ENSG00000101473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000217455 ENSG00000101473 HEK293_OSMI2_6hA HEK293_TMG_6hB ACOT8 protein_coding protein_coding 38.82048 60.72076 16.97462 12.79674 0.563394 -1.838198 28.946583 45.889818 11.66117 10.2887253 1.0339632 -1.975539 0.73055833 0.74966667 0.68480000 -0.06486667 7.286582e-01 1.144327e-09 FALSE  
ENST00000457981 ENSG00000101473 HEK293_OSMI2_6hA HEK293_TMG_6hB ACOT8 protein_coding protein_coding 38.82048 60.72076 16.97462 12.79674 0.563394 -1.838198 2.240708 2.582867 1.07597 0.4246025 0.1928702 -1.255564 0.06292083 0.04443333 0.06323333 0.01880000 7.031595e-01 1.144327e-09 FALSE  
ENST00000488679 ENSG00000101473 HEK293_OSMI2_6hA HEK293_TMG_6hB ACOT8 protein_coding nonsense_mediated_decay 38.82048 60.72076 16.97462 12.79674 0.563394 -1.838198 2.482571 5.796512 0.00000 1.9881333 0.0000000 -9.181528 0.04735000 0.08820000 0.00000000 -0.08820000 1.144327e-09 1.144327e-09 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000101473 E001 2.7702208 0.0131569970 4.932012e-02 1.779558e-01 20 45841721 45841725 5 - 0.757 0.430 -1.477
ENSG00000101473 E002 7.1207999 0.0042409825 8.063074e-01 8.999271e-01 20 45841726 45841729 4 - 0.849 0.816 -0.127
ENSG00000101473 E003 11.0297105 0.0296579935 6.140067e-01 7.795489e-01 20 45841730 45841737 8 - 1.044 0.973 -0.263
ENSG00000101473 E004 41.2423366 0.2275626582 3.370343e-01 5.754114e-01 20 45841738 45841764 27 - 1.399 1.541 0.486
ENSG00000101473 E005 195.5687197 1.0014312727 3.996283e-01 6.279715e-01 20 45841765 45841956 192 - 2.012 2.213 0.670
ENSG00000101473 E006 29.6528534 0.4880353429 9.438181e-01 9.742699e-01 20 45842023 45842746 724 - 1.534 1.336 -0.684
ENSG00000101473 E007 31.2126010 0.2151065878 5.156987e-01 7.120548e-01 20 45842747 45843398 652 - 1.543 1.362 -0.626
ENSG00000101473 E008 6.6687950 0.1442457594 7.954845e-02 2.439621e-01 20 45843399 45843526 128 - 1.068 0.685 -1.475
ENSG00000101473 E009 319.9786266 0.0020726441 1.630153e-03 1.453291e-02 20 45843527 45843721 195 - 2.314 2.416 0.339
ENSG00000101473 E010 15.0619372 0.0613194384 1.205173e-01 3.166570e-01 20 45843722 45843722 1 - 1.287 1.047 -0.858
ENSG00000101473 E011 20.7963665 0.1061892506 8.530153e-02 2.551740e-01 20 45843723 45843772 50 - 1.464 1.157 -1.072
ENSG00000101473 E012 36.0818140 0.1757462966 3.677613e-02 1.463246e-01 20 45843773 45844262 490 - 1.762 1.351 -1.404
ENSG00000101473 E013 290.7208339 0.0051976255 6.974547e-01 8.339161e-01 20 45844263 45844420 158 - 2.363 2.353 -0.032
ENSG00000101473 E014 234.2073273 0.0002673492 4.701380e-01 6.801731e-01 20 45848450 45848532 83 - 2.280 2.262 -0.058
ENSG00000101473 E015 308.8381825 0.0003039488 3.279982e-01 5.670119e-01 20 45848533 45848675 143 - 2.362 2.390 0.092
ENSG00000101473 E016 3.7124620 0.2360079794 2.608307e-01 4.998257e-01 20 45848676 45849110 435 - 0.817 0.504 -1.342
ENSG00000101473 E017 0.7805771 0.3397680119 5.380651e-01   20 45853420 45853421 2 - 0.000 0.219 9.374
ENSG00000101473 E018 27.7087992 0.0057151522 1.168407e-01 3.105640e-01 20 45853422 45853922 501 - 1.458 1.325 -0.457
ENSG00000101473 E019 24.0185993 0.0038977779 3.195464e-02 1.330873e-01 20 45853923 45853979 57 - 1.436 1.261 -0.610
ENSG00000101473 E020 14.2766823 0.0059753318 2.164093e-03 1.816941e-02 20 45853980 45853984 5 - 1.313 1.004 -1.103
ENSG00000101473 E021 309.6307839 0.0002615283 5.344278e-01 7.252180e-01 20 45855159 45855292 134 - 2.396 2.384 -0.040
ENSG00000101473 E022 10.1000968 0.0039399931 2.089669e-06 5.426205e-05 20 45856783 45857187 405 - 1.313 0.806 -1.857
ENSG00000101473 E023 283.5362051 0.0002229311 4.233403e-02 1.608019e-01 20 45857188 45857405 218 - 2.302 2.359 0.188