ENSG00000103319

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263026 ENSG00000103319 HEK293_OSMI2_6hA HEK293_TMG_6hB EEF2K protein_coding protein_coding 7.203219 6.038431 8.445008 0.7011839 0.4942156 0.4832452 4.4531525 4.3515300 4.7059260 0.7473307 0.0926216 0.1127068 0.63742500 0.71070000 0.56153333 -0.1491666667 0.42237696 0.01181628 FALSE TRUE
ENST00000563555 ENSG00000103319 HEK293_OSMI2_6hA HEK293_TMG_6hB EEF2K protein_coding retained_intron 7.203219 6.038431 8.445008 0.7011839 0.4942156 0.4832452 0.4235406 0.2092819 0.3797491 0.1517659 0.3797491 0.8297590 0.05735833 0.04120000 0.04053333 -0.0006666667 0.84173560 0.01181628 FALSE TRUE
ENST00000568269 ENSG00000103319 HEK293_OSMI2_6hA HEK293_TMG_6hB EEF2K protein_coding nonsense_mediated_decay 7.203219 6.038431 8.445008 0.7011839 0.4942156 0.4832452 0.4231226 0.3125894 0.5522464 0.0615265 0.0400703 0.8015034 0.05615000 0.05473333 0.06593333 0.0112000000 0.89564183 0.01181628 TRUE TRUE
MSTRG.12247.4 ENSG00000103319 HEK293_OSMI2_6hA HEK293_TMG_6hB EEF2K protein_coding   7.203219 6.038431 8.445008 0.7011839 0.4942156 0.4832452 1.7735767 1.1650298 2.8070869 0.1382953 0.1944836 1.2615068 0.22865417 0.19333333 0.33200000 0.1386666667 0.01181628 0.01181628 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000103319 E001 1.664597 0.0133593835 2.608109e-01 4.998147e-01 16 22206278 22206295 18 + 0.500 0.304 -1.094
ENSG00000103319 E002 7.651284 0.0039326488 9.968517e-01 1.000000e+00 16 22206296 22206325 30 + 0.924 0.926 0.006
ENSG00000103319 E003 13.891714 0.0025770222 8.694889e-01 9.355941e-01 16 22206326 22206394 69 + 1.154 1.172 0.063
ENSG00000103319 E004 32.692384 0.0015953315 8.924022e-01 9.478027e-01 16 22206395 22206679 285 + 1.519 1.529 0.032
ENSG00000103319 E005 63.217331 0.0140345868 3.055628e-01 5.462101e-01 16 22225654 22225975 322 + 1.843 1.766 -0.260
ENSG00000103319 E006 44.857627 0.0017661242 2.931059e-01 5.338246e-01 16 22244630 22244730 101 + 1.686 1.629 -0.193
ENSG00000103319 E007 41.192281 0.0148336690 3.194798e-01 5.592323e-01 16 22248755 22248815 61 + 1.654 1.578 -0.259
ENSG00000103319 E008 31.484720 0.0011990094 2.940898e-01 5.346781e-01 16 22250654 22250691 38 + 1.532 1.468 -0.217
ENSG00000103319 E009 55.805648 0.0084647887 8.339845e-02 2.515805e-01 16 22251151 22251322 172 + 1.792 1.697 -0.322
ENSG00000103319 E010 58.403330 0.0008050162 2.330176e-02 1.068972e-01 16 22256748 22256897 150 + 1.814 1.711 -0.350
ENSG00000103319 E011 58.032004 0.0006178015 1.221868e-01 3.193065e-01 16 22257253 22257385 133 + 1.797 1.727 -0.236
ENSG00000103319 E012 25.079052 0.0017377141 6.224007e-01 7.854788e-01 16 22257643 22257643 1 + 1.425 1.392 -0.112
ENSG00000103319 E013 48.635538 0.0007344438 3.277556e-01 5.668246e-01 16 22257644 22257770 127 + 1.710 1.662 -0.162
ENSG00000103319 E014 84.544987 0.0005146084 3.270801e-02 1.352497e-01 16 22258494 22258695 202 + 1.964 1.883 -0.272
ENSG00000103319 E015 3.941205 0.0613190946 4.952942e-01 6.976630e-01 16 22258696 22258827 132 + 0.643 0.753 0.458
ENSG00000103319 E016 51.787993 0.0027793964 2.653739e-01 5.047554e-01 16 22260462 22260529 68 + 1.747 1.688 -0.197
ENSG00000103319 E017 52.844310 0.0007416944 2.574736e-01 4.962145e-01 16 22263110 22263187 78 + 1.752 1.699 -0.181
ENSG00000103319 E018 1.030445 0.1504472947 6.713511e-01   16 22263188 22263322 135 + 0.284 0.365 0.516
ENSG00000103319 E019 49.231083 0.0008703254 2.406701e-02 1.092904e-01 16 22264818 22264880 63 + 1.745 1.632 -0.382
ENSG00000103319 E020 6.641490 0.0630018647 8.553253e-01 9.277962e-01 16 22264881 22265245 365 + 0.873 0.904 0.119
ENSG00000103319 E021 54.903598 0.0009223488 1.419707e-01 3.504038e-01 16 22266390 22266524 135 + 1.771 1.702 -0.233
ENSG00000103319 E022 64.525332 0.0006195084 8.003213e-01 8.965088e-01 16 22266688 22266876 189 + 1.801 1.814 0.044
ENSG00000103319 E023 58.474134 0.0006425014 6.558933e-01 8.072719e-01 16 22273626 22273750 125 + 1.775 1.757 -0.063
ENSG00000103319 E024 70.891462 0.0005877742 4.072879e-01 6.339316e-01 16 22280198 22280376 179 + 1.864 1.832 -0.110
ENSG00000103319 E025 547.712913 0.0003746932 1.523949e-01 3.658126e-01 16 22283887 22286557 2671 + 2.719 2.745 0.085
ENSG00000103319 E026 67.805725 0.0167437595 2.683697e-03 2.147907e-02 16 22286558 22287037 480 + 1.706 1.939 0.788
ENSG00000103319 E027 13.889339 0.0307412898 8.182857e-04 8.432777e-03 16 22287038 22287201 164 + 0.923 1.338 1.494
ENSG00000103319 E028 130.975454 0.0003703035 8.968646e-11 6.423797e-09 16 22287202 22288738 1537 + 2.000 2.204 0.684