ENSG00000101413

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373433 ENSG00000101413 HEK293_OSMI2_6hA HEK293_TMG_6hB RPRD1B protein_coding protein_coding 22.10335 14.97205 24.81697 1.546505 0.8744243 0.7286734 13.756391 7.362443 18.3182106 0.6804693 0.8988992 1.3138512 0.5981375 0.4938667 0.73776667 0.2439 7.833585e-05 7.833585e-05 FALSE TRUE
ENST00000462548 ENSG00000101413 HEK293_OSMI2_6hA HEK293_TMG_6hB RPRD1B protein_coding nonsense_mediated_decay 22.10335 14.97205 24.81697 1.546505 0.8744243 0.7286734 2.973911 2.245053 3.1280906 0.2025405 0.1243953 0.4767255 0.1340458 0.1517333 0.12663333 -0.0251 7.835464e-01 7.833585e-05 FALSE TRUE
ENST00000471511 ENSG00000101413 HEK293_OSMI2_6hA HEK293_TMG_6hB RPRD1B protein_coding processed_transcript 22.10335 14.97205 24.81697 1.546505 0.8744243 0.7286734 3.430903 3.249608 0.9537979 0.4438839 0.4779280 -1.7578958 0.1778250 0.2157667 0.03946667 -0.1763 3.590993e-01 7.833585e-05   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000101413 E001 29.8355787 0.0012702239 5.800240e-01 7.571757e-01 20 38033725 38033745 21 + 1.459 1.497 0.129
ENSG00000101413 E002 51.9632217 0.0041588144 2.702689e-01 5.102314e-01 20 38033746 38033831 86 + 1.688 1.752 0.218
ENSG00000101413 E003 103.6010549 0.0078781163 8.749797e-01 9.387188e-01 20 38033832 38034073 242 + 2.011 2.008 -0.009
ENSG00000101413 E004 53.1341275 0.0008002158 4.429647e-02 1.658348e-01 20 38034074 38034098 25 + 1.758 1.658 -0.339
ENSG00000101413 E005 61.6076310 0.0005983903 4.378430e-03 3.125507e-02 20 38040435 38040470 36 + 1.832 1.699 -0.448
ENSG00000101413 E006 81.4126964 0.0005164512 7.660719e-02 2.379628e-01 20 38040471 38040564 94 + 1.927 1.857 -0.237
ENSG00000101413 E007 114.3359697 0.0003766881 1.621926e-02 8.259575e-02 20 38048348 38048481 134 + 2.077 1.996 -0.271
ENSG00000101413 E008 0.0000000       20 38049753 38049813 61 +      
ENSG00000101413 E009 62.6473562 0.0005883304 2.559091e-02 1.140304e-01 20 38057532 38057537 6 + 1.828 1.726 -0.345
ENSG00000101413 E010 121.3969146 0.0005065916 2.111989e-02 9.972703e-02 20 38057538 38057644 107 + 2.100 2.024 -0.255
ENSG00000101413 E011 120.0437743 0.0015938813 2.662158e-02 1.171323e-01 20 38059394 38059520 127 + 2.098 2.018 -0.269
ENSG00000101413 E012 0.5283420 0.3246042022 9.048923e-01   20 38065878 38065956 79 + 0.183 0.149 -0.364
ENSG00000101413 E013 51.2267088 0.0094488069 2.589867e-01 4.978525e-01 20 38066081 38066081 1 + 1.734 1.659 -0.254
ENSG00000101413 E014 104.2773728 0.0052917601 1.989943e-01 4.284639e-01 20 38066082 38066180 99 + 2.031 1.973 -0.197
ENSG00000101413 E015 87.0121541 0.0020177133 5.415470e-02 1.894524e-01 20 38066181 38066256 76 + 1.963 1.881 -0.276
ENSG00000101413 E016 2.2094912 0.0140290758 9.730560e-01 9.884860e-01 20 38070225 38070617 393 + 0.485 0.481 -0.020
ENSG00000101413 E017 0.8681014 0.0183993724 3.490038e-01   20 38083896 38083932 37 + 0.180 0.346 1.243
ENSG00000101413 E018 5.1533916 0.1585052705 7.068530e-04 7.501556e-03 20 38085376 38085646 271 + 0.307 1.084 3.440
ENSG00000101413 E019 1245.1274576 0.0001584349 1.716112e-11 1.420942e-09 20 38089726 38092364 2639 + 3.060 3.113 0.175
ENSG00000101413 E020 0.3337900 0.0279333262 7.431840e-01   20 38124770 38125081 312 + 0.099 0.148 0.654
ENSG00000101413 E021 3.5951525 0.1562607793 5.458597e-01 7.333369e-01 20 38127297 38127780 484 + 0.612 0.706 0.401