ENSG00000104219

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262096 ENSG00000104219 HEK293_OSMI2_6hA HEK293_TMG_6hB ZDHHC2 protein_coding protein_coding 7.880612 3.335475 10.96438 0.6262291 1.550058 1.713857 1.607462 1.018114 0.000000 0.2059238 0.0000000 -6.683856 0.2073875 0.3035333 0.0000000 -0.3035333 7.513581e-21 7.513581e-21 FALSE TRUE
ENST00000522184 ENSG00000104219 HEK293_OSMI2_6hA HEK293_TMG_6hB ZDHHC2 protein_coding protein_coding 7.880612 3.335475 10.96438 0.6262291 1.550058 1.713857 4.766167 2.016331 6.410675 0.1856223 0.8730883 1.663855 0.6150417 0.6285333 0.5854333 -0.0431000 9.087797e-01 7.513581e-21 FALSE FALSE
MSTRG.31206.2 ENSG00000104219 HEK293_OSMI2_6hA HEK293_TMG_6hB ZDHHC2 protein_coding   7.880612 3.335475 10.96438 0.6262291 1.550058 1.713857 1.100785 0.000000 3.798655 0.0000000 0.2424476 8.573138 0.1295125 0.0000000 0.3548667 0.3548667 3.187125e-15 7.513581e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000104219 E001 0.5649788 0.0458601608 3.965554e-03   8 17156482 17156511 30 + 0.000 0.480 13.493
ENSG00000104219 E002 0.4341107 0.0237883011 1.256184e-02   8 17156512 17156513 2 + 0.000 0.402 15.349
ENSG00000104219 E003 0.8682213 0.1126570676 8.224351e-04   8 17156514 17156597 84 + 0.000 0.601 15.658
ENSG00000104219 E004 2.3329466 0.0191572457 1.724260e-05 3.368059e-04 8 17156598 17156686 89 + 0.149 0.852 3.899
ENSG00000104219 E005 2.0718305 0.0117688435 1.126236e-03 1.086436e-02 8 17156687 17156698 12 + 0.208 0.747 2.902
ENSG00000104219 E006 18.8831291 0.0154208774 3.300271e-01 5.689622e-01 8 17156699 17156853 155 + 1.219 1.322 0.361
ENSG00000104219 E007 0.1614157 0.0341026056 1.000000e+00   8 17157434 17157573 140 + 0.081 0.000 -12.649
ENSG00000104219 E008 0.0000000       8 17162799 17162889 91 +      
ENSG00000104219 E009 21.6558324 0.0017583300 4.756874e-02 1.739595e-01 8 17184789 17184815 27 + 1.260 1.414 0.537
ENSG00000104219 E010 37.8427400 0.0009613578 9.112443e-01 9.578262e-01 8 17186331 17186425 95 + 1.551 1.545 -0.021
ENSG00000104219 E011 0.4820342 0.0214325831 3.487604e-01   8 17189880 17189914 35 + 0.208 0.000 -14.307
ENSG00000104219 E012 54.6097854 0.0007226541 2.180828e-01 4.511226e-01 8 17195504 17195624 121 + 1.724 1.660 -0.215
ENSG00000104219 E013 49.8567185 0.0010036475 7.564465e-01 8.705040e-01 8 17197582 17197651 70 + 1.676 1.660 -0.054
ENSG00000104219 E014 36.5897325 0.0011366066 2.644435e-01 5.036798e-01 8 17198381 17198413 33 + 1.512 1.581 0.238
ENSG00000104219 E015 56.7926721 0.0008323245 8.303073e-02 2.508187e-01 8 17205655 17205775 121 + 1.693 1.778 0.290
ENSG00000104219 E016 35.8064405 0.0011151751 2.363178e-01 4.723191e-01 8 17207960 17208012 53 + 1.501 1.575 0.255
ENSG00000104219 E017 20.9201889 0.0030798781 8.438764e-01 9.215363e-01 8 17208013 17208017 5 + 1.302 1.286 -0.056
ENSG00000104219 E018 50.3508407 0.0007418081 8.506390e-01 9.253844e-01 8 17208018 17208092 75 + 1.669 1.679 0.037
ENSG00000104219 E019 77.9684540 0.0007640643 2.043947e-01 4.351047e-01 8 17209932 17210058 127 + 1.845 1.899 0.183
ENSG00000104219 E020 0.0000000       8 17210059 17210185 127 +      
ENSG00000104219 E021 67.0749609 0.0005652401 4.849000e-02 1.761776e-01 8 17210388 17210480 93 + 1.767 1.855 0.298
ENSG00000104219 E022 65.6928638 0.0005887497 8.477482e-04 8.674600e-03 8 17215237 17215349 113 + 1.737 1.885 0.499
ENSG00000104219 E023 39.3685021 0.0008936095 3.517905e-03 2.645212e-02 8 17217172 17217246 75 + 1.512 1.679 0.570
ENSG00000104219 E024 179.9938384 0.0006877699 5.625289e-24 2.595934e-21 8 17220256 17224799 4544 + 2.288 1.976 -1.044