ENSG00000101654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262173 ENSG00000101654 HEK293_OSMI2_6hA HEK293_TMG_6hB RNMT protein_coding protein_coding 7.750709 2.683757 14.88191 0.2371258 0.5180119 2.466837 0.4837055 1.12150473 0.000000 0.10144988 0.0000000 -6.822099 0.18097917 0.42640000 0.00000000 -0.42640000 5.967554e-20 5.967554e-20 FALSE TRUE
ENST00000383314 ENSG00000101654 HEK293_OSMI2_6hA HEK293_TMG_6hB RNMT protein_coding protein_coding 7.750709 2.683757 14.88191 0.2371258 0.5180119 2.466837 4.0833933 0.14108787 9.516661 0.09713905 0.4839600 5.978511 0.34183750 0.05153333 0.63903333 0.58750000 1.622834e-04 5.967554e-20 FALSE TRUE
ENST00000592764 ENSG00000101654 HEK293_OSMI2_6hA HEK293_TMG_6hB RNMT protein_coding protein_coding 7.750709 2.683757 14.88191 0.2371258 0.5180119 2.466837 1.8470983 0.85842618 3.340063 0.48540606 0.4404668 1.947713 0.24568333 0.29326667 0.22460000 -0.06866667 1.000000e+00 5.967554e-20 FALSE TRUE
ENST00000593007 ENSG00000101654 HEK293_OSMI2_6hA HEK293_TMG_6hB RNMT protein_coding protein_coding 7.750709 2.683757 14.88191 0.2371258 0.5180119 2.466837 0.3922022 0.46947908 0.000000 0.24056931 0.0000000 -5.583396 0.12475833 0.19046667 0.00000000 -0.19046667 9.161772e-02 5.967554e-20 FALSE FALSE
MSTRG.15535.2 ENSG00000101654 HEK293_OSMI2_6hA HEK293_TMG_6hB RNMT protein_coding   7.750709 2.683757 14.88191 0.2371258 0.5180119 2.466837 0.6034907 0.07055715 1.344527 0.03528840 0.6723276 4.071633 0.05011667 0.02806667 0.09076667 0.06270000 9.146847e-01 5.967554e-20 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000101654 E001 0.0000000       18 13726660 13726666 7 +      
ENSG00000101654 E002 0.1614157 0.0322960998 1.000000e+00   18 13726667 13726672 6 + 0.056 0.001 -6.521
ENSG00000101654 E003 6.5764639 0.0042114827 5.372620e-03 3.655887e-02 18 13726673 13726703 31 + 0.814 0.244 -2.870
ENSG00000101654 E004 16.7288059 0.0037464669 2.337532e-01 4.695131e-01 18 13726704 13726729 26 + 1.140 1.003 -0.501
ENSG00000101654 E005 0.0000000       18 13726769 13726865 97 +      
ENSG00000101654 E006 0.0000000       18 13730438 13730626 189 +      
ENSG00000101654 E007 27.7638376 0.0617186923 2.388373e-01 4.752873e-01 18 13730627 13730755 129 + 1.353 1.166 -0.659
ENSG00000101654 E008 0.1426347 0.0317723488 1.000000e+00   18 13731474 13731475 2 + 0.056 0.000 -8.393
ENSG00000101654 E009 85.1753897 1.4358288081 7.195833e-01 8.479691e-01 18 13731476 13731934 459 + 1.801 1.794 -0.025
ENSG00000101654 E010 53.4175721 0.0327698879 7.703592e-01 8.788707e-01 18 13734464 13734599 136 + 1.609 1.566 -0.146
ENSG00000101654 E011 55.4772600 0.0007105105 4.234713e-01 6.462309e-01 18 13737010 13737135 126 + 1.626 1.579 -0.158
ENSG00000101654 E012 50.3506324 0.0065483469 3.604765e-01 5.960648e-01 18 13740167 13740279 113 + 1.587 1.523 -0.217
ENSG00000101654 E013 46.0674187 0.0093486824 3.952516e-02 1.535323e-01 18 13741510 13741619 110 + 1.562 1.386 -0.603
ENSG00000101654 E014 35.1498914 0.0091323034 2.235575e-02 1.037598e-01 18 13741620 13741691 72 + 1.449 1.228 -0.773
ENSG00000101654 E015 51.8868414 0.0080800280 9.815199e-03 5.732920e-02 18 13742488 13742652 165 + 1.613 1.401 -0.726
ENSG00000101654 E016 0.9750270 0.0153787590 9.706423e-01   18 13742653 13743074 422 + 0.228 0.244 0.130
ENSG00000101654 E017 36.6601784 0.0018653135 3.333675e-01 5.719488e-01 18 13746220 13746272 53 + 1.448 1.374 -0.255
ENSG00000101654 E018 44.9842197 0.0041828083 6.036353e-02 2.036457e-01 18 13746273 13746337 65 + 1.546 1.401 -0.499
ENSG00000101654 E019 0.0000000       18 13752305 13752325 21 +      
ENSG00000101654 E020 57.8489048 0.0032798263 2.918803e-02 1.249351e-01 18 13752326 13752427 102 + 1.654 1.504 -0.511
ENSG00000101654 E021 38.6219168 0.0047709811 2.822347e-04 3.550422e-03 18 13754114 13754147 34 + 1.504 1.163 -1.191
ENSG00000101654 E022 66.4371324 0.0023368709 5.616099e-03 3.780943e-02 18 13759942 13760208 267 + 1.721 1.543 -0.607
ENSG00000101654 E023 252.8003639 0.0006357446 3.379329e-02 1.383229e-01 18 13760209 13762038 1830 + 2.277 2.219 -0.196
ENSG00000101654 E024 94.0079332 0.0030117197 2.894020e-01 5.299418e-01 18 13762039 13762300 262 + 1.832 1.895 0.211
ENSG00000101654 E025 57.1074280 0.0027784065 1.301502e-01 3.320687e-01 18 13762301 13762437 137 + 1.610 1.712 0.347
ENSG00000101654 E026 374.7884714 0.0012312416 1.849435e-11 1.520952e-09 18 13762438 13764111 1674 + 2.407 2.571 0.544
ENSG00000101654 E027 87.2655573 0.0052923905 6.498059e-05 1.042236e-03 18 13764112 13764556 445 + 1.764 1.991 0.767