ENSG00000103353

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395878 ENSG00000103353 HEK293_OSMI2_6hA HEK293_TMG_6hB UBFD1 protein_coding protein_coding 46.6317 48.60227 47.66771 4.477103 1.52493 -0.02800576 28.25307 22.40487 35.295025 1.011156 1.996650 0.6554172 0.6134292 0.4726000 0.7396000 0.2670000 0.02936145 0.02936145 FALSE TRUE
ENST00000563366 ENSG00000103353 HEK293_OSMI2_6hA HEK293_TMG_6hB UBFD1 protein_coding processed_transcript 46.6317 48.60227 47.66771 4.477103 1.52493 -0.02800576 11.27845 19.14848 5.281209 5.601775 1.231754 -1.8563142 0.2323542 0.3809667 0.1113333 -0.2696333 0.03114636 0.02936145 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000103353 E001 2.249264 0.0107124364 1.677707e-01 3.876383e-01 16 23557549 23557623 75 + 0.340 0.653 1.555
ENSG00000103353 E002 9.296365 0.1208517416 2.686643e-01 5.083274e-01 16 23557624 23557720 97 + 0.852 1.143 1.074
ENSG00000103353 E003 9.761831 0.1676169750 4.030227e-01 6.307532e-01 16 23557721 23557726 6 + 0.901 1.144 0.891
ENSG00000103353 E004 13.311461 0.0640412344 2.300054e-01 4.651576e-01 16 23557727 23557736 10 + 1.006 1.279 0.982
ENSG00000103353 E005 17.428883 0.0378754227 4.364870e-01 6.561349e-01 16 23557737 23557745 9 + 1.164 1.362 0.698
ENSG00000103353 E006 32.620490 0.0397502857 1.989459e-01 4.283960e-01 16 23557746 23557766 21 + 1.390 1.646 0.877
ENSG00000103353 E007 32.245381 0.0420467878 2.322482e-01 4.678381e-01 16 23557767 23557767 1 + 1.390 1.638 0.852
ENSG00000103353 E008 2.282778 0.3041876197 9.261564e-01 9.652145e-01 16 23557768 23557949 182 + 0.502 0.500 -0.009
ENSG00000103353 E009 237.686332 0.0058951117 1.053416e-01 2.912724e-01 16 23557950 23558279 330 + 2.352 2.405 0.179
ENSG00000103353 E010 3.597809 0.0564420674 1.249873e-01 3.237158e-01 16 23559028 23559467 440 + 0.715 0.543 -0.747
ENSG00000103353 E011 346.676485 0.0043194947 5.587531e-08 2.204161e-06 16 23559468 23559676 209 + 2.572 2.501 -0.234
ENSG00000103353 E012 6.809379 0.0043198342 8.654306e-02 2.574922e-01 16 23559779 23559868 90 + 0.942 0.841 -0.383
ENSG00000103353 E013 3.982722 0.1515625026 5.494819e-01 7.359270e-01 16 23561665 23561740 76 + 0.692 0.690 -0.009
ENSG00000103353 E014 6.809714 0.0098932813 6.060263e-01 7.742307e-01 16 23561833 23562205 373 + 0.861 0.904 0.164
ENSG00000103353 E015 216.321777 0.0005271947 4.257187e-14 5.685955e-12 16 23562206 23562271 66 + 2.367 2.293 -0.248
ENSG00000103353 E016 288.160403 0.0023149437 1.412420e-10 9.714924e-09 16 23562625 23562730 106 + 2.491 2.418 -0.244
ENSG00000103353 E017 12.425445 0.0034583262 1.641637e-01 3.829029e-01 16 23564039 23564109 71 + 1.131 1.110 -0.074
ENSG00000103353 E018 13.018111 0.1591245545 7.497017e-01 8.665885e-01 16 23564460 23564732 273 + 1.018 1.262 0.873
ENSG00000103353 E019 53.233813 0.1308474988 9.195930e-01 9.620012e-01 16 23564964 23566986 2023 + 1.602 1.847 0.827
ENSG00000103353 E020 292.537318 0.0018584841 5.303995e-12 4.808178e-10 16 23566987 23567069 83 + 2.498 2.424 -0.248
ENSG00000103353 E021 4883.225788 0.0059018171 2.374572e-06 6.060368e-05 16 23570480 23574389 3910 + 3.596 3.773 0.590