ENSG00000103642

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261893 ENSG00000103642 HEK293_OSMI2_6hA HEK293_TMG_6hB LACTB protein_coding protein_coding 7.659455 2.982426 13.26462 0.3709671 0.9057137 2.149283 4.2857257 2.3661086 6.8333319 0.5034530 0.27382784 1.526098 0.5874458 0.77373333 0.51726667 -0.2564667 1.963329e-01 1.457639e-07 FALSE TRUE
ENST00000413507 ENSG00000103642 HEK293_OSMI2_6hA HEK293_TMG_6hB LACTB protein_coding protein_coding 7.659455 2.982426 13.26462 0.3709671 0.9057137 2.149283 0.7770047 0.3908906 0.9871581 0.1060495 0.02549303 1.314614 0.1105333 0.12693333 0.07513333 -0.0518000 3.830241e-01 1.457639e-07 FALSE TRUE
ENST00000557972 ENSG00000103642 HEK293_OSMI2_6hA HEK293_TMG_6hB LACTB protein_coding protein_coding 7.659455 2.982426 13.26462 0.3709671 0.9057137 2.149283 1.7560561 0.2254265 3.8480543 0.2254265 0.42791676 4.034525 0.1721333 0.09936667 0.28836667 0.1890000 3.234692e-01 1.457639e-07 FALSE FALSE
ENST00000559782 ENSG00000103642 HEK293_OSMI2_6hA HEK293_TMG_6hB LACTB protein_coding retained_intron 7.659455 2.982426 13.26462 0.3709671 0.9057137 2.149283 0.8406688 0.0000000 1.5960728 0.0000000 0.21433304 7.327394 0.1298792 0.00000000 0.11920000 0.1192000 1.457639e-07 1.457639e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000103642 E001 4.4620568 0.0939268224 0.890481283 0.94693861 15 63121833 63121860 28 + 0.687 0.722 0.144
ENSG00000103642 E002 22.4225397 0.0034251252 0.750193523 0.86687385 15 63121861 63122228 368 + 1.316 1.346 0.103
ENSG00000103642 E003 1.3166394 0.0199305014 0.362887384   15 63122583 63122635 53 + 0.369 0.189 -1.294
ENSG00000103642 E004 27.8614543 0.0014891467 0.182594085 0.40741920 15 63122636 63122702 67 + 1.434 1.336 -0.341
ENSG00000103642 E005 58.8523621 0.0006870542 0.092005966 0.26743225 15 63126859 63127049 191 + 1.747 1.664 -0.281
ENSG00000103642 E006 95.8165690 0.0004734332 0.523092224 0.71714195 15 63127353 63127689 337 + 1.940 1.917 -0.075
ENSG00000103642 E007 0.5775531 0.0202388267 1.000000000   15 63129359 63129484 126 + 0.197 0.189 -0.072
ENSG00000103642 E008 57.8274594 0.0006650301 0.590976973 0.76436191 15 63129485 63129650 166 + 1.724 1.699 -0.086
ENSG00000103642 E009 29.6857145 0.0018065109 0.005574339 0.03760132 15 63129651 63131471 1821 + 1.383 1.567 0.634
ENSG00000103642 E010 112.4901077 0.0005623733 0.218834801 0.45202436 15 63141280 63142061 782 + 1.990 2.030 0.134