053 0.96487053 17 35577055 35577247 193 - 1.507 1.503 -0.015
ENSG00000108733 E006 40.0128435 0.001097231 0.241506359 0.47826173 17 35577248 35577591 344 - 1.582 1.644 0.210
ENSG00000108733 E007 28.4452875 0.005257760 0.027537461 0.11990089 17 35577896 35578077 182 - 1.398 1.547 0.512
ENSG00000108733 E008 37.3688494 0.018322956 0.222418629 0.45636303 17 35578078 35578571 494 - 1.541 1.635 0.322
ENSG00000108733 E009 0.6356275 0.021337350 0.492487802   17 35578686 35578863 178 - 0.260 0.140 -1.105