ENSG00000101146

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371242 ENSG00000101146 HEK293_OSMI2_6hA HEK293_TMG_6hB RAE1 protein_coding protein_coding 65.97402 50.47862 72.29986 5.355045 1.693711 0.518234 4.667542 4.001000 4.695079 0.2777741 0.50122551 0.230257279 0.07146667 0.08063333 0.06470000 -0.01593333 7.431021e-01 1.176003e-47 FALSE TRUE
ENST00000395841 ENSG00000101146 HEK293_OSMI2_6hA HEK293_TMG_6hB RAE1 protein_coding protein_coding 65.97402 50.47862 72.29986 5.355045 1.693711 0.518234 26.210605 19.135750 19.222647 3.8738224 0.30851285 0.006533152 0.38629167 0.37260000 0.26600000 -0.10660000 5.025388e-01 1.176003e-47 FALSE TRUE
ENST00000429339 ENSG00000101146 HEK293_OSMI2_6hA HEK293_TMG_6hB RAE1 protein_coding protein_coding 65.97402 50.47862 72.29986 5.355045 1.693711 0.518234 5.631994 0.000000 15.475868 0.0000000 0.47809356 10.596736502 0.08357083 0.00000000 0.21436667 0.21436667 1.176003e-47 1.176003e-47 FALSE FALSE
ENST00000527947 ENSG00000101146 HEK293_OSMI2_6hA HEK293_TMG_6hB RAE1 protein_coding protein_coding 65.97402 50.47862 72.29986 5.355045 1.693711 0.518234 23.864893 21.648567 27.115350 1.9940310 1.20611005 0.324703933 0.37068333 0.43230000 0.37473333 -0.05756667 6.888680e-01 1.176003e-47 FALSE TRUE
MSTRG.20894.1 ENSG00000101146 HEK293_OSMI2_6hA HEK293_TMG_6hB RAE1 protein_coding   65.97402 50.47862 72.29986 5.355045 1.693711 0.518234 3.807671 3.439176 3.751896 0.5977044 0.06921079 0.125208275 0.05865000 0.06740000 0.05196667 -0.01543333 5.667176e-01 1.176003e-47 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000101146 E001 0.9514940 0.0182550404 9.702154e-01   20 57351155 57351222 68 + 0.296 0.292 -0.025
ENSG00000101146 E002 37.4741171 0.0012904182 2.057652e-01 4.367643e-01 20 57351223 57351251 29 + 1.609 1.540 -0.235
ENSG00000101146 E003 38.2235494 0.0028204689 1.915151e-01 4.187956e-01 20 57351252 57351254 3 + 1.619 1.545 -0.253
ENSG00000101146 E004 98.1743402 0.0004533769 1.581297e-01 3.739725e-01 20 57351255 57351273 19 + 2.014 1.969 -0.153
ENSG00000101146 E005 243.1117423 0.0009598783 9.582721e-01 9.810252e-01 20 57351274 57351358 85 + 2.384 2.388 0.015
ENSG00000101146 E006 258.8688942 0.0002604507 5.182804e-01 7.138646e-01 20 57351359 57351422 64 + 2.402 2.422 0.067
ENSG00000101146 E007 0.3393995 0.0274424043 1.930824e-01   20 57351510 57351543 34 + 0.218 0.000 -11.684
ENSG00000101146 E008 0.3393995 0.0274424043 1.930824e-01   20 57351544 57351570 27 + 0.218 0.000 -11.684
ENSG00000101146 E009 0.1614157 0.0319605240 4.760476e-01   20 57351571 57351576 6 + 0.122 0.000 -10.682
ENSG00000101146 E010 0.3393995 0.0274424043 1.930824e-01   20 57351577 57351890 314 + 0.218 0.000 -11.684
ENSG00000101146 E011 0.0000000       20 57351891 57351919 29 +      
ENSG00000101146 E012 0.3393995 0.0274424043 1.930824e-01   20 57351920 57351998 79 + 0.218 0.000 -11.684
ENSG00000101146 E013 369.8467509 0.0016368747 2.028654e-01 4.331834e-01 20 57354032 57354128 97 + 2.582 2.555 -0.089
ENSG00000101146 E014 436.7936229 0.0001329202 1.917232e-06 5.028417e-05 20 57354712 57354816 105 + 2.675 2.600 -0.251
ENSG00000101146 E015 478.8776488 0.0001986774 1.272993e-06 3.500948e-05 20 57356446 57356538 93 + 2.714 2.639 -0.251
ENSG00000101146 E016 527.1229074 0.0001441206 8.555396e-05 1.312469e-03 20 57365356 57365442 87 + 2.747 2.691 -0.185
ENSG00000101146 E017 448.8708586 0.0001931967 3.254256e-01 5.646520e-01 20 57366807 57366893 87 + 2.657 2.646 -0.037
ENSG00000101146 E018 375.1115880 0.0001714864 4.718512e-01 6.814226e-01 20 57367008 57367079 72 + 2.563 2.582 0.064
ENSG00000101146 E019 416.1344589 0.0006097207 1.321290e-01 3.351882e-01 20 57368705 57368812 108 + 2.597 2.634 0.125
ENSG00000101146 E020 147.7526178 0.0536809193 3.270543e-01 5.661592e-01 20 57371134 57373474 2341 + 2.091 2.238 0.492
ENSG00000101146 E021 540.6715910 0.0007734798 6.893644e-02 2.226468e-01 20 57373475 57373581 107 + 2.708 2.751 0.143
ENSG00000101146 E022 12.1196336 0.0294719942 1.100351e-01 2.990604e-01 20 57373582 57373662 81 + 1.205 1.011 -0.699
ENSG00000101146 E023 503.6212315 0.0003484640 1.545719e-01 3.688447e-01 20 57373663 57373738 76 + 2.683 2.715 0.104
ENSG00000101146 E024 625.8328880 0.0001518919 1.067981e-01 2.936700e-01 20 57374607 57374801 195 + 2.779 2.808 0.097
ENSG00000101146 E025 21.6011154 0.1991902248 8.198545e-01 9.080031e-01 20 57374802 57375106 305 + 1.263 1.426 0.566
ENSG00000101146 E026 26.0717693 0.0031570801 7.686267e-03 4.788543e-02 20 57376179 57376266 88 + 1.318 1.515 0.682
ENSG00000101146 E027 652.7022791 0.0006798222 1.101140e-04 1.620441e-03 20 57378013 57379211 1199 + 2.774 2.849 0.249